Tomii C et al. (2017), TPX2 expression is associated with poor surviva...

XB-ART-52939

World J Surg Oncol 2017 Jan 09;151:14. doi: 10.1186/s12957-016-1095-y.

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TPX2 expression is associated with poor survival in gastric cancer.

Tomii C , Inokuchi M , Takagi Y , Ishikawa T , Otsuki S , Uetake H , Kojima K , Kawano T .

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BACKGROUND: Targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein required for microtubule formation in human cells. Several studies have demonstrated that TPX2 is overexpressed in multiple tumor types and promotes tumor growth and metastasis. However, there have been few reports regarding its role in gastric cancer. In this study, we evaluated TPX2 expression and investigated its correlations with gastric cancer clinicopathological features and prognosis. METHODS: Tumor samples were obtained from 290 patients with gastric adenocarcinoma who had undergone gastrectomy. The expression of TPX2 protein was examined using immunohistochemical staining. TPX2 messenger RNA (mRNA) levels were evaluated using real-time quantitative reverse transcription PCR in 19 of the gastric cancer tumors and adjacent normal tissues. RESULTS: The mRNA levels of TPX2 were significantly higher in gastric cancer tissues than in matched adjacent normal tissues (p = 0.004). In the immunohistochemical analysis, TPX2 overexpression was found in 123 (42.4%) of 290 patients. High TPX2 expression was positively associated with age, type of histology, depth of tumor, lymph node metastasis, stage, and remote metastasis or recurrence. High TPX2 expression was significantly associated with poorer disease-specific survival (p = 0.004) and relapse-free interval (p = 0.013). CONCLUSIONS: Our results indicated that high TPX2 expression was associated with tumor progression and poor survival in gastric cancer.

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Species referenced: Xenopus
Genes referenced: pmp22 tpx2

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	Fig. 1. Targeting protein for Xenopus kinesin-like protein 2 (TPX2) mRNA expression in gastric cancerous and adjacent normal tissues. The median TPX2 mRNA level was significantly higher in gastric cancer than in the adjacent normal tissue in 19 patients (p = 0.004)
	Fig. 2. Representative photomicrographs of immunostaining for targeting protein for Xenopus kinesin-like protein 2 (TPX2), demonstrating a negative staining in normal gastric epithelium, b negative staining in primary gastric carcinoma, c positive staining graded as low (<5% of tumor cells stained), and d positive staining graded as high (≥5% of tumor cells stained). TPX2 protein was mainly localized within the nuclei of tumor cells. Images were captured at ×200 magnification
	Fig. 3. Kaplan-Meier curves for the disease-specific survival (DSS) of patients with targeting protein for Xenopus kinesin-like protein 2 (TPX2) expression. Patients with high TPX2 protein expression (n = 123) had significantly poorer DSS than those with low TPX2 expression (n = 167; p = 0.004)
	Fig. 4. Kaplan-Meier curves for the relapse-free interval (RFI) of patients with targeting protein for Xenopus kinesin-like protein 2 (TPX2) expression. Patients with high TPX2 protein expression (n = 123) had significantly poorer RFI than those with low TPX2 expression (n = 167; p = 0.013)
	Fig. 5. Kaplan-Meier curves for disease-specific survival (DSS) after recurrence of patients with low or high levels of targeting protein for Xenopus kinesin-like protein 2 (TPX2) expression. The log-rank test showed no significant difference between DSS and TPX2 expression in patients after recurrence (p = 0.52)

References [+] :

Aguirre-Portolés, Tpx2 controls spindle integrity, genome stability, and tumor development. 2012, Pubmed