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XB-ART-52493
PLoS One 2016 Aug 16;118:e0161236. doi: 10.1371/journal.pone.0161236.
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Small GTPases Rab8a and Rab11a Are Dispensable for Rhodopsin Transport in Mouse Photoreceptors.

Ying G , Gerstner CD , Frederick JM , Boye SL , Hauswirth WW , Baehr W .


Abstract
Rab11a and Rab8a are ubiquitous small GTPases shown as required for rhodopsin transport in Xenopus laevis and zebrafish photoreceptors by dominant negative (dn) disruption of function. Here, we generated retina-specific Rab11a (retRab11a) and Rab8a (retRab8a) single and double knockout mice to explore the consequences in mouse photoreceptors. Rhodopsin and other outer segment (OS) membrane proteins targeted correctly to OS and electroretinogram (ERG) responses in all three mutant mouse lines were indistinguishable from wild-type (WT). Further, AAV (adeno-associated virus)-mediated expression of dnRab11b in retRab11a-/- retina, or expression of dnRab8b in retRab8a-/- retina did not cause OS protein mislocalization. Finally, a retRab8a-/- retina injected at one month of age with AAVs expressing dnRab11a, dnRab11b, dnRab8b, and dnRab10 (four dn viruses on Rab8a-/- background) and harvested three months later exhibited normal OS protein localization. In contrast to results obtained with dnRab GTPases in Xenopus and zebrafish, mouse Rab11a and Rab8a are dispensable for proper rhodopsin and outer segment membrane protein targeting. Absence of phenotype after expression of four dn Rab GTPases in a Rab8a-/- retina suggests that Rab8b and Rab11b paralogs maybe dispensable as well. Our data thus demonstrate significant interspecies variation in photoreceptor membrane protein and rhodopsin trafficking.

PubMed ID: 27529348
PMC ID: PMC4987053
Article link: PLoS One
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: arrb2 atp2b1 calb1 cdkn1a erg gnat1 myc prom1 rab11a rab11b.1 rab8a rab8b rho slc17a7 zic1


Article Images: [+] show captions
References [+] :
Babbey, Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells. 2006, Pubmed