Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2004 Feb 10;1016:1760-3. doi: 10.1073/pnas.0308224100.
Show Gene links
Show Anatomy links
Microtransplantation of functional receptors and channels from the Alzheimer's brain to frog oocytes.
Miledi R
,
Dueñas Z
,
Martinez-Torres A
,
Kawas CH
,
Eusebi F
.
???displayArticle.abstract???
About a decade ago, cell membranes from the electric organ of Torpedo and from the rat brain were transplanted to frog oocytes, which thus acquired functional Torpedo and rat neurotransmitter receptors. Nevertheless, the great potential that this method has for studying human diseases has remained virtually untapped. Here, we show that cell membranes from the postmortem brains of humans that suffered Alzheimer's disease can be microtransplanted to the plasma membrane of Xenopus oocytes. We show also that these postmortem membranes carry neurotransmitter receptors and voltage-operated channels that are still functional, even after they have been kept frozen for many years. This method provides a new and powerful approach to study directly the functional characteristics and structure of receptors, channels, and other membrane proteins of the Alzheimer's brain. This knowledge may help in understanding the basis of Alzheimer's disease and also help in developing new treatments.
Arendt,
Alzheimer's disease as a disorder of mechanisms underlying structural brain self-organization.
2001, Pubmed
Arendt,
Alzheimer's disease as a disorder of mechanisms underlying structural brain self-organization.
2001,
Pubmed
Arispe,
Giant multilevel cation channels formed by Alzheimer disease amyloid beta-protein [A beta P-(1-40)] in bilayer membranes.
1993,
Pubmed
Arispe,
beta-Amyloid Ca(2+)-channel hypothesis for neuronal death in Alzheimer disease.
1994,
Pubmed
Chu,
Quantitative autoradiography of hippocampal GABAB and GABAA receptor changes in Alzheimer's disease.
1987,
Pubmed
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Howell,
Density and pharmacology of alpha5 subunit-containing GABA(A) receptors are preserved in hippocampus of Alzheimer's disease patients.
2000,
Pubmed
Hynd,
Glutamate(NMDA) receptor NR1 subunit mRNA expression in Alzheimer's disease.
2001,
Pubmed
Ikonomovic,
Age-related loss of the AMPA receptor subunits GluR2/3 in the human nucleus basalis of Meynert.
2000,
Pubmed
Ikonomovic,
Distribution of glutamate receptor subunit NMDAR1 in the hippocampus of normal elderly and patients with Alzheimer's disease.
1999,
Pubmed
Kagan,
The channel hypothesis of Alzheimer's disease: current status.
2002,
Pubmed
Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase
Lin,
Amyloid beta protein forms ion channels: implications for Alzheimer's disease pathophysiology.
2001,
Pubmed
Maccioni,
The molecular bases of Alzheimer's disease and other neurodegenerative disorders.
2001,
Pubmed
Marsal,
Incorporation of acetylcholine receptors and Cl- channels in Xenopus oocytes injected with Torpedo electroplaque membranes.
1995,
Pubmed
,
Xenbase
Meyer,
Positron emission tomography measures of benzodiazepine binding in Alzheimer's disease.
1995,
Pubmed
Miledi,
Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes.
2002,
Pubmed
,
Xenbase
Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Mizukami,
Immunohistochemical study of GABA(A) receptor beta2/3 subunits in the hippocampal formation of aged brains with Alzheimer-related neuropathologic changes.
1997,
Pubmed
Mizukami,
GABAA receptor beta 2 and beta 3 subunits mRNA in the hippocampal formation of aged human brain with Alzheimer-related neuropathology.
1998,
Pubmed
Mizukami,
Immunohistochemical study of GABAA receptor alpha1 subunit in the hippocampal formation of aged brains with Alzheimer-related neuropathologic changes.
1998,
Pubmed
Mulugeta,
Loss of muscarinic M4 receptors in hippocampus of Alzheimer patients.
2003,
Pubmed
Ohyama,
Preserved benzodiazepine receptors in Alzheimer's disease measured with C-11 flumazenil PET and I-123 iomazenil SPECT in comparison with CBF.
1999,
Pubmed
Palma,
Expression of human epileptic temporal lobe neurotransmitter receptors in Xenopus oocytes: An innovative approach to study epilepsy.
2002,
Pubmed
,
Xenbase
Ragsdale,
Expressional potency of mRNAs encoding receptors and voltage-activated channels in the postmortem rat brain.
1991,
Pubmed
,
Xenbase
Selkoe,
Alzheimer's disease: genes, proteins, and therapy.
2001,
Pubmed
Selkoe,
Alzheimer's disease is a synaptic failure.
2002,
Pubmed
Small,
Alzheimer's disease and Abeta toxicity: from top to bottom.
2001,
Pubmed
Sze,
N-Methyl-D-aspartate receptor subunit proteins and their phosphorylation status are altered selectively in Alzheimer's disease.
2001,
Pubmed
Umbach,
Mercuric ions are potent noncompetitive antagonists of human brain kainate receptors expressed in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Zilles,
Plasticity and neurotransmitter receptor changes in Alzheimer's disease and experimental cortical infarcts.
1995,
Pubmed
