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Am J Physiol Renal Physiol
2013 Apr 01;3047:F930-7. doi: 10.1152/ajprenal.00638.2012.
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ENaC is regulated by natriuretic peptide receptor-dependent cGMP signaling.
Guo LJ
,
Alli AA
,
Eaton DC
,
Bao HF
.
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Epithelial sodium channels (ENaCs) located at the apical membrane of polarized epithelial cells are regulated by the second messenger guanosine 3',5'-cyclic monophosphate (cGMP). The mechanism for this regulation has not been completely characterized. Guanylyl cyclases synthesize cGMP in response to various intracellular and extracellular signals. We investigated the regulation of ENaC activity by natriuretic peptide-dependent activation of guanylyl cyclases in Xenopus 2F3 cells. Confocal microscopy studies show natriuretic peptide receptors (NPRs), including those coupled to guanylyl cyclases, are expressed at the apical membrane of 2F3 cells. Single-channel patch-clamp studies using 2F3 cells revealed that atrial natriuretic peptide (ANP) or 8-(4-chlorophenylthio)-cGMP, but not C-type natriuretic peptide or cANP, decreased the open probability of ENaC. This suggests that NPR-A, but not NPR-B or NPR-C, is involved in the natriuretic peptide-mediated regulation of ENaC activity. Also, it is likely that a signaling pathway involving cGMP and nitric oxide (NO) are involved in this mechanism, since inhibitors of soluble guanylyl cyclase, protein kinase G, inducible NO synthase, or an NO scavenger blocked or reduced the effect of ANP on ENaC activity.
Alli,
Phosphatidylinositol phosphate-dependent regulation of Xenopus ENaC by MARCKS protein.
2012, Pubmed,
Xenbase
Alli,
Phosphatidylinositol phosphate-dependent regulation of Xenopus ENaC by MARCKS protein.
2012,
Pubmed
,
Xenbase
Alli,
Molecular approaches to examine the phosphorylation state of the C type natriuretic peptide receptor.
2010,
Pubmed
Alli,
The C type natriuretic peptide receptor tethers AHNAK1 at the plasma membrane to potentiate arachidonic acid-induced calcium mobilization.
2009,
Pubmed
Anand-Srivastava,
Atrial natriuretic factor receptors and signal transduction mechanisms.
1993,
Pubmed
Anand-Srivastava,
Natriuretic peptide receptor-C signaling and regulation.
2005,
Pubmed
Ando,
Atrial natriuretic peptide in human urine.
1988,
Pubmed
Burgess,
C-type natriuretic peptide receptor expression in pancreatic alpha cells.
2009,
Pubmed
Butterworth,
cAMP-sensitive endocytic trafficking in A6 epithelia.
2001,
Pubmed
Das,
Stimulation by cGMP of apical Na channels and cation channels in toad urinary bladder.
1991,
Pubmed
Eichelbaum,
Four cardiac hormones eliminate up to 82% of human medullary thyroid carcinoma cells within 24 hours.
2006,
Pubmed
Eichelbaum,
Cardiac and kidney hormones cure up to 86% of human small-cell lung cancers in mice.
2008,
Pubmed
Gambaryan,
Expression of type II cGMP-dependent protein kinase in rat kidney is regulated by dehydration and correlated with renin gene expression.
1996,
Pubmed
Gower,
Four peptides decrease human colon adenocarcinoma cell number and DNA synthesis via cyclic GMP.
2005,
Pubmed
Helms,
Role of SGK1 in nitric oxide inhibition of ENaC in Na+-transporting epithelia.
2005,
Pubmed
,
Xenbase
Hoenderop,
Atrial natriuretic peptide-stimulated Ca2+ reabsorption in rabbit kidney requires membrane-targeted, cGMP-dependent protein kinase type II.
1999,
Pubmed
Inoue,
Physiological effects of vasopressin and atrial natriuretic peptide in the collecting duct.
2001,
Pubmed
Jain,
Nitric oxide inhibits lung sodium transport through a cGMP-mediated inhibition of epithelial cation channels.
1998,
Pubmed
Jarchau,
Cloning, expression, and in situ localization of rat intestinal cGMP-dependent protein kinase II.
1994,
Pubmed
Kojima,
Urinary excretion of atrial natriuretic peptide during saline infusion and supraventricular tachycardia.
1989,
Pubmed
Koller,
Selective activation of the B natriuretic peptide receptor by C-type natriuretic peptide (CNP).
1991,
Pubmed
Light,
Amiloride-sensitive cation channel in apical membrane of inner medullary collecting duct.
1988,
Pubmed
Light,
Atrial natriuretic peptide inhibits a cation channel in renal inner medullary collecting duct cells.
1989,
Pubmed
Marunaka,
Regulation of an amiloride-sensitive Na+-permeable channel by a beta2-adrenergic agonist, cytosolic Ca2+ and Cl- in fetal rat alveolar epithelium.
1999,
Pubmed
Meier,
Functional analysis of natriuretic peptide receptors in the bladder of the toad, Bufo marinus.
2002,
Pubmed
Murthy,
G(i-1)/G(i-2)-dependent signaling by single-transmembrane natriuretic peptide clearance receptor.
2000,
Pubmed
Nie,
Regulation of epithelial sodium channels by cGMP/PKGII.
2009,
Pubmed
,
Xenbase
Nie,
8-pCPT-cGMP stimulates alphabetagamma-ENaC activity in oocytes as an external ligand requiring specific nucleotide moieties.
2010,
Pubmed
,
Xenbase
Poschet,
Pharmacological modulation of cGMP levels by phosphodiesterase 5 inhibitors as a therapeutic strategy for treatment of respiratory pathology in cystic fibrosis.
2007,
Pubmed
Ritter,
Polarized distribution of renal natriuretic peptide receptors in normal physiology and ischemia.
1995,
Pubmed
Spiegel,
Epithelial polarity following ischemia: a requirement for normal cell function.
1989,
Pubmed
Suga,
Receptor selectivity of natriuretic peptide family, atrial natriuretic peptide, brain natriuretic peptide, and C-type natriuretic peptide.
1992,
Pubmed
Vesely,
Five cardiac hormones decrease the number of human small-cell lung cancer cells.
2005,
Pubmed
Vesely,
Elimination of up to 80% of human pancreatic adenocarcinomas in athymic mice by cardiac hormones.
2007,
Pubmed
Yamada,
Frog atrial natriuretic peptide and cGMP activate amiloride-sensitive Na(+) channels in urinary bladder cells of Japanese tree frog, Hyla japonica.
2007,
Pubmed
Yamada,
Frog ANP increases the amiloride-sensitive Na(+) channel activity in urinary bladder cells of Japanese tree frog, Hyla japonica.
2007,
Pubmed
Yamamoto,
Expression of mRNA for natriuretic peptide receptor subtypes in bovine kidney.
1994,
Pubmed
Yu,
Aldosterone-induced increases in superoxide production counters nitric oxide inhibition of epithelial Na channel activity in A6 distal nephron cells.
2007,
Pubmed
,
Xenbase
Zeidel,
cGMP mediates effects of atrial peptides on medullary collecting duct cells.
1987,
Pubmed
Zeidel,
Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells.
1988,
Pubmed
Zhao,
ANP-mediated inhibition of distal nephron fractional sodium reabsorption in wild-type and mice overexpressing natriuretic peptide receptor.
2010,
Pubmed
