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Mol Neurobiol
2017 Aug 01;546:4659-4671. doi: 10.1007/s12035-016-0007-z.
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Lentiviral Delivery of miR-133b Improves Functional Recovery After Spinal Cord Injury in Mice.
Theis T
,
Yoo M
,
Park CS
,
Chen J
,
Kügler S
,
Gibbs KM
,
Schachner M
.
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Based on the observation that microRNA (miRNA) 133b enhances regeneration after spinal cord injury in the adult zebrafish, we investigated whether this miRNA would be beneficial in a mammalian system in vitro and in vivo. We found that infection of cultured neurons with miR-133b promotes neurite outgrowth in vitro on an inhibitory substrate consisting of mixed chondroitin sulfate proteoglycans, when compared to infection with green fluorescent protein (GFP) for control. In vivo, viral infection of the injured adult mouse spinal cord at the time of injury at and in the vicinity of the lesion site enhanced expression of miR-133b. Measurements of locomotor recovery by Basso Mouse Scale (BMS) showed improvement of recovery starting at 4 weeks after injury and virus injection. This improvement was associated with downregulation of the expression levels of Ras homolog gene family member A (RhoA), chondroitin sulfate proteoglycans, and microglia/macrophage marker in the spinal cord as assayed 6 weeks after injury. Potential inhibitory molecules carrying consensus sequences for binding of miR-133b were identified in silico and verified in a reporter assay in vitro showing reductions in expression of RhoA, xylosyltransferase 1 (Xylt1), ephrin receptor A7 (Epha7), and purinergic receptor P2X ligand-gated ion channel 4 (P2RX4). These results encourage targeting miR-133 for therapy.
Apostolova,
Tenascin-R restricts posttraumatic remodeling of motoneuron innervation and functional recovery after spinal cord injury in adult mice.
2006, Pubmed
Apostolova,
Tenascin-R restricts posttraumatic remodeling of motoneuron innervation and functional recovery after spinal cord injury in adult mice.
2006,
Pubmed
Atwal,
PirB is a functional receptor for myelin inhibitors of axonal regeneration.
2008,
Pubmed
Bartel,
Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs.
2004,
Pubmed
Basso,
Basso Mouse Scale for locomotion detects differences in recovery after spinal cord injury in five common mouse strains.
2006,
Pubmed
Beattie,
Mutations in the stumpy gene reveal intermediate targets for zebrafish motor axons.
2000,
Pubmed
Belaich,
Sequencing and characterisation of p74 gene in two isolates of Anticarsia gemmatalis MNPV.
2006,
Pubmed
Bhalala,
microRNA-21 regulates astrocytic response following spinal cord injury.
2012,
Pubmed
Bradbury,
Chondroitinase ABC promotes functional recovery after spinal cord injury.
2002,
Pubmed
Carbajal,
RhoA inactivation enhances endothelial barrier function.
1999,
Pubmed
Carulli,
Composition of perineuronal nets in the adult rat cerebellum and the cellular origin of their components.
2006,
Pubmed
Carè,
MicroRNA-133 controls cardiac hypertrophy.
2007,
Pubmed
Chen,
Adeno-associated virus-mediated L1 expression promotes functional recovery after spinal cord injury.
2007,
Pubmed
Curtis,
Up-regulation of GAP-43 and growth of axons in rat spinal cord after compression injury.
1993,
Pubmed
David,
Macrophage and microglial plasticity in the injured spinal cord.
2015,
Pubmed
De Luca,
Astrocytes and Microglia-Mediated Immune Response in Maladaptive Plasticity is Differently Modulated by NGF in the Ventral Horn of the Spinal Cord Following Peripheral Nerve Injury.
2016,
Pubmed
Dergham,
Rho signaling pathway targeted to promote spinal cord repair.
2002,
Pubmed
Dickendesher,
NgR1 and NgR3 are receptors for chondroitin sulfate proteoglycans.
2012,
Pubmed
Donnelly,
Inflammation and its role in neuroprotection, axonal regeneration and functional recovery after spinal cord injury.
2008,
Pubmed
Drinkut,
Efficient gene therapy for Parkinson's disease using astrocytes as hosts for localized neurotrophic factor delivery.
2012,
Pubmed
Erschbamer,
RhoA, RhoB, RhoC, Rac1, Cdc42, and Tc10 mRNA levels in spinal cord, sensory ganglia, and corticospinal tract neurons and long-lasting specific changes following spinal cord injury.
2005,
Pubmed
Essler,
Pasteurella multocida toxin increases endothelial permeability via Rho kinase and myosin light chain phosphatase.
1998,
Pubmed
Figueroa,
Inhibition of EphA7 up-regulation after spinal cord injury reduces apoptosis and promotes locomotor recovery.
2006,
Pubmed
Fournier,
Rho kinase inhibition enhances axonal regeneration in the injured CNS.
2003,
Pubmed
Fujita,
Axon growth inhibition by RhoA/ROCK in the central nervous system.
2014,
Pubmed
Goldshmit,
Rapamycin increases neuronal survival, reduces inflammation and astrocyte proliferation after spinal cord injury.
2015,
Pubmed
González-Estévez,
Diverse miRNA spatial expression patterns suggest important roles in homeostasis and regeneration in planarians.
2009,
Pubmed
Grimpe,
A novel DNA enzyme reduces glycosaminoglycan chains in the glial scar and allows microtransplanted dorsal root ganglia axons to regenerate beyond lesions in the spinal cord.
2004,
Pubmed
Hordijk,
Vascular-endothelial-cadherin modulates endothelial monolayer permeability.
1999,
Pubmed
Huo,
Inhibition of retinal ganglion cell axonal outgrowth through the Amino-Nogo-A signaling pathway.
2013,
Pubmed
Hutvágner,
A microRNA in a multiple-turnover RNAi enzyme complex.
2002,
Pubmed
Jee,
MicroRNA 486 is a potentially novel target for the treatment of spinal cord injury.
2012,
Pubmed
Jee,
Silencing of miR20a is crucial for Ngn1-mediated neuroprotection in injured spinal cord.
2012,
Pubmed
Ji,
Emerging targets in neuroinflammation-driven chronic pain.
2014,
Pubmed
Johnston,
A microRNA controlling left/right neuronal asymmetry in Caenorhabditis elegans.
2003,
Pubmed
Joshi,
Inhibition of Rho-kinase differentially affects axon regeneration of peripheral motor and sensory nerves.
2015,
Pubmed
Kamei,
Neural progenitor cells promote corticospinal axon growth in organotypic co-cultures.
2004,
Pubmed
Keene,
Ribonucleoprotein infrastructure regulating the flow of genetic information between the genome and the proteome.
2001,
Pubmed
Kubo,
Rho-ROCK inhibitors as emerging strategies to promote nerve regeneration.
2007,
Pubmed
Lee,
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.
1993,
Pubmed
Leverrier,
Requirement for Rho GTPases and PI 3-kinases during apoptotic cell phagocytosis by macrophages.
2001,
Pubmed
Li,
Hypoxia induced amoeboid microglial cell activation in postnatal rat brain is mediated by ATP receptor P2X4.
2011,
Pubmed
Lê,
Sensory presynaptic and widespread somatodendritic immunolocalization of central ionotropic P2X ATP receptors.
1998,
Pubmed
Matsuda,
Electroporation and RNA interference in the rodent retina in vivo and in vitro.
2004,
Pubmed
McGee,
The Nogo-66 receptor: focusing myelin inhibition of axon regeneration.
2003,
Pubmed
McKerracher,
Spinal cord repair: strategies to promote axon regeneration.
2001,
Pubmed
Morgenstern,
Chondroitin sulphate proteoglycans in the CNS injury response.
2002,
Pubmed
Persidsky,
Rho-mediated regulation of tight junctions during monocyte migration across the blood-brain barrier in HIV-1 encephalitis (HIVE).
2006,
Pubmed
Pietri,
Transcriptional mechanisms of EphA7 gene expression in the developing cerebral cortex.
2012,
Pubmed
Pixley,
BCL6 suppresses RhoA activity to alter macrophage morphology and motility.
2005,
Pubmed
Qiu,
Spinal axon regeneration induced by elevation of cyclic AMP.
2002,
Pubmed
Ren,
Managing inflammation after spinal cord injury through manipulation of macrophage function.
2013,
Pubmed
Ridley,
Rho proteins, PI 3-kinases, and monocyte/macrophage motility.
2001,
Pubmed
Rogers,
Distribution of the receptor EphA7 and its ligands in development of the mouse nervous system.
1999,
Pubmed
Salter,
Sublime microglia: expanding roles for the guardians of the CNS.
2014,
Pubmed
Schnell,
Cytokine-induced acute inflammation in the brain and spinal cord.
1999,
Pubmed
Schwab,
Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
2005,
Pubmed
Schwab,
Increasing plasticity and functional recovery of the lesioned spinal cord.
2002,
Pubmed
Schwab,
Spinal cord injury-induced lesional expression of the repulsive guidance molecule (RGM).
2005,
Pubmed
Shi,
Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.
2009,
Pubmed
Steward,
False resurrections: distinguishing regenerated from spared axons in the injured central nervous system.
2003,
Pubmed
Stoolmiller,
Biosynthesis of the chondroitin sulfate proteoglycan. Purification and properties of xylosyltransferase.
1972,
Pubmed
Trivedi,
Inflammation and Spinal Cord Injury: Infiltrating Leukocytes as Determinants of Injury and Repair Processes.
2006,
Pubmed
Willson,
Upregulation of EphA receptor expression in the injured adult rat spinal cord.
2002,
Pubmed
Wälchli,
Nogo-A is a negative regulator of CNS angiogenesis.
2013,
Pubmed
Xin,
MiR-133b promotes neural plasticity and functional recovery after treatment of stroke with multipotent mesenchymal stromal cells in rats via transfer of exosome-enriched extracellular particles.
2013,
Pubmed
Yoo,
Analysis of human embryonic stem cells with regulatable expression of the cell adhesion molecule l1 in regeneration after spinal cord injury.
2014,
Pubmed
You,
Passive immunization with tenascin-R (TN-R) polyclonal antibody promotes axonal regeneration and functional recovery after spinal cord injury in rats.
2012,
Pubmed
Yu,
MicroRNA miR-133b is essential for functional recovery after spinal cord injury in adult zebrafish.
2011,
Pubmed
,
Xenbase
Zhu,
In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations.
2007,
Pubmed
