Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
RNA
2012 Jun 01;186:1186-96. doi: 10.1261/rna.031369.111.
Show Gene links
Show Anatomy links
Identification of the human PMR1 mRNA endonuclease as an alternatively processed product of the gene for peroxidasin-like protein.
Gu SQ
,
Bakthavachalu B
,
Han J
,
Patil DP
,
Otsuka Y
,
Guda C
,
Schoenberg DR
.
???displayArticle.abstract???
The PMR1 endonuclease was discovered in Xenopus liver and identified as a member of the large and diverse peroxidase gene family. The peroxidase genes arose from multiple duplication and rearrangement events, and their high degree of sequence similarity confounded attempts to identify human PMR1. The functioning of PMR1 in mRNA decay depends on the phosphorylation of a tyrosine in the C-terminal polysome targeting domain by c-Src. The sequences of regions that are required for c-Src binding and phosphorylation of Xenopus PMR1 were used to inform a bioinformatics search that identified two related genes as potential candidates for human PMR1: peroxidasin homolog (PXDN) and peroxidasin homolog-like (PXDNL) protein. Although each of these genes is predicted to encode a large, multidomain membrane-bound peroxidase, alternative splicing of PXDNL pre-mRNA yields a transcript whose predicted product is a 57-kDa protein with 42% sequence identity to Xenopus PMR1. Results presented here confirm the existence of the predicted 57-kDa protein, show this is the only form of PXDNL detected in any of the human cell lines examined, and confirm its identity as human PMR1. Like the Xenopus protein, human PMR1 binds to c-Src, is tyrosine phosphorylated, sediments on polysomes, and catalyzes the selective decay of a PMR1 substrate mRNA. Importantly, the expression of human PMR1 stimulates cell motility in a manner similar to that of the Xenopus PMR1 expressed in human cells, thus providing definitive evidence linking endonuclease decay to the regulation of cell motility.
Affymetrix ENCODE Transcriptome Project,
Post-transcriptional processing generates a diversity of 5'-modified long and short RNAs.
2009, Pubmed
Affymetrix ENCODE Transcriptome Project,
Post-transcriptional processing generates a diversity of 5'-modified long and short RNAs.
2009,
Pubmed
Bailey,
MEME SUITE: tools for motif discovery and searching.
2009,
Pubmed
Barnes,
Identification of Apurinic/apyrimidinic endonuclease 1 (APE1) as the endoribonuclease that cleaves c-myc mRNA.
2009,
Pubmed
Cheng,
Identification and characterization of VPO1, a new animal heme-containing peroxidase.
2008,
Pubmed
Chernokalskaya,
A polysomal ribonuclease involved in the destabilization of albumin mRNA is a novel member of the peroxidase gene family.
1998,
Pubmed
,
Xenbase
Cunningham,
Polysomal ribonuclease 1 exists in a latent form on polysomes prior to estrogen activation of mRNA decay.
2001,
Pubmed
,
Xenbase
Dompenciel,
Purification and characterization of an estrogen-regulated Xenopus liver polysomal nuclease involved in the selective destabilization of albumin mRNA.
1995,
Pubmed
,
Xenbase
Eberle,
SMG6 promotes endonucleolytic cleavage of nonsense mRNA in human cells.
2009,
Pubmed
Han,
IRE1alpha kinase activation modes control alternate endoribonuclease outputs to determine divergent cell fates.
2009,
Pubmed
,
Xenbase
Hollien,
Regulated Ire1-dependent decay of messenger RNAs in mammalian cells.
2009,
Pubmed
Horikoshi,
Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene.
1999,
Pubmed
Huang,
Defining the specificity space of the human SRC homology 2 domain.
2008,
Pubmed
Huntzinger,
SMG6 is the catalytic endonuclease that cleaves mRNAs containing nonsense codons in metazoan.
2008,
Pubmed
Kapranov,
From transcription start site to cell biology.
2009,
Pubmed
Karginov,
Diverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases.
2010,
Pubmed
Matsushita,
Zc3h12a is an RNase essential for controlling immune responses by regulating mRNA decay.
2009,
Pubmed
Mercer,
Regulated post-transcriptional RNA cleavage diversifies the eukaryotic transcriptome.
2010,
Pubmed
Mizgalska,
Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1beta mRNA.
2009,
Pubmed
Murray,
A+U-rich instability elements differentially activate 5'-3' and 3'-5' mRNA decay.
2007,
Pubmed
Nechama,
KSRP-PMR1-exosome association determines parathyroid hormone mRNA levels and stability in transfected cells.
2009,
Pubmed
Parker,
P bodies and the control of mRNA translation and degradation.
2007,
Pubmed
Pastori,
Estrogen-induced ribonuclease activity in Xenopus liver.
1991,
Pubmed
,
Xenbase
Peng,
c-Src activates endonuclease-mediated mRNA decay.
2007,
Pubmed
,
Xenbase
Peng,
The cytoskeleton-associated Ena/VASP proteins are unanticipated partners of the PMR1 mRNA endonuclease.
2009,
Pubmed
,
Xenbase
Plessy,
Linking promoters to functional transcripts in small samples with nanoCAGE and CAGEscan.
2010,
Pubmed
Péterfi,
Peroxidasin is secreted and incorporated into the extracellular matrix of myofibroblasts and fibrotic kidney.
2009,
Pubmed
Schoenberg,
Regulation of cytoplasmic mRNA decay.
2012,
Pubmed
Schoenberg,
Re-capping the message.
2009,
Pubmed
Schoenberg,
Mechanisms of endonuclease-mediated mRNA decay.
2011,
Pubmed
Suzuki,
MCPIP1 ribonuclease antagonizes dicer and terminates microRNA biogenesis through precursor microRNA degradation.
2011,
Pubmed
Tagwerker,
A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking.
2006,
Pubmed
Tomecki,
Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.
2010,
Pubmed
Yang,
Endonuclease-mediated mRNA decay involves the selective targeting of PMR1 to polyribosome-bound substrate mRNA.
2004,
Pubmed
Yang,
Endonuclease-mediated mRNA decay requires tyrosine phosphorylation of polysomal ribonuclease 1 (PMR1) for the targeting and degradation of polyribosome-bound substrate mRNA.
2004,
Pubmed
,
Xenbase
Zamocky,
The peroxidase-cyclooxygenase superfamily: Reconstructed evolution of critical enzymes of the innate immune system.
2008,
Pubmed
