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J Physiol
2006 Dec 01;577Pt 2:513-23. doi: 10.1113/jphysiol.2006.117440.
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Activation of protein kinase C augments T-type Ca2+ channel activity without changing channel surface density.
Park JY
,
Kang HW
,
Moon HJ
,
Huh SU
,
Jeong SW
,
Soldatov NM
,
Lee JH
.
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T-type Ca2+ channels play essential roles in numerous cellular processes. Recently, we reported that phorbol-12-myristate-13-acetate (PMA) potently enhanced the current amplitude of Cav3.2 T-type channels reconstituted in Xenopus oocytes. Here, we have compared PMA modulation of the activities of Cav3.1, Cav3.2 and Cav3.3 channels, and have investigated the underlying mechanism. PMA augmented the current amplitudes of the three T-type channel isoforms, but the fold stimulations and time courses differed. The augmentation effects were not mimicked by 4alpha-PMA, an inactive stereoisomer of PMA, but were abolished by preincubation with protein kinase C (PKC) inhibitors, indicating that PMA augmented T-type channel currents via activation of oocyte PKC. The stimulation effect on Cav3.1 channel activity by PKC was mimicked by endothelin when endothelin receptor type A was coexpressed with Cav3.1 in the Xenopus oocyte system. Pharmacological studies combined with fluorescence imaging revealed that the surface density of Cav3.1 T-type channels was not significantly changed by activation of PKC. The PKC effect on Cav3.1 was localized to the cytoplasmic II-III loop using chimeric channels with individual cytoplasmic loops of Cav3.1 replaced by those of Cav2.1.
Altier,
Targeting Ca2+ channels to treat pain: T-type versus N-type.
2004, Pubmed
Altier,
Targeting Ca2+ channels to treat pain: T-type versus N-type.
2004,
Pubmed
Arnoult,
Voltage-dependent modulation of T-type calcium channels by protein tyrosine phosphorylation.
1997,
Pubmed
Bijlenga,
T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts.
2000,
Pubmed
Blumenthal,
The minK potassium channel exists in functional and nonfunctional forms when expressed in the plasma membrane of Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Bourinet,
Silencing of the Cav3.2 T-type calcium channel gene in sensory neurons demonstrates its major role in nociception.
2005,
Pubmed
Catterall,
Excitation-contraction coupling in vertebrate skeletal muscle: a tale of two calcium channels.
1991,
Pubmed
Chemin,
Neuronal T-type alpha 1H calcium channels induce neuritogenesis and expression of high-voltage-activated calcium channels in the NG108-15 cell line.
2002,
Pubmed
Chen,
Abnormal coronary function in mice deficient in alpha1H T-type Ca2+ channels.
2003,
Pubmed
Chen,
A role for T-type Ca2+ channels in the synergistic control of aldosterone production by ANG II and K+.
1999,
Pubmed
Chen,
Specific modulation of Na+ channels in hippocampal neurons by protein kinase C epsilon.
2005,
Pubmed
Chuang,
Inhibition of T-type voltage-gated calcium channels by a new scorpion toxin.
1998,
Pubmed
,
Xenbase
Cribbs,
Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family.
1998,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Dempsey,
Protein kinase C isozymes and the regulation of diverse cell responses.
2000,
Pubmed
Enyeart,
T-type Ca2+ channels are required for adrenocorticotropin-stimulated cortisol production by bovine adrenal zona fasciculata cells.
1993,
Pubmed
Furukawa,
Endothelin-1 enhances calcium entry through T-type calcium channels in cultured neonatal rat ventricular myocytes.
1992,
Pubmed
Hagiwara,
Contribution of two types of calcium currents to the pacemaker potentials of rabbit sino-atrial node cells.
1988,
Pubmed
Herbert,
Chelerythrine is a potent and specific inhibitor of protein kinase C.
1990,
Pubmed
Ho,
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
1989,
Pubmed
Huguenard,
Low-threshold calcium currents in central nervous system neurons.
1996,
Pubmed
Janssen,
T-type and L-type Ca2+ currents in canine bronchial smooth muscle: characterization and physiological roles.
1997,
Pubmed
Kennelly,
Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases.
1991,
Pubmed
Kim,
Thalamic control of visceral nociception mediated by T-type Ca2+ channels.
2003,
Pubmed
Lan,
Protein kinase C modulates NMDA receptor trafficking and gating.
2001,
Pubmed
,
Xenbase
Lee,
Cloning and expression of a novel member of the low voltage-activated T-type calcium channel family.
1999,
Pubmed
,
Xenbase
Maeno-Hikichi,
A PKC epsilon-ENH-channel complex specifically modulates N-type Ca2+ channels.
2003,
Pubmed
,
Xenbase
Mao,
Molecular basis for the inhibition of G protein-coupled inward rectifier K(+) channels by protein kinase C.
2004,
Pubmed
,
Xenbase
Marchetti,
Protein kinase activator 1-oleoyl-2-acetyl-sn-glycerol inhibits two types of calcium currents in GH3 cells.
1988,
Pubmed
Martínez,
Expression of T-type Ca(2+) channels in ventricular cells from hypertrophied rat hearts.
1999,
Pubmed
Matteson,
Properties of two types of calcium channels in clonal pituitary cells.
1986,
Pubmed
McHugh,
Inhibition of cardiac L-type calcium channels by protein kinase C phosphorylation of two sites in the N-terminal domain.
2000,
Pubmed
Murray,
Modulation of an inactivating human cardiac K+ channel by protein kinase C.
1994,
Pubmed
,
Xenbase
Murray,
Functional effects of protein kinase C activation on the human cardiac Na+ channel.
1997,
Pubmed
,
Xenbase
Newton,
Protein kinase C: structure, function, and regulation.
1995,
Pubmed
O'Dell,
Single calcium channels in rat and guinea-pig hippocampal neurons.
1991,
Pubmed
Park,
Modulation of Ca(v)3.2 T-type Ca2+ channels by protein kinase C.
2003,
Pubmed
,
Xenbase
Pemberton,
Modulation of low-threshold T-type calcium channels by the five muscarinic receptor subtypes in NIH 3T3 cells.
2000,
Pubmed
Peretz,
Modulation by protein kinase C activation of rat brain delayed-rectifier K+ channel expressed in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Perez-Reyes,
Molecular characterization of a neuronal low-voltage-activated T-type calcium channel.
1998,
Pubmed
,
Xenbase
Perez-Reyes,
Molecular physiology of low-voltage-activated t-type calcium channels.
2003,
Pubmed
Poole,
PKC-interacting proteins: from function to pharmacology.
2004,
Pubmed
Schroeder,
T-type calcium channels: heterogeneous expression in rat sensory neurons and selective modulation by phorbol esters.
1990,
Pubmed
Sheng,
Identification of a syntaxin-binding site on N-type calcium channels.
1994,
Pubmed
Singer-Lahat,
Modulation of cardiac Ca2+ channels in Xenopus oocytes by protein kinase C.
1992,
Pubmed
,
Xenbase
Son,
Acrosome reaction of human spermatozoa is mainly mediated by alpha1H T-type calcium channels.
2000,
Pubmed
Stea,
Determinants of PKC-dependent modulation of a family of neuronal calcium channels.
1995,
Pubmed
,
Xenbase
Sun,
Modulation of 5-HT3 receptor-mediated response and trafficking by activation of protein kinase C.
2003,
Pubmed
,
Xenbase
Talavera,
Mechanism of arachidonic acid modulation of the T-type Ca2+ channel alpha1G.
2004,
Pubmed
Tanabe,
Regions of the skeletal muscle dihydropyridine receptor critical for excitation-contraction coupling.
1990,
Pubmed
Toullec,
The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C.
1991,
Pubmed
Tsakiridou,
Selective increase in T-type calcium conductance of reticular thalamic neurons in a rat model of absence epilepsy.
1995,
Pubmed
Tseng,
Different effects of intracellular Ca and protein kinase C on cardiac T and L Ca currents.
1991,
Pubmed
Vasilets,
Activation of protein kinase C by phorbol ester induces downregulation of the Na+/K(+)-ATPase in oocytes of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Welsby,
A mechanism for the direct regulation of T-type calcium channels by Ca2+/calmodulin-dependent kinase II.
2003,
Pubmed
Wolfe,
T-type calcium channel regulation by specific G-protein betagamma subunits.
2003,
Pubmed
Wolfe,
Stimulation of recombinant Ca(v)3.2, T-type, Ca(2+) channel currents by CaMKIIgamma(C).
2002,
Pubmed
Xiao,
PKC isozyme selective regulation of cloned human cardiac delayed slow rectifier K current.
2003,
Pubmed
,
Xenbase
Xiao,
Evidence for functional role of epsilonPKC isozyme in the regulation of cardiac Na(+) channels.
2001,
Pubmed
,
Xenbase
Xiong,
Ca2+ currents in human colonic smooth muscle cells.
1995,
Pubmed
Yamazaki,
Regulation of recombinant cardiac cystic fibrosis transmembrane conductance regulator chloride channels by protein kinase C.
1999,
Pubmed
,
Xenbase
Yunker,
Low-voltage-activated ("T-Type") calcium channels in review.
2003,
Pubmed
Zhang,
Arachidonic acid modulation of alpha1H, a cloned human T-type calcium channel.
2000,
Pubmed
Zheng,
Protein kinase C potentiation of N-methyl-D-aspartate receptor activity is not mediated by phosphorylation of N-methyl-D-aspartate receptor subunits.
1999,
Pubmed
,
Xenbase
Zhou,
T-type calcium current in latent pacemaker cells isolated from cat right atrium.
1994,
Pubmed
Zhuang,
Cloning of a T-type Ca2+ channel isoform in insulin-secreting cells.
2000,
Pubmed
,
Xenbase
