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Int J Dev Biol
2012 Jan 01;5610-12:975-86. doi: 10.1387/ijdb.120150jg.
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Amphibian interorder nuclear transfer embryos reveal conserved embryonic gene transcription, but deficient DNA replication or chromosome segregation.
Narbonne P
,
Gurdon JB
.
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Early interspecies nuclear transfer (iNT) experiments suggested that a foreign nucleus may become permanently damaged after a few rounds of cell division in the cytoplasm of another species. That is, in some distant species combinations, nucleocytoplasmic hybrid (cybrid) blastula nuclei can no longer support development, even if they are back-transferred into their own kind of eggcytoplasm. We monitored foreign DNA amplification and RNA production by quantitative PCR (qPCR) and RT-qPCR in interorder amphibian hybrids and cybrids formed by the transfer of newt (Pleurodeles waltl) embryonic nuclei into intact and enucleated frog (Xenopus laevis) eggs. We found a dramatic reduction in the expansion of foreign DNA and cell numbers in developing cybrid embryos that correlated with reduced gene transcription. Interestingly, expansion in cell numbers was rescued by the recipient species (Xenopus) maternal genome in iNT hybrids, but it did not improve P. waltl DNA expansion or gene transcription. Also, foreign gene transcripts, normalized to DNA copy numbers, were mostly normal in both iNT hybrids and cybrids. Thus, incomplete foreign DNA replication and/or chromosome segregation during cell division may be the major form of nuclear damage occurring as a result of nuclear replication in a foreign cytoplasmic environment. It also shows that the mechanisms of embryonic gene transcription are highly conserved across amphibians and may not be a major cause of cybrid lethality.
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Bailey,
Phylogenetic reconstruction of vertebrate Hox cluster duplications.
1997, Pubmed
Bailey,
Phylogenetic reconstruction of vertebrate Hox cluster duplications.
1997,
Pubmed
Bascove,
Molecular characterization of Pleurodeles waltl activation-induced cytidine deaminase.
2010,
Pubmed
,
Xenbase
Beaujean,
The effect of interspecific oocytes on demethylation of sperm DNA.
2004,
Pubmed
Benton,
Paleontological evidence to date the tree of life.
2007,
Pubmed
Beyhan,
Interspecies nuclear transfer: implications for embryonic stem cell biology.
2007,
Pubmed
Birchler,
A tale of two centromeres--diversity of structure but conservation of function in plants and animals.
2009,
Pubmed
Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed
Briggs,
Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.
1952,
Pubmed
Cannon,
Xenomitochondrial mice: investigation into mitochondrial compensatory mechanisms.
2011,
Pubmed
Chung,
Reprogramming of human somatic cells using human and animal oocytes.
2009,
Pubmed
Dawid,
Deoxyribonucleic acid in amphibian eggs.
1965,
Pubmed
Dimitrova,
Regulation of mammalian replication origin usage in Xenopus egg extract.
1998,
Pubmed
,
Xenbase
ELSDALE,
A description of the technique for nuclear transplantation in Xenopus laevis.
1960,
Pubmed
,
Xenbase
Egli,
Recipient cell nuclear factors are required for reprogramming by nuclear transfer.
2010,
Pubmed
Evans,
A mitochondrial DNA phylogeny of African clawed frogs: phylogeography and implications for polyploid evolution.
2004,
Pubmed
,
Xenbase
Fujimoto,
Developmental potential of embryonic cells in a nucleocytoplasmic hybrid formed using a goldfish haploid nucleus and loach egg cytoplasm.
2010,
Pubmed
Fujiwara,
Uniparental chromosome elimination in the early embryogenesis of the inviable salmonid hybrids between masu salmon female and rainbow trout male.
1997,
Pubmed
GURDON,
The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis.
1960,
Pubmed
,
Xenbase
GURDON,
Tetraploid frogs.
1959,
Pubmed
,
Xenbase
GURDON,
The transplantation of nuclei between two species of Xenopus.
1962,
Pubmed
,
Xenbase
Gallien,
Nucleocytoplasmic interactions during ontogenesis in individuals obtained by intra- and interspecific nuclear transplantation in the genus Pleurodeles (urodele amphibian).
1973,
Pubmed
Ganier,
Synergic reprogramming of mammalian cells by combined exposure to mitotic Xenopus egg extracts and transcription factors.
2011,
Pubmed
,
Xenbase
Gómez,
Trichostatin A modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viability.
2011,
Pubmed
HAMILTON,
An experimental analysis of the development of the haploid syndrome in embryos of Xenopus laevis.
1963,
Pubmed
,
Xenbase
Halley-Stott,
Mammalian nuclear transplantation to Germinal Vesicle stage Xenopus oocytes - a method for quantitative transcriptional reprogramming.
2010,
Pubmed
,
Xenbase
Henikoff,
The centromere paradox: stable inheritance with rapidly evolving DNA.
2001,
Pubmed
Hennen,
Back-transfer of late gastrula nuclei of nucleocytoplasmic hybrids.
1974,
Pubmed
Hurley,
A new time-scale for ray-finned fish evolution.
2007,
Pubmed
Hyrien,
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
1993,
Pubmed
,
Xenbase
Janvier,
Palaeontology: modern look for ancient lamprey.
2006,
Pubmed
Kenyon,
Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids.
1997,
Pubmed
Kim,
An interclass nuclear transfer between fowl and mammal: in vitro development of chicken-to-cattle interclass embryos and the detection of chicken genetic complements.
2004,
Pubmed
Lee,
Enhanced histone acetylation in somatic cells induced by a histone deacetylase inhibitor improved inter-generic cloned leopard cat blastocysts.
2010,
Pubmed
Levy,
Nuclear size is regulated by importin α and Ntf2 in Xenopus.
2010,
Pubmed
,
Xenbase
Liu,
Blastocysts produced by nuclear transfer between chicken blastodermal cells and rabbit oocytes.
2004,
Pubmed
Loi,
Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid.
2011,
Pubmed
Loughlin,
Katanin contributes to interspecies spindle length scaling in Xenopus.
2011,
Pubmed
,
Xenbase
Luo,
A Jurassic eutherian mammal and divergence of marsupials and placentals.
2011,
Pubmed
MOORE,
Transplantation of nuclei between Rana pipiens and Rana sylvatica.
1958,
Pubmed
McKenzie,
Functional respiratory chain analyses in murid xenomitochondrial cybrids expose coevolutionary constraints of cytochrome b and nuclear subunits of complex III.
2003,
Pubmed
Mizutani,
Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones.
2012,
Pubmed
Narbonne,
Deficient induction response in a Xenopus nucleocytoplasmic hybrid.
2011,
Pubmed
,
Xenbase
Narbonne,
Reprogramming and development in nuclear transfer embryos and in interspecific systems.
2012,
Pubmed
Narbonne,
On the cellular and developmental lethality of a Xenopus nucleocytoplasmic hybrid.
2012,
Pubmed
,
Xenbase
Noggle,
Human oocytes reprogram somatic cells to a pluripotent state.
2011,
Pubmed
Nomiyama,
A family tree of vertebrate chemokine receptors for a unified nomenclature.
2011,
Pubmed
PICHERAL,
[Capacities of the nuclei of embryonic endodermal cells to organize a viable germ in the urodele, Pleurodeles waltlii Michah].
1962,
Pubmed
Rajarajan,
Reprogramming of mouse, rat, pig, and human fibroblasts into iPS cells.
2012,
Pubmed
Roelants,
Global patterns of diversification in the history of modern amphibians.
2007,
Pubmed
Sakai,
Chromosome elimination in the interspecific hybrid medaka between Oryzias latipes and O. hubbsi.
2007,
Pubmed
Sha,
Fates of donor and recipient mitochondrial DNA during generation of interspecies SCNT-derived human ES-like cells.
2009,
Pubmed
Shi,
The chronological development of the urodele amphibian Pleurodeles waltl (Michah).
1995,
Pubmed
,
Xenbase
Shi,
Trichostatin A (TSA) improves the development of rabbit-rabbit intraspecies cloned embryos, but not rabbit-human interspecies cloned embryos.
2008,
Pubmed
Sotomaru,
Preimplantation development of somatic cell cloned embryos in the common marmoset (Callithrix jacchus).
2009,
Pubmed
Tecirlioglu,
Interspecies somatic cell nuclear transfer and preliminary data for horse-cow/mouse iSCNT.
2006,
Pubmed
Wang,
Bovine ooplasm partially remodels primate somatic nuclei following somatic cell nuclear transfer.
2009,
Pubmed
Wang,
Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos.
2011,
Pubmed
Wheatley,
Inhibition of chromosomal separation provides insights into cleavage furrow stimulation in cultured epithelial cells.
1998,
Pubmed
Woodland,
RNA synthesis in an amphibian nuclear-transplant hybrid.
1969,
Pubmed
Yan,
Developmental incompatibility between cell nucleus and cytoplasm as revealed by nuclear transplantation experiments in teleost of different families and orders.
1990,
Pubmed
Yan,
Further investigation on nuclear transplantation in different orders of teleost: the combination of the nucleus of Tilapia (Oreochromis nilotica) and the cytoplasm of Loach (Paramisgurnus dabryanus).
1991,
Pubmed
Yang,
High-efficiency somatic reprogramming induced by intact MII oocytes.
2010,
Pubmed
