Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-40413
Dev Biol November 1, 2009; 335 (1): 132-42.
Show Gene links Show Anatomy links

Myosin-X is critical for migratory ability of Xenopus cranial neural crest cells.

Nie S , Kee Y , Bronner-Fraser M .


Abstract
The neural crest is a highly migratory cell population, unique to vertebrates, that forms much of the craniofacial skeleton and peripheral nervous system. In exploring the cell biological basis underlying this behavior, we have identified an unconventional myosin, myosin-X (Myo10) that is required for neural crest migration. Myo10 is highly expressed in both premigratory and migrating cranial neural crest (CNC) cells in Xenopus embryos. Disrupting Myo10 expression using antisense morpholino oligonucleotides leads to impaired neural crest migration and subsequent cartilage formation, but only a slight delay in induction. In vivo grafting experiments reveal that Myo10-depleted CNC cells migrate a shorter distance and fail to segregate into distinct migratory streams. Finally, in vitro cultures and cell dissociation-reaggregation assays suggest that Myo10 may be critical for cell protrusion and cell-cell adhesion. These results demonstrate an essential role for Myo10 in normal cranial neural crest migration and suggest a link to cell-cell interactions and formation of processes.

PubMed ID: 19712673
PMC ID: PMC3991693
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: gal.2 myo10 myo10.2 nog snai2 sox10 sox3 twist1 wnt3a
Morpholinos: myo10.2 MO1 myo10.2 MO3


Article Images: [+] show captions
References [+] :
Alfandari, Integrin alpha5beta1 supports the migration of Xenopus cranial neural crest on fibronectin. 2003, Pubmed, Xenbase