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Figure 1. Ba2+ currents recorded in CaV2.1 (A), CaV2.2 (B) and CaV2.2+γ2 (C) channels. A1‐3, B1‐3, C1‐3, depolarization steps for A4‐6, B4‐6 and C4‐6, respectively. A4‐5, B4‐5, C4‐5, currents at 0.1 and at HP, the exact pressure is indicated. A6, B6, C6, superimposed single current traces recorded under normobaric and hyperbaric conditions, generated by identical superimposed depolarization to 10 mV.
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Figure 2. I‐V curves of maximal currents. (A and B) CaV2.1, (C and D) CaV2.2, (E and F) CaV2.2+γ2 channels. (A, C and E) I‐V curve of a single oocyte. (B, D and F) Pooled data from 7–9 (B), 9–12 (D) and 7–10 (F) oocytes exposed to 0.5–5.0 MPa pressure (colour indicated), normalized to maximal current at 0.1 MPa, holding potential is adjusted [ΔV (mV)] so that 0 indicates the potential at which maximal current is obtained (VImax). Statistical significance for each point on the curve is indicated by corresponding colour asterisks (P < 0.05). Dec indicates decompression.
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Figure 3. Channels' conductance at various pressures. (A and B) CaV2.1, (C and D) CaV2.2 and (E and F) CaV2.2+γ2 channels. (A, C and E) Conductance measured in a single oocyte. (B, D and F) Pooled data of the channels, n as stated in Figure 2. Statistical significance for each point on the curve is indicated by corresponding colour asterisks. Holding potential [ΔV (mV)] is expressed as in Figure 2. Dec indicates decompression.
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Figure 4. Voltage‐ and time‐dependent current inactivation (Iend/Imax) at various pressures. (A and B) CaV2.1, (C and D) CaV2.2 and (E and F) CaV2.2+γ2 channels. (A, C and E) Inactivation measured in a single oocyte. (B, D and F) Pooled data of the channels, n as stated in Figure 2. Pressures are colour indicated. Statistical significance for each point on the curve is indicated by corresponding colour asterisks. Holding potential [ΔV (mV)] is expressed as in Figure 2. Dec indicates decompression.
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Figure 5. Time to current peak (TTP) from stimulus onset at various pressures. (A and B) CaV2.1, (C and D) CaV2.2 and (E and F) CaV2.2+γ2 channels. (A, C and E) TTP measured in a single oocyte. (B, D and F) Pooled data of the channels, n as stated in Figure 2. Pressures are colour indicated. Statistical significance for each point on the curve is indicated by corresponding colour asterisks. Holding potential [ΔV (mV)] is expressed as in Figure 2. Dec indicates decompression.
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Figure 6. Time constant of current activation (τRise) at various pressures. (A and B) CaV2.1, (C and D) CaV2.2 and (E and F) CaV2.2+γ2 channels. (A, C and E) τRise measured in a single oocyte. (B, D and F) Pooled data of τRise, n as stated in Figure 2. Pressures are colour indicated. Statistical significance for each point on the curve is indicated by corresponding colour asterisks. Holding potential is expressed [ΔV (mV)] as in Figure 2. Dec indicates decompression.
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Figure 7. Fast time constant of voltage‐ and time‐dependent current inactivation (τDecay Fast). (A and B) CaV2.1, (C and D) CaV2.2, (E and F) CaV2.2+γ2 channels. (A, C and E) τDecay Fast measured in a single oocyte. (B, D and F) Pooled data of the channels, n as stated in Figure 2. Pressures are colour indicated. Statistical significance for each point on the curve is indicated by corresponding colour asterisks. Holding potential [ΔV (mV)] is expressed as in Figure 2. Dec indicates decompression.
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Figure 8. Slow time constant of voltage‐ and time‐dependent current inactivation (τDecay Slow). (A and B) CaV2.1, (C and D) CaV2.2 and (E and F) CaV2.2+γ2 channels. (A, C and E) τDecay Slow measured in a single oocyte. (B, D and F) Pooled data of the channels, n as stated in Figure 2. Pressures are colour indicated. Statistical significance for each point on the curve is indicated by corresponding colour asterisks. Holding potential [ΔV (mV)] is expressed as in Figure 2. Dec indicates decompression.
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Figure 9. Tail current time constant (τTail) at various pressures. (A and B) CaV2.1, (C and D) CaV2.2 and (E and F) CaV2.2+γ2 channels. (A, C and E) τTail measured in a single oocyte. (B, D and F) Pooled data of τTail, n as stated in Figure 2. Pressures are colour indicated. Statistical significance for each point on the curve is indicated by corresponding colour asterisks. Holding potential [ΔV (mV)] is expressed as in Figure 2. Dec indicates decompression.
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