Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-49432
Mol Brain September 16, 2014; 7 67.
Show Gene links Show Anatomy links

5-Mehtyltetrahydrofolate rescues alcohol-induced neural crest cell migration abnormalities.

Shi Y , Li J , Chen C , Gong M , Chen Y , Liu Y , Chen J , Li T , Song W .


Abstract
BACKGROUND: Alcohol is detrimental to early development. Fetal alcohol spectrum disorders (FASD) due to maternal alcohol abuse results in a series of developmental abnormalities including cranial facial dysmorphology, ocular anomalies, congenital heart defects, microcephaly and intellectual disabilities. Previous studies have been shown that ethanol exposure causes neural crest (NC) apoptosis and perturbation of neural crest migration. However, the underlying mechanism remains elusive. In this report we investigated the fetal effect of alcohol on the process of neural crest development in the Xenopus leavis. RESULTS: Pre-gastrulation exposure of 2-4% alcohol induces apoptosis in Xenopus embryo whereas 1% alcohol specifically impairs neural crest migration without observing discernible apoptosis. Additionally, 1% alcohol treatment considerably increased the phenotype of small head (43.4% ± 4.4%, total embryo n = 234), and 1.5% and 2.0% dramatically augment the deformation to 81.2% ± 6.5% (n = 205) and 91.6% ± 3.0% (n = 235), respectively (P < 0.05). Significant accumulation of Homocysteine was caused by alcohol treatment in embryos and 5-mehtyltetrahydrofolate restores neural crest migration and alleviates homocysteine accumulation, resulting in inhibition of the alcohol-induced neurocristopathies. CONCLUSIONS: Our study demonstrates that prenatal alcohol exposure causes neural crest cell migration abnormality and 5-mehtyltetrahydrofolate could be beneficial for treating FASD.

PubMed ID: 25223405
PMC ID: PMC4172781
Article link: Mol Brain
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actb actl6a msx1 pax3 snai2 twist1 zic1
Antibodies: Casp3 Ab5 H3f3a Ab12

Disease Ontology terms: fetal alcohol spectrum disorder
Phenotypes: Xla Wt + EtOH (Fig.1.B,D) [+]

Article Images: [+] show captions
References [+] :
Aybar, Snail precedes slug in the genetic cascade required for the specification and migration of the Xenopus neural crest. 2002, Pubmed, Xenbase