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Biol Open 2012 Aug 15;18:731-8. doi: 10.1242/bio.2012968.
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The endocytic adapter E-Syt2 recruits the p21 GTPase activated kinase PAK1 to mediate actin dynamics and FGF signalling.

Jean S , Tremblay MG , Herdman C , Guillou F , Moss T .

Fibroblast growth factor (FGF) signalling plays an essential role in early vertebrate development. However, the response to FGF requires endocytosis of the activated FGF receptor (FGFR) that is in part dependent on remodelling of the actin cytoskeleton. Recently we showed that the extended synaptotagmin family plasma membrane protein, E-Syt2, is an essential endocytic adapter for FGFR1. Here we show E-Syt2 is also an interaction partner for the p21-GTPase Activated Kinase PAK1. The phospholipid binding C2C domain of E-Syt2 specifically binds a site adjacent to the CRIB/GBD of PAK1. PAK1 and E-Syt2 selectively complex with FGFR1 and functionally cooperate in the FGF signalling. E-Syt2 binding suppresses actin polymerization and inhibits the activation of PAK1 by the GTPases Cdc42 and Rac. Interestingly, the E-Syt2 binding site on PAK1 extensively overlaps a site recently suggested to bind phospholipids. Our data suggest that PAK1 interacts with phospholipid membrane domains via E-Syt2, where it may cooperate in the E-Syt2-dependent endocytosis of activated FGFR1 by modulating cortical actin stability.

PubMed ID: 23213466
PMC ID: PMC3507230
Article link: Biol Open

Species referenced: Xenopus
Genes referenced: acss2.2 actl6a agxt akt1 cdc42 cdkn1a esyt2 fgf2 fgfr1 mbp myc nsg1 odc1 pak1 rac1 syt2 tbx2 tbxt tp53
Morpholinos: esyt2 MO1 esyt2 MO2 esyt2 MO3 esyt2 MO4 esyt2 MO5

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References [+] :
Benink, Concentric zones of active RhoA and Cdc42 around single cell wounds. 2005, Pubmed, Xenbase