Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Int J Mol Sci February 11, 2014; 15 (2): 2431-53.
Show Gene links Show Anatomy links

PhosphoTyrosyl phosphatase activator of Plasmodium falciparum: identification of its residues involved in binding to and activation of PP2A.

Vandomme A , Fréville A , Cailliau K , Kalamou H , Bodart JF , Khalife J , Pierrot C .

In Plasmodium falciparum (Pf), the causative agent of the deadliest form of malaria, a tight regulation of phosphatase activity is crucial for the development of the parasite. In this study, we have identified and characterized PfPTPA homologous to PhosphoTyrosyl Phosphatase Activator, an activator of protein phosphatase 2A which is a major phosphatase involved in many biological processes in eukaryotic cells. The PfPTPA sequence analysis revealed that five out of six amino acids involved in interaction with PP2A in human are conserved in P. falciparum. Localization studies showed that PfPTPA and PfPP2A are present in the same compartment of blood stage parasites, suggesting a possible interaction of both proteins. In vitro binding and functional studies revealed that PfPTPA binds to and activates PP2A. Mutation studies showed that three residues (V(283), G(292) and M(296)) of PfPTPA are indispensable for the interaction and that the G(292) residue is essential for its activity. In P. falciparum, genetic studies suggested the essentiality of PfPTPA for the completion of intraerythrocytic parasite lifecycle. Using Xenopus oocytes, we showed that PfPTPA blocked the G2/M transition. Taken together, our data suggest that PfPTPA could play a role in the regulation of the P. falciparum cell cycle through its PfPP2A regulatory activity.

PubMed ID: 24521882
PMC ID: PMC3958860
Article link: Int J Mol Sci

Species referenced: Xenopus
Genes referenced: endoul myc npy4r ptpa set

Article Images: [+] show captions
References [+] :
Aurrecoechea, PlasmoDB: a functional genomic database for malaria parasites. 2008, Pubmed