Science 2008 Jan 25;3195862:469-72. doi: 10.1126/science.1148980.

Centromeric Aurora-B activation requires TD-60, microtubules, and substrate priming phosphorylation.

???displayArticle.abstract???
The chromosome passenger complex (CPC) controls chromosome congression, kinetochore-microtubule attachments, and spindle checkpoint signaling during mitosis. Aurora-B kinase is the catalytic subunit of the CPC. To understand how a single kinase can regulate such diverse events, we have investigated the activation of Aurora-B and describe two distinct activation mechanisms. First, Aurora-B activation in vitro requires two cofactors, telophase disc-60kD (TD-60) and microtubules. TD-60 is critical to localize both the CPC and Haspin kinase activity to centromeres and thus regulates Aurora-B at several levels. Second, Aurora-B substrates can inhibit kinase activation, and this is relieved by phosphorylation of these substrates by the centromeric kinases Plk1 and Haspin. These regulatory mechanisms suggest models for phosphorylation by Aurora-B of centromeric substrates at unaligned chromosomes and merotelic attachments.

???displayArticle.pubmedLink??? 18218899
???displayArticle.link??? Science
???displayArticle.grants??? [+]

Species referenced: Xenopus
Genes referenced: haspin plk1 rcc2
???displayArticle.antibodies??? Aurkb Ab2 H3f3a Ab8 Incenp Ab1 Kif2c Ab1 Kif2c Ab2