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Nat Methods 2007 Apr 01;44:319-21. doi: 10.1038/nmeth1024.
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Subunit counting in membrane-bound proteins.

Ulbrich MH , Isacoff EY .

The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest. After testing the method with proteins of known stoichiometry expressed in Xenopus laevis oocytes, we resolved the composition of NMDA receptors composed of NR1 and NR3 subunits.

PubMed ID: 17369835
PMC ID: PMC2744285
Article link: Nat Methods
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: grin1 nodal1 nodal3.1

References [+] :
Chatterton, Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. 2002, Pubmed, Xenbase