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Gene Expr Patterns
2019 Dec 01;34:119056. doi: 10.1016/j.gep.2019.119056.
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Developmental expression patterns of fosl genes in Xenopus tropicalis.
Guo XF
,
Zhang Z
,
Zheng L
,
Zhou YM
,
Wu HY
,
Liang CQ
,
Zhao H
,
Cai DQ
,
Qi XF
.
???displayArticle.abstract??? Fos-like antigens (Fosl) including Fosl1 and Fosl2 exclusively heterodimerize with Jun members to form AP-1 complex, thereby participating in various cellular progresses including cell cycle regulation. However, expression patterns of these two genes during embryonic development remains largely unknown. In the present study, both temporal and spatial expression patterns of fosl1 and fosl2 were examined during embryonic development of Xenopus tropicalis. Real-time quantitative PCR results showed that the expression of the two genes was increased from stage 2 to stage 42. However, expression level of fosl1 is much higher than that of fosl2 at stage 42. Whole-mount in situ hybridization showed that fosl1 was expressed in eyes, branchial arch, notochord, otic vesicle, and liver. However, fosl2 was expressed in lungprimordium from stage 34 to stage 38, in addition to the moderate expression in eyes and branchial arch at stage 42. Thus, the developmental expression patterns of these two fosl genes is different in Xenopus embryos. These results provide a basis for further functional study of these two genes.
Fig. 1. Identification of two fosl genes from Xenopus tropicalis. (A and B) The identity ratio of protein sequence of human Fosl1 (A) and Fosl2 (B) compared with homologs in other vertebrates. M, mouse; r, rat; x,Xenopus tropicalis; d, Danio rerio. (C and D) Phylogenetic analysis of Fosl1(C) and Fosl2 (D) proteins from Xenopus tropicalis, human, mouse, rat,and Danio rerio.
Fig. 2. Temporal expression of fosl genes in early development of X. tropicalis. The mRNA expression of fosl1 and fosl2 genes was analyzed by qPCR using total RNA isolated from X. tropicalis embryos at indicated developmental stages. Ornithine decarboxylase (odc) was used as the internal standard control. Results are presented as mean ± SEM (n = 4 experiments), ***p < 0.001. St, stage.
Fig. 3. Spatial pattern of fosl1 analyzed by whole mount in situ hybridization. (A and A′) Stage 2, animal view. (B and B′) Stage 8, animal view. (C and C′) Stage 13, dorsal view. (D and D′) Stage 27, dorsal view. (E and E′) Stage 34, lateral view. (F and F′) Stage 38, lateral view. (G and G′) Stage 42, lateral view. Ba, branchial arches; cb, cement gland; fb, forebrain; mb, midbrain; hb, hindbrain; lv, liver; nc, notochord; ov, otic vesicle. A′-G′ indicates the sense probe control.
Fig. 4. Spatial pattern of fosl2analyzed by whole mount in situ hybridization. (A and A′) Stage 2, animal view. (B and B′) Stage 8, animal view. (C and C′) Stage 13, dorsal view. (D and D′) Stage 27, dorsal view. (E and E′) Stage 34, lateral view. (F and F′) Stage 38, lateral view. (G and G′) Stage 42, lateral view. (H) Transverse sections of embryos at the levels illustrated by broken black lines in F. (H′) Large magnification of image shown in H. Ba, branchial arch; nc, notochord. A′-G′ indicates the sense probe control.
fosl1 (FOS-like antigen 1 ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 34, lateral view, anteriorright, dorsal up.
fosl1 (FOS-like antigen 1 ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 42, lateral view, anteriorright, dorsal up.
fosl2 (FOS-like antigen 2 ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 38, lateral view, anteriorright, dorsal up.
fosl2 (FOS-like antigen 2) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 42, lateral view, anteriorright, dorsal up.
