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Proc Natl Acad Sci U S A June 11, 2013; 110 (24): 9903-8.
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Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12).

Chowanadisai W , Graham DM , Keen CL , Rucker RB , Messerli MA .

Zn(2+) is required for many aspects of neuronal structure and function. However, the regulation of Zn(2+) in the nervous system remains poorly understood. Systematic analysis of tissue-profiling microarray data showed that the zinc transporter ZIP12 (slc39a12) is highly expressed in the human brain. In the work reported here, we confirmed that ZIP12 is a Zn(2+) uptake transporter with a conserved pattern of high expression in the mouse and Xenopus nervous system. Mouse neurons and Neuro-2a cells produce fewer and shorter neurites after ZIP12 knockdown without affecting cell viability. Zn(2+) chelation or loading in cells to alter Zn(2+) availability respectively mimicked or reduced the effects of ZIP12 knockdown on neurite outgrowth. ZIP12 knockdown reduces cAMP response element-binding protein activation and phosphorylation at serine 133, which is a critical pathway for neuronal differentiation. Constitutive cAMP response element-binding protein activation restores impairments in neurite outgrowth caused by Zn(2+) chelation or ZIP12 knockdown. ZIP12 knockdown also reduces tubulin polymerization and increases sensitivity to nocodazole following neurite outgrowth. We find that ZIP12 is expressed during neurulation and early nervous system development in Xenopus tropicalis, where ZIP12 antisense morpholino knockdown impairs neural tube closure and arrests development during neurulation with concomitant reduction in tubulin polymerization in the neural plate. This study identifies a Zn(2+) transporter that is specifically required for nervous system development and provides tangible links between Zn(2+), neurulation, and neuronal differentiation.

PubMed ID: 23716681
PMC ID: PMC3683776
Article link: Proc Natl Acad Sci U S A
Grant support: [+]

Species referenced: Xenopus tropicalis
Genes referenced: actl6a creb1 ctnnb1 slc39a12
GO keywords: neural tube closure [+]
Morpholinos: slc39a12 MO1 slc39a12 MO2

Phenotypes: Xtr Wt + slc39a12 MO (Fig. 5G) [+]

Article Images: [+] show captions
References [+] :
Ala, Prediction of human disease genes by human-mouse conserved coexpression analysis. 2008, Pubmed