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SGK1 increases Na,K-ATP cell-surface expression and function in Xenopus laevis oocytes.
Zecevic M
,
Heitzmann D
,
Camargo SM
,
Verrey F
.
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The Na(+)-retaining hormone aldosterone increases the cell-surface expression of the luminal epithelial sodium channel (ENaC) and the basolateral Na(+) pump (Na,K-ATPase) in aldosterone-sensitive distalnephron cells in a coordinated fashion. To address the question of whether aldosterone-induced serum and glucocorticoid-regulated kinase-1 (SGK1) might be involved in mediating this regulation of Na,K-ATPase subcellular localization, similar to that of the epithelial Na(+) channel (ENaC), we co-expressed the Na,K-ATPase (rat alpha 1- and Xenopus laevis beta 1-subunits) and Xenopus SGK1 in Xenopus oocytes. Measurements of the Na(+) pump current showed that wild-type SGK1 increases the function of exogenous Na,K-ATPase at the surface of Xenopus oocytes. This appeared to be secondary to an increase in Na,K-ATPase cell-surface expression as visualized by Western blotting of surface-biotinylated proteins. In contrast, the functional surface expression of two other exogenous transporters, the heterodimeric amino acid transporter LAT1-4F2hc and the Na(+)/phosphate cotransporter NaPi-IIa, was not increased by SGK1 co-expression. The total pool of exogenous Na,K-ATPase was increased by the co-expression of SGK1, and similarly also by ENaC co-expression. This latter effect depended on the [Na(+)] of the buffer and was not additive to that of SGK1. When the total Na,K-ATPase was increased by ENaC co-expression, SGK1 still increased Na,K-ATPase cell-surface expression. These observations in Xenopus oocytes suggest the possibility that SGK1 induction and/or activation could participate in the coordinated regulation of Na,K-ATPase and ENaC cell-surface expression in the aldosterone-sensitive distalnephron.
Alvarez de la Rosa,
Role of SGK in hormonal regulation of epithelial sodium channel in A6 cells.
2003, Pubmed,
Xenbase
Alvarez de la Rosa,
Role of SGK in hormonal regulation of epithelial sodium channel in A6 cells.
2003,
Pubmed
,
Xenbase
Alvarez de la Rosa,
The serum and glucocorticoid kinase sgk increases the abundance of epithelial sodium channels in the plasma membrane of Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Auberson,
Epithelial Na+ channel mutants causing Liddle's syndrome retain ability to respond to aldosterone and vasopressin.
2003,
Pubmed
Boehmer,
Serum and glucocorticoid inducible kinases in the regulation of the cardiac sodium channel SCN5A.
2003,
Pubmed
,
Xenbase
Bowen,
Pretranslational regulation of Na-K-ATPase in cultured canine kidney cells by low K+.
1987,
Pubmed
Carranza,
Protein kinase C-dependent phosphorylation of Na(+)-K(+)-ATPase alpha-subunit in rat kidney cortical tubules.
1996,
Pubmed
Chen,
Epithelial sodium channel regulated by aldosterone-induced protein sgk.
1999,
Pubmed
,
Xenbase
Coppi,
Ubiquitination of Na,K-ATPase alpha1 and alpha2 subunits.
1997,
Pubmed
Crambert,
FXYD proteins: new tissue-specific regulators of the ubiquitous Na,K-ATPase.
2003,
Pubmed
Dahlmann,
Mineralocorticoid regulation of epithelial Na+ channels is maintained in a mouse model of Liddle's syndrome.
2003,
Pubmed
Debonneville,
Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression.
2001,
Pubmed
,
Xenbase
Embark,
Regulation of KCNE1-dependent K(+) current by the serum and glucocorticoid-inducible kinase (SGK) isoforms.
2003,
Pubmed
,
Xenbase
Faletti,
sgk: an essential convergence point for peptide and steroid hormone regulation of ENaC-mediated Na+ transport.
2002,
Pubmed
,
Xenbase
Firsov,
Cell surface expression of the epithelial Na channel and a mutant causing Liddle syndrome: a quantitative approach.
1996,
Pubmed
,
Xenbase
Gamper,
K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK.
2002,
Pubmed
,
Xenbase
Helms,
Hormone-regulated transepithelial Na+ transport in mammalian CCD cells requires SGK1 expression.
2003,
Pubmed
Jaisser,
Modulation of the Na,K-pump function by beta subunit isoforms.
1994,
Pubmed
,
Xenbase
Loffing,
Aldosterone induces rapid apical translocation of ENaC in early portion of renal collecting system: possible role of SGK.
2001,
Pubmed
,
Xenbase
Mastroberardino,
Ras pathway activates epithelial Na+ channel and decreases its surface expression in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Náray-Fejes-Tóth,
sgk is an aldosterone-induced kinase in the renal collecting duct. Effects on epithelial na+ channels.
1999,
Pubmed
,
Xenbase
Park,
Serum and glucocorticoid-inducible kinase (SGK) is a target of the PI 3-kinase-stimulated signaling pathway.
1999,
Pubmed
Pfeiffer,
Regulation of Na+ pump function by aldosterone is alpha-subunit isoform specific.
1999,
Pubmed
,
Xenbase
Puoti,
The highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells.
1995,
Pubmed
,
Xenbase
Rossier,
LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine.
1999,
Pubmed
,
Xenbase
Setiawan,
Stimulation of Xenopus oocyte Na(+),K(+)ATPase by the serum and glucocorticoid-dependent kinase sgk1.
2002,
Pubmed
,
Xenbase
Shull,
Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.
1986,
Pubmed
Snyder,
Serum and glucocorticoid-regulated kinase modulates Nedd4-2-mediated inhibition of the epithelial Na+ channel.
2002,
Pubmed
Summa,
Short term effect of aldosterone on Na,K-ATPase cell surface expression in kidney collecting duct cells.
2001,
Pubmed
Verrey,
Corticosteroid regulation of renal Na,K-ATPase.
1996,
Pubmed
Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
Pubmed
,
Xenbase
Wagner,
Effects of the serine/threonine kinase SGK1 on the epithelial Na(+) channel (ENaC) and CFTR: implications for cystic fibrosis.
2001,
Pubmed
,
Xenbase
Wagner,
The heterodimeric amino acid transporter 4F2hc/LAT1 is associated in Xenopus oocytes with a non-selective cation channel that is regulated by the serine/threonine kinase sgk-1.
2000,
Pubmed
,
Xenbase
Werner,
Expression of renal transport systems for inorganic phosphate and sulfate in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Yoo,
Cell surface expression of the ROMK (Kir 1.1) channel is regulated by the aldosterone-induced kinase, SGK-1, and protein kinase A.
2003,
Pubmed
,
Xenbase
Yun,
Glucocorticoid activation of Na(+)/H(+) exchanger isoform 3 revisited. The roles of SGK1 and NHERF2.
2002,
Pubmed
Yun,
The serum and glucocorticoid-inducible kinase SGK1 and the Na+/H+ exchange regulating factor NHERF2 synergize to stimulate the renal outer medullary K+ channel ROMK1.
2002,
Pubmed
,
Xenbase
