Aldiri I and Vetter ML (2009), Characterization of the expression pattern of t...

XB-ART-40522

Dev Dyn 2009 Dec 01;23812:3185-92. doi: 10.1002/dvdy.22120.

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Characterization of the expression pattern of the PRC2 core subunit Suz12 during embryonic development of Xenopus laevis.

Aldiri I , Vetter ML .

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The Polycomb repressive complex 2 is a multimeric aggregate that mediates silencing of a broad range of genes, and is associated with important biological contexts such as stem cell maintenance and cancer progression. PRC2 mainly trimethylates lysine 27 of histone H3 and is composed of three essential core subunits: EZH2, EED, and SUZ12. The Xenopus orthologs of PRC2 subunits Ezh2 and Eed have been described but Suz12 remained unidentified. Here, we report the cloning of the Xenopus Suz12, and determine its spatiotemporal expression during development. Xsuz12 transcript is provided maternally and continues to be expressed throughout development, particularly in the anterior part of the developing central nervous system. Importantly, comparative analysis of the expression of the PRC2 subunits Xez, Xeed, and Xrbbp4 indicates that their expression largely coincides with Xsuz12 in the nervous system, suggesting that PRC2 may have unexplored functions in the development of the frog central nervous system.

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Species referenced: Xenopus laevis
Genes referenced: eed ezh2 rbbp4 suz12

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References [+] :

Barnett, Xenopus Enhancer of Zeste (XEZ); an anteriorly restricted polycomb gene with a role in neural patterning. 2001, Pubmed, Xenbase

	Figure 3. A-L: Whole mount in situ hybridization analysis of Xsuz12 expression starting from stage 15 (neurula). A, B, C, and G are dorsal views. D, F, and H are anterior views. E, I, G, K, and L are lateral views. G and L are magnifications of the head region as viewed laterally at stage 26 and 34, respectively. Inset in K shows an example of a whole-mount in situ hybridization that was performed with Xsuz12 sense probe. M-O: In situ hybridization analysis performed on traverse sections from the head region at stage 41. M and N sections are at the levels of forebrain and hindbrain, respectively. O is a traverse section in the spinal cord. Stages are indicated at the lower right corner of each image. ot, otic vesicle; ba, branchial arches; e, eye.
	Figure 4. Comparison of Xez and Xeed spatial expression during frog development as assessed by whole-mount in situ hybridization. A B, C, D, E, G: Dorsal views. F, H: Anterior views. I, K, M, P: Lateral views. J, L, N, O: Magnifications of the head region in I, K, M, and P, respectively. ot, otic vesicle; ba, branchial arches; e, eye.
	Figure 5. Expression of Xrbbp4 by whole mount in situ hybridization analysis. A B, C, G: Dorsal views. D, F, H: Anterior views. I, K, M, P: Lateral views. Whole mount in situ hybridization performed with a sense probe is shown as inset in 3K. J and L are magnified head regions shown in I and L, respectively. ot, otic vesicle; ba, branchial arches; e, eye.
	suz12 (SUZ12 polycomb repressive complex 2 subunit) gene expression in Xenopus laevis embryos, NF stage 15, as assayed by in situ hybridization. Dorsal view: anterior up.
	suz12 (suppressor of zeste 12 homolog) gene expression in Xenopus laevis embryos, NF stage 21, as assayed by in situ hybridization. Lateral view: anterior left, dorsal up.
	suz12 (suppressor of zeste 12 homolog) gene expression in Xenopus laevis embryos, NF stage 26, as assayed by in situ hybridization. Lateral view: anterior left, dorsal up.
	suz12 (suppressor of zeste 12 homolog) gene expression in Xenopus laevis embryos, NF stage 34, as assayed by in situ hybridization. Lateral view: anterior right, dorsal up.
	rbbp4 (retinoblastoma binding protein 4) gene expression in Xenopus laevis embryos, NF stage 22, as assayed by in situ hybridization. Lateral view: anterior left, dorsal up.
	ezh2 (enhancer of zeste homolog 2) gene expression in Xenopus laevis embryos, NF stage 34, as assayed by in situ hybridization, lateral view, anterior left, dorsal up.
	eed (embryonic ectoderm development) gene expression in Xenopus laevis embryos, NF stage 34, as assayed by in situ hybridization. Lateral view: anterior left, dorsal up.