Methods Mol Biol 2011 Jan 01;770:571-8. doi: 10.1007/978-1-61779-210-6_23.

Keeping two animal systems in one lab - a frog plus fish case study.

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For two decades, my lab has been studying development using two vertebrate animals, the frog Xenopus and the zebrafish, Danio. This has been both productive and challenging. The initial rationale for the choice was to compare the same process in two species, as a means to find commonalities that may carry through all vertebrates. As time progressed, however, each species has become exploited for its specific attributes, more than for comparative studies. Maintaining two species simultaneously has been challenging, as has the division of research between the two and making sure that lab members know both systems well enough to communicate productively. Other significant issues concern funding for disparate research, figuring out how to make contributions to both fish and frog communities, and being accepted as a member of two communities. I discuss whether this dual allegiance has been a good idea.

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