Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nat Struct Mol Biol
2006 Nov 01;1311:987-95. doi: 10.1038/nsmb1164.
Show Gene links
Show Anatomy links
Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer.
Pioletti M
,
Findeisen F
,
Hura GL
,
Minor DL
.
???displayArticle.abstract??? Brain I(A) and cardiac I(to) currents arise from complexes containing Kv4 voltage-gated potassium channels and cytoplasmic calcium-sensor proteins (KChIPs). Here, we present X-ray crystallographic and small-angle X-ray scattering data that show that the KChIP1-Kv4.3 N-terminal cytoplasmic domain complex is a cross-shaped octamer bearing two principal interaction sites. Site 1 comprises interactions between a unique Kv4 channel N-terminal hydrophobic segment and a hydrophobic pocket formed by displacement of the KChIP H10 helix. Site 2 comprises interactions between a T1 assembly domain loop and the KChIP H2 helix. Functional and biochemical studies indicate that site 1 influences channel trafficking, whereas site 2 affects channel gating, and that calcium binding is intimately linked to KChIP folding and complex formation. Together, the data resolve how Kv4 channels and KChIPs interact and provide a framework for understanding how KChIPs modulate Kv4 function.
An,
Modulation of A-type potassium channels by a family of calcium sensors.
2000, Pubmed,
Xenbase
An,
Modulation of A-type potassium channels by a family of calcium sensors.
2000,
Pubmed
,
Xenbase
Birnbaum,
Structure and function of Kv4-family transient potassium channels.
2004,
Pubmed
Bourne,
Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1).
2001,
Pubmed
Bowlby,
Identification and characterization of small molecule modulators of KChIP/Kv4 function.
2005,
Pubmed
,
Xenbase
Burgoyne,
Neuronal Ca2+-sensor proteins: multitalented regulators of neuronal function.
2004,
Pubmed
Bähring,
Coupling of voltage-dependent potassium channel inactivation and oxidoreductase active site of Kvbeta subunits.
2001,
Pubmed
,
Xenbase
Bähring,
Conserved Kv4 N-terminal domain critical for effects of Kv channel-interacting protein 2.2 on channel expression and gating.
2001,
Pubmed
Bähring,
Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channels.
2001,
Pubmed
Callsen,
Contribution of N- and C-terminal Kv4.2 channel domains to KChIP interaction [corrected].
2005,
Pubmed
Decher,
Novel KChIP2 isoforms increase functional diversity of transient outward potassium currents.
2004,
Pubmed
,
Xenbase
Derewenda,
Entropy and surface engineering in protein crystallization.
2006,
Pubmed
Deschênes,
Regulation of Kv4.3 current by KChIP2 splice variants: a component of native cardiac I(to)?
2002,
Pubmed
Deschênes,
Modulation of Kv4.3 current by accessory subunits.
2002,
Pubmed
Gebauer,
N-type inactivation features of Kv4.2 channel gating.
2004,
Pubmed
Gu,
A conserved domain in axonal targeting of Kv1 (Shaker) voltage-gated potassium channels.
2003,
Pubmed
Guo,
Modulation of Kv4-encoded K(+) currents in the mammalian myocardium by neuronal calcium sensor-1.
2002,
Pubmed
Hasdemir,
Traffic of Kv4 K+ channels mediated by KChIP1 is via a novel post-ER vesicular pathway.
2005,
Pubmed
Hatano,
Functional interaction between KChIP1 and GFP-fused Kv4.3L co-expressed in HEK293 cells.
2002,
Pubmed
Holmqvist,
Elimination of fast inactivation in Kv4 A-type potassium channels by an auxiliary subunit domain.
2002,
Pubmed
,
Xenbase
Jerng,
K+ channel inactivation mediated by the concerted action of the cytoplasmic N- and C-terminal domains.
1997,
Pubmed
,
Xenbase
Kholod,
Novel vectors for co-expression of two proteins in E. coli.
2001,
Pubmed
Kim,
Three-dimensional structure of I(to); Kv4.2-KChIP2 ion channels by electron microscopy at 21 Angstrom resolution.
2004,
Pubmed
Kunjilwar,
KChIP3 rescues the functional expression of Shal channel tetramerization mutants.
2004,
Pubmed
Kuo,
A defect in the Kv channel-interacting protein 2 (KChIP2) gene leads to a complete loss of I(to) and confers susceptibility to ventricular tachycardia.
2001,
Pubmed
Kääb,
Molecular basis of transient outward potassium current downregulation in human heart failure: a decrease in Kv4.3 mRNA correlates with a reduction in current density.
1998,
Pubmed
Levitan,
Signaling protein complexes associated with neuronal ion channels.
2006,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
Minor,
Transmembrane structure of an inwardly rectifying potassium channel.
1999,
Pubmed
Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase
Nakahira,
Selective interaction of voltage-gated K+ channel beta-subunits with alpha-subunits.
1996,
Pubmed
Nakamura,
Different effects of the Ca(2+)-binding protein, KChIP1, on two Kv4 subfamily members, Kv4.1 and Kv4.2.
2001,
Pubmed
,
Xenbase
Nakamura,
A role for frequenin, a Ca2+-binding protein, as a regulator of Kv4 K+-currents.
2001,
Pubmed
,
Xenbase
O'Callaghan,
Residues within the myristoylation motif determine intracellular targeting of the neuronal Ca2+ sensor protein KChIP1 to post-ER transport vesicles and traffic of Kv4 K+ channels.
2003,
Pubmed
Patel,
Regulation of Kv4.3 voltage-dependent gating kinetics by KChIP2 isoforms.
2004,
Pubmed
,
Xenbase
Patel,
Transient outward potassium current, 'Ito', phenotypes in the mammalian left ventricle: underlying molecular, cellular and biophysical mechanisms.
2005,
Pubmed
Patel,
Heterogeneous expression of KChIP2 isoforms in the ferret heart.
2002,
Pubmed
,
Xenbase
Patel,
Elucidating KChIP effects on Kv4.3 inactivation and recovery kinetics with a minimal KChIP2 isoform.
2002,
Pubmed
,
Xenbase
Ren,
Effective association of Kv channel-interacting proteins with Kv4 channel is mediated with their unique core peptide.
2003,
Pubmed
Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase
Rhodes,
KChIPs and Kv4 alpha subunits as integral components of A-type potassium channels in mammalian brain.
2004,
Pubmed
Rosati,
Concordant expression of KChIP2 mRNA, protein and transient outward current throughout the canine ventricle.
2003,
Pubmed
Rosati,
Regulation of ion channel expression.
2004,
Pubmed
Rosati,
Regulation of KChIP2 potassium channel beta subunit gene expression underlies the gradient of transient outward current in canine and human ventricle.
2001,
Pubmed
,
Xenbase
Roux,
Ion conduction and selectivity in K(+) channels.
2005,
Pubmed
Salvador-Recatalà,
A potassium channel (Kv4) cloned from the heart of the tunicate Ciona intestinalis and its modulation by a KChIP subunit.
2006,
Pubmed
Scannevin,
Two N-terminal domains of Kv4 K(+) channels regulate binding to and modulation by KChIP1.
2004,
Pubmed
Shibata,
A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
2003,
Pubmed
Svergun,
Determination of domain structure of proteins from X-ray solution scattering.
2001,
Pubmed
Tombola,
How far will you go to sense voltage?
2005,
Pubmed
Van Petegem,
Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain.
2004,
Pubmed
Wang,
Functionally active t1-t1 interfaces revealed by the accessibility of intracellular thiolate groups in kv4 channels.
2005,
Pubmed
,
Xenbase
Wang,
Voltage-dependent gating rearrangements in the intracellular T1-T1 interface of a K+ channel.
2006,
Pubmed
,
Xenbase
Yang,
Kvbeta subunits increase expression of Kv4.3 channels by interacting with their C termini.
2001,
Pubmed
Zhou,
Structural insights into the functional interaction of KChIP1 with Shal-type K(+) channels.
2004,
Pubmed
