Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Planta
1988 Mar 01;1733:289-97. doi: 10.1007/BF00401015.
Show Gene links
Show Anatomy links
Glyoxysomal citrate synthase from watermelon cotyledons: immunocytochemical localization and heterologous translation in Xenopus oocytes.
Sautter C
,
Keller G
,
Hock B
.
???displayArticle.abstract???
Glyoxysomal citrate synthase (gCS) was purified from crude extracts of watermelon (Citrullus vulgaris Schrad.) cotyledons, yielding a homogenous protein with a subunit MW of 48 kDa. The enzyme was selectively inhibited by 5,5'-dithiobis-(2-nitrobenzoic acid), allowing quantification in the presence of the mitochondrial isoenzyme (mCS). Differences were also observed with respect to inhibition by ATP (k i=2.6 mmol · l(-1) for gCS, k i=0.33 mmol · l(-1) for mCS). The antibodies prepared against gCS did not cross-react with mCS. The immunocytochemical localization of gCS by the indirect protein A-gold procedure was restricted to the glyoxysomal membrane or the peripheral matrix of glyoxysomes. Other compartments, e.g. the endoplasmic reticulum, were not labeled. Xenopus oocytes were used for the translation of watermelon polyadenylated RNA (poly(A)(+)RNA). A translation product with a MW of 51 kDa was immunoprecipitated by the anti-gCS antibodies. It was absent in controls without poly(A)(+)RNA or with preimmune serum. A similar translation product was also immunoprecipitated after cell-free synthesis of watermelon poly(A)(+)RNA in a reticulocyte system, in contrast to the in-vivo labeled gCS (48 kDa). It was concluded that gCS is synthesized as a higher-molecular-weight precursor.
Alam,
In vitro translation of mRNA for yeast citrate synthase.
1982, Pubmed
Alam,
In vitro translation of mRNA for yeast citrate synthase.
1982,
Pubmed
Axelrod,
Differential response of mitochondrial and glyoxysomal citrate synthase to ATP.
1972,
Pubmed
Barbareschi,
Citrate synthetase in mitochondria and glyoxysomes of maize scutellum.
1974,
Pubmed
Bassüner,
Secretion of plant storage globulin polypeptides by Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase
Beeckmans,
Some structural and regulatory aspects of citrate synthase.
1984,
Pubmed
Bendayan,
Immunocytochemical localization of fatty acid metabolizing heat-stable and heat-labile enoyl-coenzyme A (CoA) hydratases in liver and renal cortex.
1983,
Pubmed
Blobel,
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
1975,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Breidenbach,
Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm.
1967,
Pubmed
Fujiki,
Synthesis of a major integral membrane polypeptide of rat liver peroxisomes on free polysomes.
1984,
Pubmed
Gietl,
Import of glyoxysomal malate dehydrogenase precursor into glyoxysomes: A heterologous in-vitro system.
1986,
Pubmed
Gietl,
Organelle-bound malate dehydrogenase isoenzymes are synthesized as higher molecular weight precursors.
1982,
Pubmed
Harmey,
Biosynthesis of mitochondrial citrate synthase in Neurospora crassa.
1979,
Pubmed
Hock,
[Inhibition of isocitrate lyase in watermelon seedlings by white light].
1969,
Pubmed
Huang,
Localization of enzymes within microbodies.
1973,
Pubmed
Huang,
Immunological and biochemical studies on isozymes of malate dehydrogenase and citrate synthetase in castor bean glyoxysomes.
1974,
Pubmed
Hurkman,
Subcellular compartmentalization of maize storage proteins in Xenopus oocytes injected with zein messenger RNAs.
1981,
Pubmed
,
Xenbase
Kagawa,
Organelle-specific isozymes of citrate synthase in the endosperm of developing ricinus seedlings.
1981,
Pubmed
Köller,
Glyoxylate cycle enzymes of the glyoxysomal membrane from cucumber cotyledons.
1977,
Pubmed
Köster,
In vitro synthesis of peroxisomal membrane polypeptides.
1986,
Pubmed
Sautter,
Separation of plant cell organelles by zonal centrifugation in reorienting density gradients.
1981,
Pubmed
Titus,
Investigation of the glyoxysome-peroxisome transition in germinating cucumber cotyledons using double-label immunoelectron microscopy.
1985,
Pubmed
Van den Bosch,
Immunogold localization of nodule-specific uricase in developing soybean root nodules.
1986,
Pubmed
Vitale,
Regulation of processing of a plant glycoprotein in the Golgi complex: A comparative study usingXenopus oocytes.
1986,
Pubmed
Walk,
Glyoxysomal malate dehydrogenase of watermelon cotyledons: De novo synthesis on cytoplasmic ribosomes.
1977,
Pubmed
Walk,
Cell-free synthesis of glyoxysomal malate dehydrogenase.
1978,
Pubmed
Wanner,
Ontogeny of microbodies (glyoxysomes) in cotyledons of dark-grown watermelon (Citrullus vulgaris Schrad.) seedlings : Ultrastructural evidence.
1982,
Pubmed
Yokota,
Immunocytochemical localization of two peroxisomal enzymes of lipid beta-oxidation in specific granules of rat eosinophils.
1983,
Pubmed