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Nat Commun
2016 May 17;7:11608. doi: 10.1038/ncomms11608.
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Congruent pattern of accessibility identifies minimal pore gate in a non-symmetric voltage-gated sodium channel.
Oelstrom K
,
Chanda B
.
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Opening and closing of the central ion-conducting pore in voltage-dependent ion channels is gated by changes in membrane potential. Although a gate residue in the eukaryotic voltage-gated sodium channel has been identified, the minimal molecular determinants of this gate region remain unknown. Here, by measuring the closed- and open-state reactivity of MTSET to substituted cysteines in all the pore-lining helices, we show that the state-dependent accessibility is delineated by four hydrophobic residues at homologous positions in each domain. Introduced cysteines above these sites do not react with intracellular MTSET while the channels are closed and yet are rapidly modified while the channels are open. These findings, in conjunction with state-dependent metal cross-bridging, support the notion that the gate residues in each of the four S6 segments of the eukaryotic sodium channel form an occlusion for ions in the closed state and are splayed open on activation.
Ahern,
The hitchhiker's guide to the voltage-gated sodium channel galaxy.
2016, Pubmed
Ahern,
The hitchhiker's guide to the voltage-gated sodium channel galaxy.
2016,
Pubmed
Armstrong,
Interaction of tetraethylammonium ion derivatives with the potassium channels of giant axons.
1971,
Pubmed
Armstrong,
Ionic pores, gates, and gating currents.
1974,
Pubmed
Armstrong,
Time course of TEA(+)-induced anomalous rectification in squid giant axons.
1966,
Pubmed
Bagnéris,
Role of the C-terminal domain in the structure and function of tetrameric sodium channels.
2013,
Pubmed
Bass,
Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel.
2002,
Pubmed
Brett,
A method for the rapid exchange of solutions bathing excised membrane patches.
1986,
Pubmed
Cahalan,
Local anesthetic block of sodium channels in normal and pronase-treated squid giant axons.
1978,
Pubmed
Catterall,
From ionic currents to molecular mechanisms: the structure and function of voltage-gated sodium channels.
2000,
Pubmed
Chanda,
Tracking voltage-dependent conformational changes in skeletal muscle sodium channel during activation.
2002,
Pubmed
,
Xenbase
Chiang,
Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses.
2004,
Pubmed
Contreras,
Gating at the selectivity filter in cyclic nucleotide-gated channels.
2008,
Pubmed
Finol-Urdaneta,
Sodium channel selectivity and conduction: prokaryotes have devised their own molecular strategy.
2014,
Pubmed
Flynn,
A cysteine scan of the inner vestibule of cyclic nucleotide-gated channels reveals architecture and rearrangement of the pore.
2003,
Pubmed
Hirschberg,
Transfer of twelve charges is needed to open skeletal muscle Na+ channels.
1995,
Pubmed
,
Xenbase
Liao,
Structure of the TRPV1 ion channel determined by electron cryo-microscopy.
2013,
Pubmed
Liu,
Dynamic rearrangement of the outer mouth of a K+ channel during gating.
1996,
Pubmed
Liu,
Gated access to the pore of a voltage-dependent K+ channel.
1997,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
McCusker,
Structure of a bacterial voltage-gated sodium channel pore reveals mechanisms of opening and closing.
2012,
Pubmed
Numa,
Molecular structure of sodium channels.
1986,
Pubmed
Oelstrom,
Evolutionarily conserved intracellular gate of voltage-dependent sodium channels.
2014,
Pubmed
Payandeh,
The crystal structure of a voltage-gated sodium channel.
2011,
Pubmed
Payandeh,
Crystal structure of a voltage-gated sodium channel in two potentially inactivated states.
2012,
Pubmed
Payandeh,
Bacterial voltage-gated sodium channels (BacNa(V)s) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart.
2015,
Pubmed
Raman,
Properties of sodium currents and action potential firing in isolated cerebellar Purkinje neurons.
1999,
Pubmed
Raman,
Inactivation and recovery of sodium currents in cerebellar Purkinje neurons: evidence for two mechanisms.
2001,
Pubmed
Shaya,
Structure of a prokaryotic sodium channel pore reveals essential gating elements and an outer ion binding site common to eukaryotic channels.
2014,
Pubmed
Sunami,
Accessibility of mid-segment domain IV S6 residues of the voltage-gated Na+ channel to methanethiosulfonate reagents.
2004,
Pubmed
,
Xenbase
Wang,
Tryptophan scanning of D1S6 and D4S6 C-termini in voltage-gated sodium channels.
2003,
Pubmed
Xie,
Localization of the activation gate of a voltage-gated Ca2+ channel.
2005,
Pubmed
,
Xenbase
Zhang,
Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel.
2012,
Pubmed
Zhen,
Functional architecture of the inner pore of a voltage-gated Ca2+ channel.
2005,
Pubmed
,
Xenbase
Zhou,
Cysteine scanning and modification reveal major differences between BK channels and Kv channels in the inner pore region.
2011,
Pubmed
,
Xenbase
del Camino,
Tight steric closure at the intracellular activation gate of a voltage-gated K(+) channel.
2001,
Pubmed