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Lab Anim Res 2011 Jun 01;272:109-16. doi: 10.5625/lar.2011.27.2.109.
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Isolation and Expression Profile of the Ca-Activated Chloride Channel-like Membrane Protein 6 Gene in Xenopus laevis.

Lee RM , Ryu RH , Jeong SW , Oh SJ , Huang H , Han JS , Lee CH , Lee CJ , Jan LY , Jeong SM .

To clone the first anion channel from Xenopus laevis (X. laevis), we isolated a calcium-activated chloride channel (CLCA)-like membrane protein 6 gene (CMP6) in X. laevis. As a first step in gene isolation, an expressed sequence tags database was screened to find the partial cDNA fragment. A putative partial cDNA sequence was obtained by comparison with rat CLCAs identified in our laboratory. First stranded cDNA was synthesized by reverse transcription polymerase-chain reaction (RT-PCR) using a specific primer designed for the target cDNA. Repeating the 5'' and 3'' rapid amplification of cDNA ends, full-length cDNA was constructed from the cDNA pool. The full-length CMP6 cDNA completed via 5''- and 3''-RACE was 2,940 bp long and had an open reading frame (ORF) of 940 amino acids. The predicted 940 polypeptides have four major transmembrane domains and showed about 50% identity with that of rat brain CLCAs in our previously published data. Semi-quantification analysis revealed that CMP6 was most abundantly expressed in small intestine, colon and liver. However, all tissues except small intestine, colon and liver had undetectable levels. This result became more credible after we did real-time PCR quantification for the target gene. In view of all CLCA studies focused on human or murine channels, this finding suggests a hypothetical protein as an ion channel, an X. laevis CLCA.

PubMed ID: 21826170
PMC ID: PMC3146003
Article link: Lab Anim Res

Species referenced: Xenopus laevis
Genes referenced: actl6a clca1.1 clca1.2 clca1.3 clca2 clca3p clca4.1 clca4.2 cyp26a1

Article Images: [+] show captions
References [+] :
Agnel, Identification of three novel members of the calcium-dependent chloride channel (CaCC) family predominantly expressed in the digestive tract and trachea. 1999, Pubmed