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XB-ART-41091
Cell 2010 Feb 05;1403:349-59. doi: 10.1016/j.cell.2009.12.049.
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Treslin collaborates with TopBP1 in triggering the initiation of DNA replication.

Kumagai A , Shevchenko A , Shevchenko A , Dunphy WG .


Abstract
TopBP1 has important roles in both DNA replication and checkpoint regulation in vertebrates. We have identified a protein called Treslin that associates with TopBP1 in Xenopus egg extracts. Depletion of Treslin from egg extracts strongly inhibits chromosomal DNA replication. Binding of Treslin to chromatin in egg extracts occurs independently of TopBP1. However, loading of the initiator protein Cdc45 onto chromatin cannot take place in the absence of Treslin. Prior to the initiation of DNA replication, Treslin associates with TopBP1 in a Cdk2-dependent manner. Ablation of Treslin from human cells also strongly inhibits DNA replication. Taken together, these results indicate that Treslin and TopBP1 collaborate in the Cdk2-mediated loading of Cdc45 onto replication origins. Thus, Treslin regulates a pivotal step in the initiation of DNA replication in vertebrates.

PubMed ID: 20116089
PMC ID: PMC2857569
Article link: Cell
Grant support: [+]

Species referenced: Xenopus
Genes referenced: atr cdc45 cdk2 chek1 gmnn h2ax h2ax kit orc2 ticrr topbp1 znrd2


Article Images: [+] show captions
References [+] :
Bell, DNA replication in eukaryotic cells. 2002, Pubmed