Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2003 Oct 01;2319:6769-79. doi: 10.1128/MCB.23.19.6769-6779.2003.
Show Gene links
Show Anatomy links
CpG methylation of DNA restricts prereplication complex assembly in Xenopus egg extracts.
Harvey KJ
,
Newport J
.
???displayArticle.abstract???
In a Xenopus egg replication system, the origin recognition complex (ORC) does not bind to CpG methylated DNA and DNA replication is inhibited. Insertion of low density CpG DNA of at least 1.2 kb into methylated plasmids rescues both replication and ORC binding. Using this pseudo-origin, we find that ORC binding is restricted to low-CpG-density DNA; however, MCM is loaded onto both weakly and highly methylated DNA and occupies at least approximately 2 kb of DNA. Replication initiates coincident with MCM, and even the most distally bound MCM is associated with sites of replication initiation. These results suggest that in metazoans MCM is loaded onto and initiates replication over a large region distant from ORC.
Abdurashidova,
Start sites of bidirectional DNA synthesis at the human lamin B2 origin.
2000, Pubmed
Abdurashidova,
Start sites of bidirectional DNA synthesis at the human lamin B2 origin.
2000,
Pubmed
Aladjem,
Genetic dissection of a mammalian replicator in the human beta-globin locus.
1998,
Pubmed
Alexandrow,
A potential role for mini-chromosome maintenance (MCM) proteins in initiation at the dihydrofolate reductase replication origin.
2002,
Pubmed
Antequera,
CpG islands as genomic footprints of promoters that are associated with replication origins.
1999,
Pubmed
Araujo,
Concurrent replication and methylation at mammalian origins of replication.
1998,
Pubmed
Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed
Bell,
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
1992,
Pubmed
Bielinsky,
Discrete start sites for DNA synthesis in the yeast ARS1 origin.
1998,
Pubmed
Bielinsky,
Origin recognition complex binding to a metazoan replication origin.
2001,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Bosco,
DNA replication control through interaction of E2F-RB and the origin recognition complex.
2001,
Pubmed
Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed
Campanero,
CpG methylation as a mechanism for the regulation of E2F activity.
2000,
Pubmed
Carpenter,
Role for a Xenopus Orc2-related protein in controlling DNA replication.
1996,
Pubmed
,
Xenbase
Chesnokov,
Functional analysis of mutant and wild-type Drosophila origin recognition complex.
2001,
Pubmed
Chuang,
Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein.
2002,
Pubmed
Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase
Costanzo,
Reconstitution of an ATM-dependent checkpoint that inhibits chromosomal DNA replication following DNA damage.
2000,
Pubmed
,
Xenbase
Dasso,
RCC1, a regulator of mitosis, is essential for DNA replication.
1992,
Pubmed
,
Xenbase
Delgado,
Initiation of DNA replication at CpG islands in mammalian chromosomes.
1998,
Pubmed
Diffley,
Two steps in the assembly of complexes at yeast replication origins in vivo.
1994,
Pubmed
,
Xenbase
Dijkwel,
Initiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficiencies.
2002,
Pubmed
Dimitrova,
Temporally coordinated assembly and disassembly of replication factories in the absence of DNA synthesis.
2000,
Pubmed
Dobbs,
Modular sequence elements associated with origin regions in eukaryotic chromosomal DNA.
1994,
Pubmed
Donovan,
Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast.
1997,
Pubmed
,
Xenbase
Edwards,
MCM2-7 complexes bind chromatin in a distributed pattern surrounding the origin recognition complex in Xenopus egg extracts.
2002,
Pubmed
,
Xenbase
Gerbi,
DNA replication and chromatin.
2002,
Pubmed
Gilbert,
Making sense of eukaryotic DNA replication origins.
2001,
Pubmed
Gowher,
DNA of Drosophila melanogaster contains 5-methylcytosine.
2000,
Pubmed
Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Hua,
Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2.
1998,
Pubmed
,
Xenbase
Hyrien,
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
1993,
Pubmed
,
Xenbase
Hyrien,
Transition in specification of embryonic metazoan DNA replication origins.
1995,
Pubmed
,
Xenbase
Jones,
Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription.
1998,
Pubmed
,
Xenbase
Krysan,
Isolation of human sequences that replicate autonomously in human cells.
1989,
Pubmed
Lawlis,
Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Lucas,
Mechanisms ensuring rapid and complete DNA replication despite random initiation in Xenopus early embryos.
2000,
Pubmed
,
Xenbase
Lunyak,
Developmental changes in the Sciara II/9A initiation zone for DNA replication.
2002,
Pubmed
Lyko,
DNA methylation in Drosophila melanogaster.
2000,
Pubmed
Mahbubani,
Cell cycle regulation of the replication licensing system: involvement of a Cdk-dependent inhibitor.
1997,
Pubmed
,
Xenbase
Mahbubani,
DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Maiorano,
XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Malott,
Activity of the c-myc replicator at an ectopic chromosomal location.
1999,
Pubmed
McWhinney,
Autonomous replication of a DNA fragment containing the chromosomal replication origin of the human c-myc gene.
1990,
Pubmed
Nan,
MeCP2 is a transcriptional repressor with abundant binding sites in genomic chromatin.
1997,
Pubmed
Nightingale,
Methylation at CpG sequences does not influence histone H1 binding to a nucleosome including a Xenopus borealis 5 S rRNA gene.
1995,
Pubmed
,
Xenbase
Rein,
DNA methylation at mammalian replication origins.
1999,
Pubmed
Rippe,
Making contacts on a nucleic acid polymer.
2001,
Pubmed
Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase
Rowles,
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
1996,
Pubmed
,
Xenbase
Rowles,
Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins.
1999,
Pubmed
,
Xenbase
Royzman,
ORC localization in Drosophila follicle cells and the effects of mutations in dE2F and dDP.
1999,
Pubmed
Simon,
Asynchronous replication of imprinted genes is established in the gametes and maintained during development.
1999,
Pubmed
Smythe,
Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts.
1991,
Pubmed
,
Xenbase
Sterner,
Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.
1998,
Pubmed
,
Xenbase
Veenstra,
Constitutive genomic methylation during embryonic development of Xenopus.
2001,
Pubmed
,
Xenbase
Walter,
Regulation of replicon size in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Walter,
Regulated chromosomal DNA replication in the absence of a nucleus.
1998,
Pubmed
,
Xenbase
Walter,
Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha.
2000,
Pubmed
,
Xenbase
Waltz,
DNA replication initiates non-randomly at multiple sites near the c-myc gene in HeLa cells.
1996,
Pubmed
Wolffe,
Epigenetics: regulation through repression.
1999,
Pubmed
Wyrick,
Genome-wide distribution of ORC and MCM proteins in S. cerevisiae: high-resolution mapping of replication origins.
2001,
Pubmed
