Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
J Cell Biol 1985 Apr 01;1004:1284-94. doi: 10.1083/jcb.100.4.1284.
Show Gene links Show Anatomy links

Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells.

Buckley K , Kelly RB .

Several types of cells store proteins in secretory vesicles from which they are released by an appropriate stimulus. It might be expected that the secretory vesicles in different cell types use similar molecular machinery. Here we describe a transmembrane glycoprotein (Mr approximately 100,000) that is present in secretory vesicles in all neurons and endocrine cells studied, in species from elasmobranch fish to mammals, and in neural and endocrine cell lines. It was detected by cross-reactivity with monoclonal antibodies raised to highly purified cholinergic synaptic vesicles from the electric organ of fish. By immunoprecipitation of intact synaptic vesicles and electron microscopic immunoperoxidase labeling, we have shown that the antigenic determinant is on the cytoplasmic face of the synaptic vesicles. However, the electrophoretic mobility of the antigen synthesized in the presence of tunicamycin is reduced to Mr approximately 62,000, which suggests that the antigen is glycosylated and must therefore span the vesicle membrane.

PubMed ID: 2579958
PMC ID: PMC2113776
Article link: J Cell Biol

Antibodies: Sv2a Ab1

References [+] :
Bishop, Neuron specific enolase: a common marker for the endocrine cells and innervation of the gut and pancreas. 1982, Pubmed