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EMBO J
2008 Feb 20;274:606-17. doi: 10.1038/emboj.2008.9.
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WGEF activates Rho in the Wnt-PCP pathway and controls convergent extension in Xenopus gastrulation.
Tanegashima K
,
Zhao H
,
Dawid IB
.
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The Wnt-PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re-organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide-exchange factors (GEFs), but the identity of the GEF involved in Wnt-PCP-mediated convergent extension is unknown. Here we report the identification of the weak-similarity GEF (WGEF) gene by a microarray-based screen for notochord enriched genes, and show that WGEF is involved in Wnt-regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant-negative Wnt-11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho-associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled-7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam-1, and deletion of the Dishevelled-binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt-PCP pathway that connects Dishevelled to Rho activation.
Asashima,
Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor).
1990, Pubmed,
Xenbase
Asashima,
Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor).
1990,
Pubmed
,
Xenbase
Benard,
Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases.
1999,
Pubmed
Cheyette,
Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation.
2002,
Pubmed
,
Xenbase
Copp,
The genetic basis of mammalian neurulation.
2003,
Pubmed
Crespo,
Phosphotyrosine-dependent activation of Rac-1 GDP/GTP exchange by the vav proto-oncogene product.
1997,
Pubmed
Daggett,
Developmentally restricted actin-regulatory molecules control morphogenetic cell movements in the zebrafish gastrula.
2004,
Pubmed
Djiane,
Role of frizzled 7 in the regulation of convergent extension movements during gastrulation in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Elul,
Cellular mechanism underlying neural convergent extension in Xenopus laevis embryos.
1997,
Pubmed
,
Xenbase
Habas,
Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation.
2003,
Pubmed
,
Xenbase
Habas,
Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel Formin homology protein Daam1.
2001,
Pubmed
,
Xenbase
Hall,
Rho GTPases and the actin cytoskeleton.
1998,
Pubmed
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Keller,
Shaping the vertebrate body plan by polarized embryonic cell movements.
2002,
Pubmed
Keller,
Early embryonic development of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Keller,
Xenopus Gastrulation without a blastocoel roof.
1992,
Pubmed
,
Xenbase
Kim,
Essential role for beta-arrestin 2 in the regulation of Xenopus convergent extension movements.
2007,
Pubmed
,
Xenbase
Kim,
JNK and ROKalpha function in the noncanonical Wnt/RhoA signaling pathway to regulate Xenopus convergent extension movements.
2005,
Pubmed
,
Xenbase
Klein,
Planar cell polarization: an emerging model points in the right direction.
2005,
Pubmed
Kwan,
A microtubule-binding Rho-GEF controls cell morphology during convergent extension of Xenopus laevis.
2005,
Pubmed
,
Xenbase
Liu,
NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio.
1998,
Pubmed
Medina,
Interaction of Frizzled 7 and Dishevelled in Xenopus.
2000,
Pubmed
,
Xenbase
Miyakoshi,
Rho guanine nucleotide exchange factor xNET1 implicated in gastrulation movements during Xenopus development.
2004,
Pubmed
,
Xenbase
Mlodzik,
Planar cell polarization: do the same mechanisms regulate Drosophila tissue polarity and vertebrate gastrulation?
2002,
Pubmed
Nobes,
Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.
1995,
Pubmed
Pannese,
The Xenopus homologue of Otx2 is a maternal homeobox gene that demarcates and specifies anterior body regions.
1995,
Pubmed
,
Xenbase
Park,
Subcellular localization and signaling properties of dishevelled in developing vertebrate embryos.
2005,
Pubmed
,
Xenbase
Park,
Role of PKA as a negative regulator of PCP signaling pathway during Xenopus gastrulation movements.
2006,
Pubmed
,
Xenbase
Ren,
Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton.
1999,
Pubmed
Ren,
Migrating anterior mesoderm cells and intercalating trunk mesoderm cells have distinct responses to Rho and Rac during Xenopus gastrulation.
2006,
Pubmed
,
Xenbase
Rossman,
GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors.
2005,
Pubmed
Sasai,
Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes.
1994,
Pubmed
,
Xenbase
Schmidt,
Guanine nucleotide exchange factors for Rho GTPases: turning on the switch.
2002,
Pubmed
Shamah,
EphA receptors regulate growth cone dynamics through the novel guanine nucleotide exchange factor ephexin.
2001,
Pubmed
Shih,
Cell motility driving mediolateral intercalation in explants of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Smith,
Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction.
1991,
Pubmed
,
Xenbase
Sokol,
Analysis of Dishevelled signalling pathways during Xenopus development.
1996,
Pubmed
,
Xenbase
Tada,
Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway.
2000,
Pubmed
,
Xenbase
Tahinci,
Distinct functions of Rho and Rac are required for convergent extension during Xenopus gastrulation.
2003,
Pubmed
,
Xenbase
Tanegashima,
Xantivin suppresses the activity of EGF-CFC genes to regulate nodal signaling.
2004,
Pubmed
,
Xenbase
Tse,
Identification, expression analysis, genomic organization and cellular location of a novel protein with a RhoGEF domain.
2005,
Pubmed
Tybulewicz,
Vav-family proteins in T-cell signalling.
2005,
Pubmed
Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase
Wallingford,
Convergent extension: the molecular control of polarized cell movement during embryonic development.
2002,
Pubmed
,
Xenbase
Wallingford,
The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity.
2005,
Pubmed
Wang,
WGEF is a novel RhoGEF expressed in intestine, liver, heart, and kidney.
2004,
Pubmed
Wang,
Dishevelled genes mediate a conserved mammalian PCP pathway to regulate convergent extension during neurulation.
2006,
Pubmed
,
Xenbase
Winter,
Drosophila Rho-associated kinase (Drok) links Frizzled-mediated planar cell polarity signaling to the actin cytoskeleton.
2001,
Pubmed
Witzel,
Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane.
2006,
Pubmed
Wünnenberg-Stapleton,
Involvement of the small GTPases XRhoA and XRnd1 in cell adhesion and head formation in early Xenopus development.
1999,
Pubmed
,
Xenbase
von Dassow,
Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene.
1993,
Pubmed
,
Xenbase
