Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Formation of the vertebrate brain ventricles requires both production of cerebrospinal fluid (CSF), and its retention in the ventricles. The Na,K-ATPase is required for brain ventricle development, and we show here that this protein complex impacts three associated processes. The first requires both the alpha subunit (Atp1a1) and the regulatory subunit, Fxyd1, and leads to formation of a cohesive neuroepithelium, with continuous apical junctions. The second process leads to modulation of neuroepithelial permeability, and requires Atp1a1, which increases permeability with partial loss of function and decreases it with overexpression. In contrast, fxyd1 overexpression does not alter neuroepithelial permeability, suggesting that its activity is limited to neuroepithelium formation. RhoA regulates both neuroepithelium formation and permeability, downstream of the Na,K-ATPase. A third process, likely to be CSF production, is RhoA-independent, requiring Atp1a1, but not Fxyd1. Consistent with a role for Na,K-ATPase pump function, the inhibitor ouabain prevents neuroepithelium formation, while intracellular Na(+) increases after Atp1a1 and Fxyd1 loss of function. These data include the first reported role for Fxyd1 in the developing brain, and indicate that the Na,K-ATPase regulates three aspects of brain ventricle development essential for normal function: formation of a cohesive neuroepithelium, restriction of neuroepithelial permeability, and production of CSF.
Amano,
Regulation and functions of Rho-associated kinase.
2000, Pubmed
Amano,
Regulation and functions of Rho-associated kinase.
2000,
Pubmed
Bagnat,
Genetic control of single lumen formation in the zebrafish gut.
2007,
Pubmed
Bibert,
Phosphorylation of phospholemman (FXYD1) by protein kinases A and C modulates distinct Na,K-ATPase isozymes.
2008,
Pubmed
,
Xenbase
Bossuyt,
Phospholemman phosphorylation alters its fluorescence resonance energy transfer with the Na/K-ATPase pump.
2006,
Pubmed
Brown,
Molecular mechanisms of cerebrospinal fluid production.
2004,
Pubmed
Canfield,
Na,K-ATPase alpha and beta subunit genes exhibit unique expression patterns during zebrafish embryogenesis.
2002,
Pubmed
Cibrián-Uhalte,
Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance.
2007,
Pubmed
Ciruna,
Planar cell polarity signalling couples cell division and morphogenesis during neurulation.
2006,
Pubmed
Crambert,
Phospholemman (FXYD1) associates with Na,K-ATPase and regulates its transport properties.
2002,
Pubmed
,
Xenbase
Dahl,
Participation of Na,K-ATPase in FGF-2 secretion: rescue of ouabain-inhibitable FGF-2 secretion by ouabain-resistant Na,K-ATPase alpha subunits.
2000,
Pubmed
Desmond,
Internal luminal pressure during early chick embryonic brain growth: descriptive and empirical observations.
2005,
Pubmed
Draper,
Inhibition of zebrafish fgf8 pre-mRNA splicing with morpholino oligos: a quantifiable method for gene knockdown.
2001,
Pubmed
,
Xenbase
Ellertsdottir,
A mutation in the zebrafish Na,K-ATPase subunit atp1a1a.1 provides genetic evidence that the sodium potassium pump contributes to left-right asymmetry downstream or in parallel to nodal flow.
2006,
Pubmed
Feschenko,
Phospholemman, a single-span membrane protein, is an accessory protein of Na,K-ATPase in cerebellum and choroid plexus.
2003,
Pubmed
Gato,
Embryonic cerebrospinal fluid regulates neuroepithelial survival, proliferation, and neurogenesis in chick embryos.
2005,
Pubmed
Geering,
The functional role of beta subunits in oligomeric P-type ATPases.
2001,
Pubmed
Geering,
FXYD proteins: new regulators of Na-K-ATPase.
2006,
Pubmed
Graeden,
Live imaging of the zebrafish embryonic brain by confocal microscopy.
2009,
Pubmed
Gutzman,
Zebrafish brain ventricle injection.
2009,
Pubmed
Gutzman,
Epithelial relaxation mediated by the myosin phosphatase regulator Mypt1 is required for brain ventricle lumen expansion and hindbrain morphogenesis.
2010,
Pubmed
,
Xenbase
Hong,
N-cadherin is required for the polarized cell behaviors that drive neurulation in the zebrafish.
2006,
Pubmed
Jia,
Hypertrophy, increased ejection fraction, and reduced Na-K-ATPase activity in phospholemman-deficient mice.
2005,
Pubmed
Jiang,
Mutations affecting neurogenesis and brain morphology in the zebrafish, Danio rerio.
1996,
Pubmed
Kimmel,
Stages of embryonic development of the zebrafish.
1995,
Pubmed
,
Xenbase
Knust,
Composition and formation of intercellular junctions in epithelial cells.
2002,
Pubmed
Krupinski,
Unexpected roles of the Na-K-ATPase and other ion transporters in cell junctions and tubulogenesis.
2009,
Pubmed
Lansbery,
Cytoplasmic targeting signals mediate delivery of phospholemman to the plasma membrane.
2006,
Pubmed
Linask,
Inhibitory effects of ouabain on early heart development and cardiomyogenesis in the chick embryo.
1995,
Pubmed
Lowery,
Initial formation of zebrafish brain ventricles occurs independently of circulation and requires the nagie oko and snakehead/atp1a1a.1 gene products.
2005,
Pubmed
Lowery,
Characterization and classification of zebrafish brain morphology mutants.
2009,
Pubmed
Lowery,
Totally tubular: the mystery behind function and origin of the brain ventricular system.
2009,
Pubmed
,
Xenbase
Mishra,
FXYD proteins stabilize Na,K-ATPase: amplification of specific phosphatidylserine-protein interactions.
2011,
Pubmed
Morth,
Crystal structure of the sodium-potassium pump.
2007,
Pubmed
Nasevicius,
Effective targeted gene 'knockdown' in zebrafish.
2000,
Pubmed
Oshio,
Aquaporin-1 deletion reduces osmotic water permeability and cerebrospinal fluid production.
2003,
Pubmed
Paul,
A pump-independent function of the Na,K-ATPase is required for epithelial junction function and tracheal tube-size control.
2007,
Pubmed
Pavlović,
The intracellular region of FXYD1 is sufficient to regulate cardiac Na/K ATPase.
2007,
Pubmed
Pollay,
Choroid plexus Na+/K+-activated adenosine triphosphatase and cerebrospinal fluid formation.
1985,
Pubmed
Rajasekaran,
Na,K-ATPase activity is required for formation of tight junctions, desmosomes, and induction of polarity in epithelial cells.
2001,
Pubmed
Rajasekaran,
Na-K-ATPase regulates tight junction permeability through occludin phosphorylation in pancreatic epithelial cells.
2007,
Pubmed
Sagerström,
Anteroposterior patterning in the zebrafish, Danio rerio: an explant assay reveals inductive and suppressive cell interactions.
1996,
Pubmed
Shinoda,
Crystal structure of the sodium-potassium pump at 2.4 A resolution.
2009,
Pubmed
Shu,
Na,K-ATPase is essential for embryonic heart development in the zebrafish.
2003,
Pubmed
Speake,
Mechanisms of CSF secretion by the choroid plexus.
2001,
Pubmed
Sprague,
The Zebrafish Information Network (ZFIN): a resource for genetic, genomic and developmental research.
2001,
Pubmed
Sweadner,
The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression.
2000,
Pubmed
Szászi,
Depolarization induces Rho-Rho kinase-mediated myosin light chain phosphorylation in kidney tubular cells.
2005,
Pubmed
Terry,
Rho signaling and tight junction functions.
2010,
Pubmed
Ulitsky,
Conserved function of lincRNAs in vertebrate embryonic development despite rapid sequence evolution.
2011,
Pubmed
Waheed,
Extracellular signal-regulated kinase and GEF-H1 mediate depolarization-induced Rho activation and paracellular permeability increase.
2010,
Pubmed
Wilson,
Apical plasma membrane mispolarization of NaK-ATPase in polycystic kidney disease epithelia is associated with aberrant expression of the beta2 isoform.
2000,
Pubmed
Yamamoto,
Phosphorylation of claudin-5 and occludin by rho kinase in brain endothelial cells.
2008,
Pubmed
Yuan,
The small heart mutation reveals novel roles of Na+/K+-ATPase in maintaining ventricular cardiomyocyte morphology and viability in zebrafish.
2004,
Pubmed
Zhang,
Phospholemman modulates Na+/Ca2+ exchange in adult rat cardiac myocytes.
2003,
Pubmed
Zhang,
Establishment of a neuroepithelial barrier by Claudin5a is essential for zebrafish brain ventricular lumen expansion.
2010,
Pubmed
