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XB-ART-44394
CSH Protoc 2007 Jun 01;2007:pdb.top10. doi: 10.1101/pdb.top10.
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Microdissection: Explant and Transplant Assays in Xenopus laevis.

Sive HL , Grainger RM , Harland RM .


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INTRODUCTIONWhen analyzing any developmental process, important questions are those relating to the formation of different cell types. What makes a cell decide to become a particular cell type? What cell interactions are involved in this decision? The "commitment" of cells to a particular lineage is also called "specification" or "determination." Analysis of the cell interactions, or inductions, required for tissue-type determination is a critical step in the identification of the genes that control the process. Lineage commitment and inductive interactions can be assessed by explant or transplant assays with embryos from Xenopus laevis.

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