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Nat Commun March 31, 2015; 6 6666.
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ERK7 regulates ciliogenesis by phosphorylating the actin regulator CapZIP in cooperation with Dishevelled.

Miyatake K , Kusakabe M , Takahashi C , Nishida E .

Cilia are essential for embryogenesis and maintenance of homeostasis, but little is known about the signalling pathways that regulate ciliogenesis. Here, we identify ERK7, an atypical mitogen-activated protein kinase, as a key regulator of ciliogenesis. ERK7 is strongly expressed in ciliated tissues of Xenopus embryos. ERK7 knockdown markedly diminishes both the number and the length of cilia in multiciliated cells, and it inhibits the apical migration of basal bodies. Moreover, ERK7 knockdown results in a loss of the apical actin meshwork, which is required for the proper migration of basal bodies. We find that the actin regulator CapZIP, which has been shown to regulate ciliogenesis in a phosphorylation-dependent manner, is an ERK7 substrate, and that Dishevelled, which has also been shown to regulate ciliogenesis, facilitates ERK7 phosphorylation of CapZIP through binding to both ERK7 and CapZIP. Collectively, these results identify an ERK7/Dishevelled/CapZIP axis that regulates ciliogenesis.

PubMed ID: 25823377
Article link: Nat Commun

Species referenced: Xenopus laevis
Genes referenced: actl6a cetn1 cetn4 dnah9 dvl2 foxj1 gapdh mapk15 pitx2 rcsd1 rsph1 spag6 spef1 tekt2 tph1 tuba1b XB22063824 [provisional]
Antibodies: FLAG Ab1 GFP Ab5 HA Ab9 Mapk15 Ab1 Myc Ab5 Tuba4b Ab4
Morpholinos: foxj1 MO1 mapk15 MO1 mapk15 MO2

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References [+] :
Abe, ERK7 is an autoactivated member of the MAPK family. 2001, Pubmed