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Proc Natl Acad Sci U S A 1975 Dec 01;7212:4777-9.
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Translation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.

Yip CC , Hew CL , Hsu H .

RNA preparations from isolated rat pancreatic islets and from human insulinomas were injected into oocytes of Xenopus laevis which were then incubated with [3H]leucine. Acid-ethanol extracts of the oocytes were immunoprecipitated with anti-insulin serum using the double antibody technique. Sodium dodecyl sulfate disc gel electrophoresis of the immunoprecipitates showed the presence of an insulin-displaceable immunoreactive material with a molecular weight of about 18,000 in extracts from oocytes injected with RNA of 9-11 S. This immunoreactive product was not detected in extracts from oocytes injected with buffer or 4-8S RNA or RNA heavier than 11 S. These observations suggest the involvement of a precursor larger than proinsulin in the biosynthesis of insulin.

PubMed ID: 174093
PMC ID: PMC388814
Article link:

Species referenced: Xenopus laevis
Genes referenced: ins

References [+] :
Clark, Insulin biosynthesis in the rat: demonstration of two proinsulins. 1969, Pubmed