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J Biol Chem
2010 Oct 29;28544:33584-8. doi: 10.1074/jbc.C110.146381.
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Polycystin-2 activity is controlled by transcriptional coactivator with PDZ binding motif and PALS1-associated tight junction protein.
Duning K
,
Rosenbusch D
,
Schlüter MA
,
Tian Y
,
Kunzelmann K
,
Meyer N
,
Schulze U
,
Markoff A
,
Pavenstädt H
,
Weide T
.
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Autosomal dominant polycystic kidney disease (ADPKD) is the most frequent monogenic cause of kidney failure, characterized by the development of renal cysts. ADPKD is caused by mutations of the polycystin-1 (PC1) or polycystin-2 (PC2) genes. PC2 encodes a Ca(2+)-permeable cation channel, and its dysfunction has been implicated in cyst development. The transcriptional coactivator with PDZ binding motif (TAZ) is required for the integrity of renal cilia. Its absence results in the development of renal cysts in a knock-out mouse model. TAZ directly interacts with PC2, and it has been suggested that another yet unidentified PDZ domain protein may be involved in the TAZ/PC2 interaction. Here we describe a novel interaction of TAZ with the multi-PDZ-containing PALS1-associated tight junction protein (PATJ). TAZ interacts with both the N-terminal PDZ domains 1-3 and the C-terminal PDZ domains 8-10 of PATJ, suggesting two distinct TAZ binding domains. We also show that the C terminus of PC2 strongly interacts with PDZ domains 8-10 and to a weaker extent with PDZ domains 1-3 of PATJ. Finally, we demonstrate that both TAZ and PATJ impair PC2 channel activity when co-expressed with PC2 in oocytes of Xenopus laevis. These results implicate TAZ and PATJ as novel regulatory elements of the PC2 channel and might thus be involved in ADPKD pathology.
Benzing,
Wnt signaling in polycystic kidney disease.
2007, Pubmed
Benzing,
Wnt signaling in polycystic kidney disease.
2007,
Pubmed
Brereton,
Evidence that the TRP-1 protein is unlikely to account for store-operated Ca2+ inflow in Xenopus laevis oocytes.
2000,
Pubmed
,
Xenbase
Chan,
A role for TAZ in migration, invasion, and tumorigenesis of breast cancer cells.
2008,
Pubmed
Chan,
TEADs mediate nuclear retention of TAZ to promote oncogenic transformation.
2009,
Pubmed
Colwill,
Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites.
2006,
Pubmed
Delous,
Nephrocystin-1 and nephrocystin-4 are required for epithelial morphogenesis and associate with PALS1/PATJ and Par6.
2009,
Pubmed
Duning,
KIBRA modulates directional migration of podocytes.
2008,
Pubmed
Ernkvist,
The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells.
2009,
Pubmed
Fan,
Polarity proteins control ciliogenesis via kinesin motor interactions.
2004,
Pubmed
Fliegauf,
When cilia go bad: cilia defects and ciliopathies.
2007,
Pubmed
Fu,
The subcellular localization of TRPP2 modulates its function.
2008,
Pubmed
Hackmann,
A splice form of polycystin-2, lacking exon 7, does not interact with polycystin-1.
2005,
Pubmed
Hamaratoglu,
The Hippo tumor-suppressor pathway regulates apical-domain size in parallel to tissue growth.
2009,
Pubmed
Hanaoka,
Co-assembly of polycystin-1 and -2 produces unique cation-permeable currents.
,
Pubmed
Harris,
Polycystic kidney disease.
2009,
Pubmed
Hong,
TAZ: a beta-catenin-like molecule that regulates mesenchymal stem cell differentiation.
2006,
Pubmed
Hossain,
Glomerulocystic kidney disease in mice with a targeted inactivation of Wwtr1.
2007,
Pubmed
Houslay,
Disrupting specific PDZ domain-mediated interactions for therapeutic benefit.
2009,
Pubmed
Huber,
The transient receptor potential protein (Trp), a putative store-operated Ca2+ channel essential for phosphoinositide-mediated photoreception, forms a signaling complex with NorpA, InaC and InaD.
1996,
Pubmed
Kanai,
TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins.
2000,
Pubmed
Köttgen,
TRPP2 and TRPV4 form a polymodal sensory channel complex.
2008,
Pubmed
Lee,
Kidney claudin-19: localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease.
2006,
Pubmed
Lei,
TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway.
2008,
Pubmed
Li,
TRP and the PDZ protein, INAD, form the core complex required for retention of the signalplex in Drosophila photoreceptor cells.
2000,
Pubmed
Makita,
Multiple renal cysts, urinary concentration defects, and pulmonary emphysematous changes in mice lacking TAZ.
2008,
Pubmed
Murakami,
Transcriptional activity of Pax3 is co-activated by TAZ.
2006,
Pubmed
Nauli,
Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells.
2003,
Pubmed
Nauli,
Polycystins and mechanosensation in renal and nodal cilia.
2004,
Pubmed
Nauli,
Endothelial cilia are fluid shear sensors that regulate calcium signaling and nitric oxide production through polycystin-1.
2008,
Pubmed
Peng,
Anchoring TRP to the INAD macromolecular complex requires the last 14 residues in its carboxyl terminus.
2008,
Pubmed
Schlüter,
Apical lumen formation in renal epithelia.
2009,
Pubmed
Sfakianos,
Par3 functions in the biogenesis of the primary cilium in polarized epithelial cells.
2007,
Pubmed
Shin,
PATJ regulates tight junction formation and polarity in mammalian epithelial cells.
2005,
Pubmed
Shin,
PATJ regulates directional migration of mammalian epithelial cells.
2007,
Pubmed
Stiffler,
PDZ domain binding selectivity is optimized across the mouse proteome.
2007,
Pubmed
Streets,
Homophilic and heterophilic polycystin 1 interactions regulate E-cadherin recruitment and junction assembly in MDCK cells.
2009,
Pubmed
Stumpf,
IADS, a decomposition product of DIDS activates a cation conductance in Xenopus oocytes and human erythrocytes: new compound for the diagnosis of cystic fibrosis.
2006,
Pubmed
,
Xenbase
Thorneloe,
N-((1S)-1-{[4-((2S)-2-{[(2,4-dichlorophenyl)sulfonyl]amino}-3-hydroxypropanoyl)-1-piperazinyl]carbonyl}-3-methylbutyl)-1-benzothiophene-2-carboxamide (GSK1016790A), a novel and potent transient receptor potential vanilloid 4 channel agonist induces urinary bladder contraction and hyperactivity: Part I.
2008,
Pubmed
Tian,
TAZ promotes PC2 degradation through a SCFbeta-Trcp E3 ligase complex.
2007,
Pubmed
Treharne,
Protein kinase CK2, cystic fibrosis transmembrane conductance regulator, and the deltaF508 mutation: F508 deletion disrupts a kinase-binding site.
2007,
Pubmed
,
Xenbase
Tsiokas,
Specific association of the gene product of PKD2 with the TRPC1 channel.
1999,
Pubmed
Tsunoda,
A multivalent PDZ-domain protein assembles signalling complexes in a G-protein-coupled cascade.
1997,
Pubmed
Varelas,
The Hippo pathway regulates Wnt/beta-catenin signaling.
2010,
Pubmed
Wang,
YAP, TAZ, and Yorkie: a conserved family of signal-responsive transcriptional coregulators in animal development and human disease.
2009,
Pubmed
Wilson,
Cystic disease of the kidney.
2007,
Pubmed
Zhao,
The Hippo-YAP pathway: new connections between regulation of organ size and cancer.
2008,
Pubmed
