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Cell Mol Life Sci
2013 Dec 01;7023:4603-16. doi: 10.1007/s00018-013-1403-4.
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Light-dependent phosphorylation of Bardet-Biedl syndrome 5 in photoreceptor cells modulates its interaction with arrestin1.
Smith TS
,
Spitzbarth B
,
Li J
,
Dugger DR
,
Stern-Schneider G
,
Sehn E
,
Bolch SN
,
McDowell JH
,
Tipton J
,
Wolfrum U
,
Smith WC
.
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Arrestins are dynamic proteins that move between cell compartments triggered by stimulation of G-protein-coupled receptors. Even more dynamically in vertebrate photoreceptors, arrestin1 (Arr1) moves between the inner and outer segments according to the light conditions. Previous studies have shown that the light-driven translocation of Arr1 in rod photoreceptors is initiated by rhodopsin through a phospholipase C/protein kinase C (PKC) signaling cascade. The purpose of this study is to identify the PKC substrate that regulates the translocation of Arr1. Mass spectrometry was used to identify the primary phosphorylated proteins in extracts prepared from PKC-stimulated mouse eye cups, confirming the finding with in vitro phosphorylation assays. Our results show that Bardet-Biedl syndrome 5 (BBS5) is the principal protein phosphorylated either by phorbol ester stimulation or by light stimulation of PKC. Via immunoprecipitation of BBS5 in rod outer segments, Arr1 was pulled down; phosphorylation of BBS5 reduced this co-precipitation of Arr1. Immunofluorescence and immunoelectron microscopy showed that BBS5 principally localizes along the axonemes of rods and cones, but also in photoreceptor inner segments, and synaptic regions. Our principal findings in this study are threefold. First, we demonstrate that BBS5 is post-translationally regulated by phosphorylation via PKC, an event that is triggered by light in photoreceptor cells. Second, we find a direct interaction between BBS5 and Arr1, an interaction that is modulated by phosphorylation of BBS5. Finally, we show that BBS5 is distributed along the photoreceptor axoneme, co-localizing with Arr1 in the dark. These findings suggest a role for BBS5 in regulating light-dependent translocation of Arr1 and a model describing its role in Arr1 translocation is proposed.
Ahmed,
Ubiquitin ligase parkin promotes Mdm2-arrestin interaction but inhibits arrestin ubiquitination.
2011,
Pubmed
Brann,
Diurnal expression of transducin mRNA and translocation of transducin in rods of rat retina.
1987,
Pubmed
Broekhuyse,
Light induced shift and binding of S-antigen in retinal rods.
1985,
Pubmed
Calvert,
Diffusion of a soluble protein, photoactivatable GFP, through a sensory cilium.
2010,
Pubmed
,
Xenbase
Crusius,
Enhancement of EGF- and PMA-mediated MAP kinase activation in cells expressing the human papillomavirus type 16 E5 protein.
1997,
Pubmed
Elias,
Temporal kinetics of the light/dark translocation and compartmentation of arrestin and alpha-transducin in mouse photoreceptor cells.
2004,
Pubmed
Gilliam,
Three-dimensional architecture of the rod sensory cilium and its disruption in retinal neurodegeneration.
2012,
Pubmed
Hambleton,
Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages.
1996,
Pubmed
Hanson,
Visual arrestin binding to microtubules involves a distinct conformational change.
2006,
Pubmed
Hanson,
Each rhodopsin molecule binds its own arrestin.
2007,
Pubmed
Hanson,
Arrestin mobilizes signaling proteins to the cytoskeleton and redirects their activity.
2007,
Pubmed
Huang,
Visual Arrestin 1 acts as a modulator for N-ethylmaleimide-sensitive factor in the photoreceptor synapse.
2010,
Pubmed
Jin,
The BBSome.
2009,
Pubmed
Jin,
The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.
2010,
Pubmed
Johnson,
PKC-dependent activation of sphingosine kinase 1 and translocation to the plasma membrane. Extracellular release of sphingosine-1-phosphate induced by phorbol 12-myristate 13-acetate (PMA).
2002,
Pubmed
Kühn,
Light-induced binding of 48-kDa protein to photoreceptor membranes is highly enhanced by phosphorylation of rhodopsin.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Maerker,
A novel Usher protein network at the periciliary reloading point between molecular transport machineries in vertebrate photoreceptor cells.
2008,
Pubmed
,
Xenbase
McDowell,
Sulfhydryl reactivity demonstrates different conformational states for arrestin, arrestin activated by a synthetic phosphopeptide, and constitutively active arrestin.
1999,
Pubmed
McGinnis,
Cytoskeleton participation in subcellular trafficking of signal transduction proteins in rod photoreceptor cells.
2002,
Pubmed
,
Xenbase
Mockel,
Retinal dystrophy in Bardet-Biedl syndrome and related syndromic ciliopathies.
2011,
Pubmed
Nachury,
A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis.
2007,
Pubmed
Nair,
Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors.
2004,
Pubmed
Najafi,
Steric volume exclusion sets soluble protein concentrations in photoreceptor sensory cilia.
2012,
Pubmed
,
Xenbase
Orisme,
Light-dependent translocation of arrestin in rod photoreceptors is signaled through a phospholipase C cascade and requires ATP.
2010,
Pubmed
,
Xenbase
Peet,
Quantification of the cytoplasmic spaces of living cells with EGFP reveals arrestin-EGFP to be in disequilibrium in dark adapted rod photoreceptors.
2004,
Pubmed
,
Xenbase
Peterson,
Arrestin migrates in photoreceptors in response to light: a study of arrestin localization using an arrestin-GFP fusion protein in transgenic frogs.
2003,
Pubmed
,
Xenbase
Peterson,
A role for cytoskeletal elements in the light-driven translocation of proteins in rod photoreceptors.
2005,
Pubmed
,
Xenbase
Philp,
Light-stimulated protein movement in rod photoreceptor cells of the rat retina.
1987,
Pubmed
Reidel,
The translocation of signaling molecules in dark adapting mammalian rod photoreceptor cells is dependent on the cytoskeleton.
2008,
Pubmed
Roepman,
Protein networks and complexes in photoreceptor cilia.
2007,
Pubmed
Satir,
Overview of structure and function of mammalian cilia.
2007,
Pubmed
Sedmak,
Immunoelectron microscopy of vesicle transport to the primary cilium of photoreceptor cells.
2009,
Pubmed
Sedmak,
Intraflagellar transport molecules in ciliary and nonciliary cells of the retina.
2010,
Pubmed
Smith,
Interaction of arrestin with enolase1 in photoreceptors.
2011,
Pubmed
,
Xenbase
Song,
Cone arrestin binding to JNK3 and Mdm2: conformational preference and localization of interaction sites.
2007,
Pubmed
Song,
How does arrestin assemble MAPKs into a signaling complex?
2009,
Pubmed
Song,
Visual and both non-visual arrestins in their "inactive" conformation bind JNK3 and Mdm2 and relocalize them from the nucleus to the cytoplasm.
2006,
Pubmed
Strissel,
Arrestin translocation is induced at a critical threshold of visual signaling and is superstoichiometric to bleached rhodopsin.
2006,
Pubmed
Wei,
The BBSome controls IFT assembly and turnaround in cilia.
2012,
Pubmed
Wolfrum,
Centrin in the photoreceptor cells of mammalian retinae.
1995,
Pubmed
Wolfrum,
Myosin VIIa as a common component of cilia and microvilli.
1998,
Pubmed
Wu,
Arrestin binding to calmodulin: a direct interaction between two ubiquitous signaling proteins.
2006,
Pubmed
Xiao,
Functional specialization of beta-arrestin interactions revealed by proteomic analysis.
2007,
Pubmed
Zhang,
Intrinsic protein-protein interaction-mediated and chaperonin-assisted sequential assembly of stable bardet-biedl syndrome protein complex, the BBSome.
2012,
Pubmed