Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-56017
Methods Mol Biol 2019 Jan 01;1999:173-184. doi: 10.1007/978-1-4939-9500-4_10.
Show Gene links Show Anatomy links

Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts.

Wolfe KB , Long DT .


???displayArticle.abstract???
Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and Xenopus egg extracts provides a highly versatile and tractable approach to analyze dynamic protein-DNA interactions with great spatial and temporal detail.

???displayArticle.pubmedLink??? 31127576
???displayArticle.pmcLink??? PMC8091904
???displayArticle.link??? Methods Mol Biol
???displayArticle.grants??? [+]


References [+] :
Bernis, Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays. 2014, Pubmed, Xenbase