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FASEB J
2009 Aug 01;238:2568-75. doi: 10.1096/fj.08-128124.
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Origin of the adult intestinal stem cells induced by thyroid hormone in Xenopus laevis.
Ishizuya-Oka A
,
Hasebe T
,
Buchholz DR
,
Kajita M
,
Fu L
,
Shi YB
.
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In the amphibian intestine during metamorphosis, de novo stem cells generate the adult epithelium analogous to the mammalian counterpart. Interestingly, to date the exact origin of these stem cells remains to be determined, making intestinal metamorphosis a unique model to study development of adult organ-specific stem cells. Here, to determine their origin, we made use of transgenic Xenopus tadpoles expressing green fluorescent protein (GFP) for recombinant organ cultures. The larval epithelium separated from the wild-type (Wt) or GFP transgenic (Tg) intestine before metamorphic climax was recombined with homologous and heterologous nonepithelial tissues and was cultivated in the presence of thyroid hormone, the causative agent of metamorphosis. In all kinds of recombinant intestine, adult progenitor cells expressing markers for intestinal stem cells such as sonic hedgehog became detectable and then differentiated into the adult epithelium expressing intestinal fatty acid binding-protein, a marker for absorptive cells. Notably, whenever the epithelium was derived from Tg intestine, both the adult progenitor/stem cells and their differentiated cells expressed GFP, whereas neither of them expressed GFP in the Wt-derived epithelium. Our results provide direct evidence that stem cells that generate the adult intestinal epithelium originate from the larval epithelium, through thyroid hormone-induced dedifferentiation.
Amano,
Isolation of genes involved in intestinal remodeling during anuran metamorphosis.
1998, Pubmed,
Xenbase
Amano,
Isolation of genes involved in intestinal remodeling during anuran metamorphosis.
1998,
Pubmed
,
Xenbase
Amano,
Metamorphosis-associated and region-specific expression of calbindin gene in the posterior intestinal epithelium of Xenopus laevis larva.
1998,
Pubmed
,
Xenbase
Bjerknes,
The stem-cell zone of the small intestinal epithelium. III. Evidence from columnar, enteroendocrine, and mucous cells in the adult mouse.
1981,
Pubmed
Blau,
The evolving concept of a stem cell: entity or function?
2001,
Pubmed
Buchholz,
Transgenic analysis reveals that thyroid hormone receptor is sufficient to mediate the thyroid hormone signal in frog metamorphosis.
2004,
Pubmed
,
Xenbase
Buchholz,
Pairing morphology with gene expression in thyroid hormone-induced intestinal remodeling and identification of a core set of TH-induced genes across tadpole tissues.
2007,
Pubmed
,
Xenbase
Cheng,
Whole population cell kinetics and postnatal development of the mouse intestinal epithelium.
1985,
Pubmed
Fu,
Novel double promoter approach for identification of transgenic animals: A tool for in vivo analysis of gene function and development of gene-based therapies.
2002,
Pubmed
,
Xenbase
Hasebe,
Thyroid hormone-up-regulated hedgehog interacting protein is involved in larval-to-adult intestinal remodeling by regulating sonic hedgehog signaling pathway in Xenopus laevis.
2008,
Pubmed
,
Xenbase
He,
BMP signaling inhibits intestinal stem cell self-renewal through suppression of Wnt-beta-catenin signaling.
2004,
Pubmed
Ishizuya-Oka,
Thyroid hormone-upregulated expression of Musashi-1 is specific for progenitor cells of the adult epithelium during amphibian gastrointestinal remodeling.
2003,
Pubmed
,
Xenbase
Ishizuya-Oka,
Regulation of adult intestinal epithelial stem cell development by thyroid hormone during Xenopus laevis metamorphosis.
2007,
Pubmed
,
Xenbase
Ishizuya-Oka,
Induction of metamorphosis by thyroid hormone in anuran small intestine cultured organotypically in vitro.
1991,
Pubmed
,
Xenbase
Ishizuya-Oka,
Apoptosis and cell proliferation in the Xenopus small intestine during metamorphosis.
1996,
Pubmed
,
Xenbase
Ishizuya-Oka,
Anteroposterior gradient of epithelial transformation during amphibian intestinal remodeling: immunohistochemical detection of intestinal fatty acid-binding protein.
1997,
Pubmed
,
Xenbase
Ishizuya-Oka,
Requirement for matrix metalloproteinase stromelysin-3 in cell migration and apoptosis during tissue remodeling in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Ishizuya-Oka,
Thyroid hormone-induced expression of sonic hedgehog correlates with adult epithelial development during remodeling of the Xenopus stomach and intestine.
2001,
Pubmed
,
Xenbase
Ishizuya-Oka,
Connective tissue is involved in adult epithelial development of the small intestine during anuran metamorphosis in vitro.
1992,
Pubmed
Kayahara,
Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine.
2003,
Pubmed
Kikuyama,
Aspects of amphibian metamorphosis: hormonal control.
1993,
Pubmed
,
Xenbase
Krause,
Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell.
2001,
Pubmed
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Marshall,
Cell specialization in the epithelium of the small intestine of feeding Xenopus laevis tadpoles.
1978,
Pubmed
,
Xenbase
McAvoy,
Cell specialization in the small intestinal epithelium of adult Xenopus laevis: structural aspects.
1978,
Pubmed
,
Xenbase
Mills,
The intestinal stem cell niche: there grows the neighborhood.
2001,
Pubmed
Mukhi,
Remodeling the exocrine pancreas at metamorphosis in Xenopus laevis.
2008,
Pubmed
,
Xenbase
Offield,
The development of Xenopus tropicalis transgenic lines and their use in studying lens developmental timing in living embryos.
2000,
Pubmed
,
Xenbase
Okamoto,
Damaged epithelia regenerated by bone marrow-derived cells in the human gastrointestinal tract.
2002,
Pubmed
Potten,
Identification of a putative intestinal stem cell and early lineage marker; musashi-1.
2003,
Pubmed
Potten,
The intestinal epithelial stem cell: the mucosal governor.
1997,
Pubmed
Potten,
Stem cells in gastrointestinal epithelium: numbers, characteristics and death.
1998,
Pubmed
Schreiber,
Remodeling of the intestine during metamorphosis of Xenopus laevis.
2005,
Pubmed
,
Xenbase
Shi,
Regulation of extracellular matrix remodeling and cell fate determination by matrix metalloproteinase stromelysin-3 during thyroid hormone-dependent post-embryonic development.
2007,
Pubmed
,
Xenbase
Shi,
The earliest changes in gene expression in tadpole intestine induced by thyroid hormone.
1993,
Pubmed
,
Xenbase
Shi,
Thyroid hormone-dependent regulation of the intestinal fatty acid-binding protein gene during amphibian metamorphosis.
1994,
Pubmed
,
Xenbase
Shi,
Biphasic intestinal development in amphibians: embryogenesis and remodeling during metamorphosis.
1996,
Pubmed
,
Xenbase
Shimizu,
Isolation of connective-tissue-specific genes involved in Xenopus intestinal remodeling: thyroid hormone up-regulates Tolloid/BMP-1 expression.
2002,
Pubmed
,
Xenbase
Spradling,
Stem cells find their niche.
2001,
Pubmed
Su,
Thyroid hormone induces apoptosis in primary cell cultures of tadpole intestine: cell type specificity and effects of extracellular matrix.
1997,
Pubmed
,
Xenbase
Suzuki,
Lineage of anuran epidermal basal cells and their differentiation potential in relation to metamorphic skin remodeling.
2002,
Pubmed
,
Xenbase
Tata,
Gene expression during metamorphosis: an ideal model for post-embryonic development.
1993,
Pubmed
Utoh,
Platelet-derived growth factor signaling as a cue of the epithelial-mesenchymal interaction required for anuran skin metamorphosis.
2003,
Pubmed
,
Xenbase
Yoshizato,
Molecular mechanism and evolutional significance of epithelial-mesenchymal interactions in the body- and tail-dependent metamorphic transformation of anuran larval skin.
2007,
Pubmed
Yoshizato,
Biochemistry and cell biology of amphibian metamorphosis with a special emphasis on the mechanism of removal of larval organs.
1989,
Pubmed
de Santa Barbara,
Development and differentiation of the intestinal epithelium.
2003,
Pubmed
