Hernandez L , Roux KJ , Wong ES , Mounkes LC , Mutalif R , Navasankari R , Rai B , Cool S , Jeong JW , Wang H , Lee HS , Kozlov S , Grunert M , Keeble T , Jones CM , Meta MD , Young SG , Daar IO , Burke B , Perantoni AO , Stewart CL .

R01HD057873 NICHD NIH HHS , Z01 BC005093-29 NCI NIH HHS , R01 AG035626-09 NIA NIH HHS , R01 AG035626 NIA NIH HHS , Z01 BC005093-29 Intramural NIH HHS, R01 HD057873 NICHD NIH HHS

             fibroblast proliferation
             canonical Wnt signaling pathway

Figure 5. δ9 and δ50 Lamin Inhibit Wnt-Mediated Transcription(A) TOPflash/FOPflash luciferase reporter assays with WT (BC), δ9, δ50 lamins, or their SSIM variants.(B) Gst-Tcf pull-down of nuclear β-catenin levels.(C) Injection of Xwnt3a mRNA into Xenopus embryos, alone or in the presence of each LMNA construct shown in (A) and (B). Lower panel, western analysis shows amount of protein derived from the different LMNA mRNAs injected.Error bars are SEM.

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