Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Naunyn Schmiedebergs Arch Pharmacol
2015 Dec 01;38812:1259-69. doi: 10.1007/s00210-015-1161-y.
Show Gene links
Show Anatomy links
Ikarisoside A inhibits acetylcholine-induced catecholamine secretion and synthesis by suppressing nicotinic acetylcholine receptor-ion channels in cultured bovine adrenal medullary cells.
Li X
,
Toyohira Y
,
Horisita T
,
Satoh N
,
Takahashi K
,
Zhang H
,
Iinuma M
,
Yoshinaga Y
,
Ueno S
,
Tsutsui M
,
Sata T
,
Yanagihara N
.
???displayArticle.abstract???
Ikarisoside A is a natural flavonol glycoside derived from plants of the genus Epimedium, which have been used in Traditional Chinese Medicine as tonics, antirheumatics, and aphrodisiacs. Here, we report the effects of ikarisoside A and three other flavonol glycosides on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. We found that ikarisoside A (1-100 μM), but not icariin, epimedin C, or epimedoside A, concentration-dependently inhibited the secretion of catecholamines induced by acetylcholine, a physiological secretagogue and agonist of nicotinic acetylcholine receptors. Ikarisoside A had little effect on catecholamine secretion induced by veratridine and 56 mM K(+). Ikarisoside A (1-100 μM) also inhibited (22)Na(+) influx and (45)Ca(2+) influx induced by acetylcholine in a concentration-dependent manner similar to that of catecholamine secretion. In Xenopus oocytes expressing α3β4 nicotinic acetylcholine receptors, ikarisoside A (0.1-100 μM) directly inhibited the current evoked by acetylcholine. It also suppressed (14)C-catecholamine synthesis and tyrosine hydroxylase activity induced by acetylcholine at 1-100 μM and 10-100 μM, respectively. The present findings suggest that ikarisoside A inhibits acetylcholine-induced catecholamine secretion and synthesis by suppression of nicotinic acetylcholine receptor-ion channels in bovine adrenal medullary cells.
Akk,
Mutations of the GABA-A receptor alpha1 subunit M1 domain reveal unexpected complexity for modulation by neuroactive steroids.
2008, Pubmed
Akk,
Mutations of the GABA-A receptor alpha1 subunit M1 domain reveal unexpected complexity for modulation by neuroactive steroids.
2008,
Pubmed
Bobrovskaya,
Tyrosine hydroxylase in bovine adrenal chromaffin cells: angiotensin II-stimulated activity and phosphorylation of Ser19, Ser31, and Ser40.
1998,
Pubmed
Campos-Caro,
Neuronal nicotinic acetylcholine receptors on bovine chromaffin cells: cloning, expression, and genomic organization of receptor subunits.
1997,
Pubmed
,
Xenbase
Chen,
Total flavonoid fraction of the Herba epimedii extract suppresses urinary calcium excretion and improves bone properties in ovariectomised mice.
2011,
Pubmed
Choi,
Ikarisoside A inhibits inducible nitric oxide synthase in lipopolysaccharide-stimulated RAW 264.7 cells via p38 kinase and nuclear factor-kappaB signaling pathways.
2008,
Pubmed
Choi,
Inhibition of osteoclastogenic differentiation by Ikarisoside A in RAW 264.7 cells via JNK and NF-kappaB signaling pathways.
2010,
Pubmed
Criado,
Primary structure of an agonist binding subunit of the nicotinic acetylcholine receptor from bovine adrenal chromaffin cells.
1992,
Pubmed
DOUGLAS,
Mechanism of nicotinic action at the adrenal medulla: calcium as a link in stimulus-secretion coupling.
1961,
Pubmed
Dou,
Structure identification of a prenylflavonol glycoside from Epimedium koreanum by electrospray ionization tandem mass spectrometry.
2006,
Pubmed
Douglas,
The mechanism of catecholamine release from the adrenal medulla and the role of calcium in stimulus-secretion coupling.
1963,
Pubmed
García-Guzmán,
alpha-Bungarotoxin-sensitive nicotinic receptors on bovine chromaffin cells: molecular cloning, functional expression and alternative splicing of the alpha 7 subunit.
1995,
Pubmed
,
Xenbase
Hara,
A stress response pathway regulates DNA damage through β2-adrenoreceptors and β-arrestin-1.
2011,
Pubmed
Horishita,
n-Alcohols inhibit voltage-gated Na+ channels expressed in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Kajiwara,
Differential effects of short and prolonged exposure to carvedilol on voltage-dependent Na(+) channels in cultured bovine adrenal medullary cells.
2002,
Pubmed
Kuroda,
Flavonol glycosides from Epimedium sagittatum and their neurite outgrowth activity on PC12h cells.
2000,
Pubmed
Lai,
Icaritin exhibits anti-inflammatory effects in the mouse peritoneal macrophages and peritonitis model.
2013,
Pubmed
Lambert,
Modulation of native and recombinant GABA(A) receptors by endogenous and synthetic neuroactive steroids.
2001,
Pubmed
Liu,
Dual effects of daidzein, a soy isoflavone, on catecholamine synthesis and secretion in cultured bovine adrenal medullary cells.
2007,
Pubmed
Lu,
Where do health benefits of flavonoids come from? Insights from flavonoid targets and their evolutionary history.
2013,
Pubmed
Léna,
Allosteric modulations of the nicotinic acetylcholine receptor.
1993,
Pubmed
López,
Unmasking the functions of the chromaffin cell alpha7 nicotinic receptor by using short pulses of acetylcholine and selective blockers.
1998,
Pubmed
,
Xenbase
NAGATSU,
TYROSINE HYDROXYLASE. THE INITIAL STEP IN NOREPINEPHRINE BIOSYNTHESIS.
1964,
Pubmed
Nijveldt,
Flavonoids: a review of probable mechanisms of action and potential applications.
2001,
Pubmed
Pintado,
The novel Na(+)/Ca(2+) exchange inhibitor KB-R7943 also blocks native and expressed neuronal nicotinic receptors.
2000,
Pubmed
,
Xenbase
Ren,
Neural regeneration: role of traditional Chinese medicine in neurological diseases treatment.
2012,
Pubmed
Shinohara,
Effects of resveratrol, a grape polyphenol, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells.
2007,
Pubmed
,
Xenbase
Toyohira,
Selective blockade of nicotinic acetylcholine receptors by pimobendan, a drug for the treatment of heart failure: reduction of catecholamine secretion and synthesis in adrenal medullary cells.
2005,
Pubmed
Tsutsui,
Correlation of activation of Ca2+/calmodulin-dependent protein kinase II with catecholamine secretion and tyrosine hydroxylase activation in cultured bovine adrenal medullary cells.
1994,
Pubmed
Ueno,
Sites of positive allosteric modulation by neurosteroids on ionotropic gamma-aminobutyric acid receptor subunits.
2004,
Pubmed
,
Xenbase
WEIL-MALHERBE,
The chemical estimation of adrenaline-like substances in blood.
1952,
Pubmed
Wada,
Involvement of Na influx in acetylcholine receptor mediated secretion of catecholamines from cultured bovine adrenal medulla cells.
1984,
Pubmed
Wada,
Influx of 22Na through acetylcholine receptor-associated Na channels: relationship between 22Na influx, 45Ca influx and secretion of catecholamines in cultured bovine adrenal medulla cells.
1985,
Pubmed
Wada,
Modulation by ouabain and diphenylhydantoin of veratridine-induced 22Na influx and its relation to 45Ca influx and the secretion of catecholamines in cultured bovine adrenal medullary cells.
1985,
Pubmed
Yanagihara,
Effects of alpha 2-adrenergic agonists on carbachol-stimulated catecholamine synthesis in cultured bovine adrenal medullary cells.
1987,
Pubmed
Yanagihara,
Muscarinic receptor-mediated increase in cyclic GMP level in isolated bovine adrenal medullary cells.
1979,
Pubmed
Yanagihara,
New insights into the pharmacological potential of plant flavonoids in the catecholamine system.
2014,
Pubmed
Yanagihara,
Phosphorylation of chromogranin A and catecholamine secretion stimulated by elevation of intracellular Ca2+ in cultured bovine adrenal medullary cells.
1996,
Pubmed
Zhang,
Dual effects of nobiletin, a citrus polymethoxy flavone, on catecholamine secretion in cultured bovine adrenal medullary cells.
2010,
Pubmed
,
Xenbase
Zhou,
[Comparison between icariin and genistein in osteogenic activity of marrow stromal cells].
2013,
Pubmed
