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Proc Natl Acad Sci U S A
2008 Jan 08;1051:198-203. doi: 10.1073/pnas.0707948105.
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Ca2+ channel-independent requirement for MAGUK family CACNB4 genes in initiation of zebrafish epiboly.
Ebert AM
,
McAnelly CA
,
Srinivasan A
,
Linker JL
,
Horne WA
,
Garrity DM
.
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CACNB genes encode membrane-associated guanylate kinase (MAGUK) proteins once thought to function exclusively as auxiliary beta subunits in assembly and gating of voltage-gated Ca(2+) channels. Here, we report that zygotic deficiency of zebrafish beta4 protein blocks initiation of epiboly, the first morphogenetic movement of teleost embryos. Reduced beta4 function in the yolk syncytial layer (YSL) leads to abnormal division and dispersal of yolk syncytial nuclei, blastoderm retraction, and death, effects highly similar to microtubule disruption by nocodazole. Epiboly is restored by coinjection of human beta4 cRNA or, surprisingly, by mutant cRNA encoding beta4 subunits incapable of binding to Ca(2+) channel alpha1 subunits. This study defines a YSL-driven zygotic mechanism essential for epiboly initiation and reveals a Ca(2+) channel-independent beta4 protein function potentially involving the cytoskeleton.
Betchaku,
Contact relations, surface activity, and cortical microfilaments of marginal cells of the enveloping layer and of the yolk syncytial and yolk cytoplasmic layers of fundulus before and during epiboly.
1978, Pubmed
Betchaku,
Contact relations, surface activity, and cortical microfilaments of marginal cells of the enveloping layer and of the yolk syncytial and yolk cytoplasmic layers of fundulus before and during epiboly.
1978,
Pubmed
Bruce,
T-box gene eomesodermin and the homeobox-containing Mix/Bix gene mtx2 regulate epiboly movements in the zebrafish.
2005,
Pubmed
Béguin,
Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem.
2001,
Pubmed
,
Xenbase
Chen,
The role of the yolk syncytial layer in germ layer patterning in zebrafish.
2000,
Pubmed
Chen,
Structural basis of the alpha1-beta subunit interaction of voltage-gated Ca2+ channels.
2004,
Pubmed
Cheng,
Organization and function of microfilaments during late epiboly in zebrafish embryos.
2004,
Pubmed
Dosch,
Maternal control of vertebrate development before the midblastula transition: mutants from the zebrafish I.
2004,
Pubmed
Fischer,
Drosophila klarsicht has distinct subcellular localization domains for nuclear envelope and microtubule localization in the eye.
2004,
Pubmed
Fogarty,
The Drosophila maternal-effect mutation grapes causes a metaphase arrest at nuclear cycle 13.
1994,
Pubmed
Golling,
Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development.
2002,
Pubmed
Gonzalez-Gutierrez,
The Src homology 3 domain of the beta-subunit of voltage-gated calcium channels promotes endocytosis via dynamin interaction.
2007,
Pubmed
,
Xenbase
He,
Functional modularity of the beta-subunit of voltage-gated Ca2+ channels.
2007,
Pubmed
Helton,
Alternative splicing of the beta 4 subunit has alpha1 subunit subtype-specific effects on Ca2+ channel gating.
2002,
Pubmed
,
Xenbase
Hidalgo,
Multiplicity of protein interactions and functions of the voltage-gated calcium channel beta-subunit.
2007,
Pubmed
Kane,
The zebrafish epiboly mutants.
1996,
Pubmed
Kimmel,
Stages of embryonic development of the zebrafish.
1995,
Pubmed
,
Xenbase
Köppen,
Coordinated cell-shape changes control epithelial movement in zebrafish and Drosophila.
2006,
Pubmed
Malone,
The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus.
2003,
Pubmed
Mullins,
Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes.
1996,
Pubmed
Nasevicius,
Effective targeted gene 'knockdown' in zebrafish.
2000,
Pubmed
Pragnell,
Calcium channel beta-subunit binds to a conserved motif in the I-II cytoplasmic linker of the alpha 1-subunit.
1994,
Pubmed
Rohde,
Zebrafish gastrulation: cell movements, signals, and mechanisms.
2007,
Pubmed
Sharma,
Role of Fyn kinase in signaling associated with epiboly during zebrafish development.
2005,
Pubmed
Solnica-Krezel,
Microtubule arrays of the zebrafish yolk cell: organization and function during epiboly.
1994,
Pubmed
Solnica-Krezel,
Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish.
1996,
Pubmed
Solnica-Krezel,
Gastrulation in zebrafish -- all just about adhesion?
2006,
Pubmed
Strähle,
Ultraviolet irradiation impairs epiboly in zebrafish embryos: evidence for a microtubule-dependent mechanism of epiboly.
1993,
Pubmed
Thisse,
Structure of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos.
1993,
Pubmed
Tsai,
Role of Yes kinase during early zebrafish development.
2005,
Pubmed
Umeda,
ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation.
2006,
Pubmed
Van Petegem,
Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain.
2004,
Pubmed
Wagner,
Maternal control of development at the midblastula transition and beyond: mutants from the zebrafish II.
2004,
Pubmed
Webster,
Supernova (spno), a new maternal mutant producing variable-sized cleavage nuclei in Drosophila.
1992,
Pubmed
Zalik,
Cell adhesion and the actin cytoskeleton of the enveloping layer in the zebrafish embryo during epiboly.
1999,
Pubmed
