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XB-ART-41060
Curr Biol 2010 Feb 09;203:253-8. doi: 10.1016/j.cub.2009.12.021.
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Endocytosis is required for efficient apical constriction during Xenopus gastrulation.



Abstract
Coordinated apical constriction (AC) in epithelial sheets drives tissue invagination [1, 2] and is required for diverse morphogenetic movements such as gastrulation [3], neurulation [4, 5], and organogenesis [6]. We showed previously that actomyosin contractility drives AC in Xenopus laevis bottle cells [7]; however, it remained unclear whether it does so in concert with other processes. Here we report that endocytosis-driven membrane remodeling is required for efficient AC. We found endosomes exclusively in bottle cells in the early gastrula. Disrupting endocytosis with dominant-negative dynamin or rab5 perturbed AC, with a significant decrease in constriction rate late in the process, suggesting that endocytosis operates downstream of actomyosin contractility to remove excess membrane. Additionally, disrupting endocytosis during neurulation inhibits AC in hingepoint cells, resulting in neural tube closure defects. Thus, membrane remodeling during AC could be a general mechanism to achieve efficient invagination in embryos.

PubMed ID: 20096583
PMC ID: PMC3310928
Article link: Curr Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actl6a dnm1l dnm2 pnma2 rab5a
Morpholinos: dnm1l MO1 dnm2 MO1


Article Images: [+] show captions
References [+] :
Balklava, Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic. 2007, Pubmed