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XB-ART-38178
Dev Biol October 1, 2008; 322 (1): 86-94.
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A p38 MAPK-CREB pathway functions to pattern mesoderm in Xenopus.

Keren A , Keren-Politansky A , Bengal E .


Abstract
Dorsal-ventral patterning is specified by signaling centers secreting antagonizing morphogens that form a signaling gradient. Yet, how morphogen gradient is translated intracellularly into fate decisions remains largely unknown. Here, we report that p38 MAPK and CREB function along the dorsal-ventral axis in mesoderm patterning. We find that the phosphorylated form of CREB (S133) is distributed in a gradient along the dorsal-ventral mesoderm axis and that the p38 MAPK pathway mediates the phosphorylation of CREB. Knockdown of CREB prevents chordin expression and mesoderm dorsalization by the Spemann organizer, whereas ectopic expression of activated CREB-VP16 chimera induces chordin expression and dorsalizes mesoderm. Expression of high levels of p38 activator, MKK6E or CREB-VP16 in embryos converts ventral mesoderm into a dorsal organizing center. p38 MAPK and CREB function downstream of maternal Wnt/beta-catenin and the organizer-specific genes siamois and goosecoid. At low expression levels, MKK6E induces expression of lateral genes without inducing the expression of dorsal genes. Loss of CREB or p38 MAPK activity enables the expansion of the ventral homeobox gene vent1 into the dorsal marginal region, preventing the lateral expression of Xmyf5. Overall, these data indicate that dorsal-ventral mesoderm patterning is regulated by differential p38/CREB activities along the axis.

PubMed ID: 18675264
Article link: Dev Biol


Species referenced: Xenopus laevis
Genes referenced: actl6a chrd.1 creb1 gal.2 grap2 gsc map2k6 mapk1 mapk14 myf5 sia1 ventx1.2 ventx2.2 wnt8a
Morpholinos: creb1 MO1 mapk14 MO1 ventx1.1 MO2 ventx2.1 MO3


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