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Raj S
,
Kyono Y
,
Sifuentes CJ
,
Arellanes-Licea EDC
,
Subramani A
,
Denver RJ
.
???displayArticle.abstract??? Thyroid hormone (T3) plays pivotal roles in vertebrate development, acting via nuclear T3 receptors (TRs) that regulate gene transcription by promoting post-translational modifications to histones. Methylation of cytosine residues in deoxyribonucleic acid (DNA) also modulates gene transcription, and our recent finding of predominant DNA demethylation in the brain of Xenopus tadpoles at metamorphosis, a T3-dependent developmental process, caused us to hypothesize that T3 induces these changes in vivo. Treatment of premetamorphic tadpoles with T3 for 24 or 48 hours increased immunoreactivity in several brain regions for the DNA demethylation intermediates 5-hydroxymethylcytosine (5-hmC) and 5-carboxylcytosine, and the methylcytosine dioxygenase ten-eleven translocation 3 (TET3). Thyroid hormone treatment induced locus-specific DNA demethylation in proximity to known T3 response elements within the DNA methyltransferase 3a and Krüppel-like factor 9 genes, analyzed by 5-hmC immunoprecipitation and methylation sensitive restriction enzyme digest. Chromatin-immunoprecipitation (ChIP) assay showed that T3 induced TET3 recruitment to these loci. Furthermore, the messenger ribonucleic acid for several genes encoding DNA demethylation enzymes were induced by T3 in a time-dependent manner in tadpolebrain. A TR ChIP-sequencing experiment identified putative TR binding sites at several of these genes, and we provide multiple lines of evidence to support that tet2 contains a bona fide T3 response element. Our findings show that T3 can promote DNA demethylation in developing tadpolebrain, in part by promoting TET3 recruitment to discrete genomic regions, and by inducing genes that encode DNA demethylation enzymes.
Auclair,
Mechanisms of DNA methylation and demethylation in mammals.
2012, Pubmed
Auclair,
Mechanisms of DNA methylation and demethylation in mammals.
2012,
Pubmed
Bagamasbad,
Deciphering the regulatory logic of an ancient, ultraconserved nuclear receptor enhancer module.
2015,
Pubmed
,
Xenbase
Bird,
A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNA.
1985,
Pubmed
Bochtler,
DNA demethylation pathways: Additional players and regulators.
2017,
Pubmed
Bogdanovic,
Temporal uncoupling of the DNA methylome and transcriptional repression during embryogenesis.
2011,
Pubmed
,
Xenbase
Bogdanović,
Active DNA demethylation at enhancers during the vertebrate phylotypic period.
2016,
Pubmed
,
Xenbase
Bogdanović,
DNA methylation and the preservation of cell identity.
2017,
Pubmed
Brown,
Amphibian metamorphosis.
2007,
Pubmed
,
Xenbase
Buchholz,
Xenopus metamorphosis as a model to study thyroid hormone receptor function during vertebrate developmental transitions.
2017,
Pubmed
,
Xenbase
Buchholz,
A dominant-negative thyroid hormone receptor blocks amphibian metamorphosis by retaining corepressors at target genes.
2003,
Pubmed
,
Xenbase
Cheng,
Molecular aspects of thyroid hormone actions.
2010,
Pubmed
Cokus,
Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning.
2008,
Pubmed
Crespi,
Leptin (ob gene) of the South African clawed frog Xenopus laevis.
2006,
Pubmed
,
Xenbase
Denver,
The molecular basis of thyroid hormone-dependent central nervous system remodeling during amphibian metamorphosis.
1998,
Pubmed
Denver,
Identification of a thyroid hormone response element in the mouse Kruppel-like factor 9 gene to explain its postnatal expression in the brain.
2009,
Pubmed
Denver,
Neuroendocrinology of amphibian metamorphosis.
2013,
Pubmed
Edwards,
DNA methylation and DNA methyltransferases.
2017,
Pubmed
Escriva,
The evolution of the nuclear receptor superfamily.
2004,
Pubmed
Feng,
Conservation and divergence of methylation patterning in plants and animals.
2010,
Pubmed
Figueroa,
Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.
2010,
Pubmed
Furlow,
The transcription factor basic transcription element-binding protein 1 is a direct thyroid hormone response gene in the frog Xenopus laevis.
2002,
Pubmed
,
Xenbase
Guan,
Methylcytosine dioxygenase TET3 interacts with thyroid hormone nuclear receptors and stabilizes their association to chromatin.
2017,
Pubmed
Guo,
Neuronal activity modifies the DNA methylation landscape in the adult brain.
2011,
Pubmed
Hackett,
Germline DNA demethylation dynamics and imprint erasure through 5-hydroxymethylcytosine.
2013,
Pubmed
Hassan,
Regulation of Active DNA Demethylation through RAR-Mediated Recruitment of a TET/TDG Complex.
2017,
Pubmed
He,
Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA.
2011,
Pubmed
Heimeier,
Participation of Brahma-related gene 1 (BRG1)-associated factor 57 and BRG1-containing chromatin remodeling complexes in thyroid hormone-dependent gene activation during vertebrate development.
2008,
Pubmed
,
Xenbase
Hon,
Epigenetic memory at embryonic enhancers identified in DNA methylation maps from adult mouse tissues.
2013,
Pubmed
Jjingo,
On the presence and role of human gene-body DNA methylation.
2012,
Pubmed
Jones,
The DNA methylation paradox.
1999,
Pubmed
Kasai,
Exposure to 3,3',5-triiodothyronine affects histone and RNA polymerase II modifications, but not DNA methylation status, in the regulatory region of the Xenopus laevis thyroid hormone receptor βΑ gene.
2015,
Pubmed
,
Xenbase
Kikuyama,
Aspects of amphibian metamorphosis: hormonal control.
1993,
Pubmed
,
Xenbase
Kohli,
TET enzymes, TDG and the dynamics of DNA demethylation.
2013,
Pubmed
Krain,
Developmental expression and hormonal regulation of glucocorticoid and thyroid hormone receptors during metamorphosis in Xenopus laevis.
2004,
Pubmed
,
Xenbase
Kyono,
Developmental and Thyroid Hormone Regulation of the DNA Methyltransferase 3a Gene in Xenopus Tadpoles.
2016,
Pubmed
,
Xenbase
Kyono,
Liganded Thyroid Hormone Receptors Transactivate the DNA Methyltransferase 3a Gene in Mouse Neuronal Cells.
2016,
Pubmed
Kyono,
DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brain.
2020,
Pubmed
,
Xenbase
Laudet,
The origins and evolution of vertebrate metamorphosis.
2011,
Pubmed
,
Xenbase
Law,
Establishing, maintaining and modifying DNA methylation patterns in plants and animals.
2010,
Pubmed
Lazar,
Thyroid hormone action: a binding contract.
2003,
Pubmed
Lebel,
Overexpression of the beta 1 thyroid receptor induces differentiation in neuro-2a cells.
1994,
Pubmed
Lecroisey,
The cephalochordate amphioxus: a key to reveal the secrets of nuclear receptor evolution.
2012,
Pubmed
Li,
Role for DNA methylation in genomic imprinting.
1993,
Pubmed
Li,
Tet proteins influence the balance between neuroectodermal and mesodermal fate choice by inhibiting Wnt signaling.
2016,
Pubmed
Li,
DNA methylation in mammals.
2014,
Pubmed
Lian,
Loss of 5-hydroxymethylcytosine is an epigenetic hallmark of melanoma.
2012,
Pubmed
Lister,
Global epigenomic reconfiguration during mammalian brain development.
2013,
Pubmed
Lister,
Human DNA methylomes at base resolution show widespread epigenomic differences.
2009,
Pubmed
Lu,
TET family proteins: oxidation activity, interacting molecules, and functions in diseases.
2015,
Pubmed
Machuca,
Analysis of structure and expression of the Xenopus thyroid hormone receptor-beta gene to explain its autoinduction.
1995,
Pubmed
,
Xenbase
Maiti,
Thymine DNA glycosylase can rapidly excise 5-formylcytosine and 5-carboxylcytosine: potential implications for active demethylation of CpG sites.
2011,
Pubmed
Marín,
Basal ganglia organization in amphibians: chemoarchitecture.
1998,
Pubmed
,
Xenbase
Matsuura,
Liganded thyroid hormone receptor induces nucleosome removal and histone modifications to activate transcription during larval intestinal cell death and adult stem cell development.
2012,
Pubmed
,
Xenbase
Mellén,
MeCP2 binds to 5hmC enriched within active genes and accessible chromatin in the nervous system.
2012,
Pubmed
Moore,
DNA methylation and its basic function.
2013,
Pubmed
Nestor,
Hydroxymethylated DNA immunoprecipitation (hmeDIP).
2014,
Pubmed
Nestor,
5-Hydroxymethylcytosine Remodeling Precedes Lineage Specification during Differentiation of Human CD4(+) T Cells.
2016,
Pubmed
Paulsen,
DNA methylation in genomic imprinting, development, and disease.
2001,
Pubmed
Porterfield,
The role of thyroid hormones in prenatal and neonatal neurological development--current perspectives.
1993,
Pubmed
Raj,
Thyroid Hormone Induces DNA Demethylation in Xenopus Tadpole Brain.
2020,
Pubmed
,
Xenbase
Ranjan,
Transcriptional repression of Xenopus TR beta gene is mediated by a thyroid hormone response element located near the start site.
1994,
Pubmed
,
Xenbase
Ross,
TET enzymes, DNA demethylation and pluripotency.
2019,
Pubmed
Samuels,
Depletion of L-3,5,3'-triiodothyronine and L-thyroxine in euthyroid calf serum for use in cell culture studies of the action of thyroid hormone.
1979,
Pubmed
Schäfer,
Gadd45 proteins: key players of repair-mediated DNA demethylation.
2013,
Pubmed
Shi,
Thyroid hormone-dependent regulation of the intestinal fatty acid-binding protein gene during amphibian metamorphosis.
1994,
Pubmed
,
Xenbase
Shi,
Unliganded thyroid hormone receptor regulates metamorphic timing via the recruitment of histone deacetylase complexes.
2013,
Pubmed
,
Xenbase
Shi,
New Insights into 5hmC DNA Modification: Generation, Distribution and Function.
2017,
Pubmed
Song,
Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine.
2011,
Pubmed
Suzuki,
DNA methylation landscapes: provocative insights from epigenomics.
2008,
Pubmed
Szulwach,
5-hmC-mediated epigenetic dynamics during postnatal neurodevelopment and aging.
2011,
Pubmed
Tahiliani,
Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1.
2009,
Pubmed
Tata,
Autoinduction of nuclear receptor genes and its significance.
1993,
Pubmed
,
Xenbase
Taylor,
Stable 5-Hydroxymethylcytosine (5hmC) Acquisition Marks Gene Activation During Chondrogenic Differentiation.
2016,
Pubmed
Tsagaratou,
Dissecting the dynamic changes of 5-hydroxymethylcytosine in T-cell development and differentiation.
2014,
Pubmed
Tuinhof,
Distribution of pro-opiomelanocortin and its peptide end products in the brain and hypophysis of the aquatic toad, Xenopus laevis.
1998,
Pubmed
,
Xenbase
Wen,
Thyroid Hormone Receptor Alpha Is Required for Thyroid Hormone-Dependent Neural Cell Proliferation During Tadpole Metamorphosis.
2019,
Pubmed
,
Xenbase
Wen,
Thyroid Hormone Receptor α Controls Developmental Timing and Regulates the Rate and Coordination of Tissue-Specific Metamorphosis in Xenopus tropicalis.
2017,
Pubmed
,
Xenbase
Wen,
Unliganded thyroid hormone receptor α controls developmental timing in Xenopus tropicalis.
2015,
Pubmed
,
Xenbase
Wong,
Determinants of chromatin disruption and transcriptional regulation instigated by the thyroid hormone receptor: hormone-regulated chromatin disruption is not sufficient for transcriptional activation.
1997,
Pubmed
,
Xenbase
Wong,
A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor.
1995,
Pubmed
,
Xenbase
Wu,
Reversing DNA methylation: mechanisms, genomics, and biological functions.
2014,
Pubmed
Wu,
Decoding the role of TET family dioxygenases in lineage specification.
2018,
Pubmed
Wu,
TET-mediated active DNA demethylation: mechanism, function and beyond.
2017,
Pubmed
Yao,
Distribution and corticosteroid regulation of glucocorticoid receptor in the brain of Xenopus laevis.
2008,
Pubmed
,
Xenbase
Yao,
Distribution and acute stressor-induced activation of corticotrophin-releasing hormone neurones in the central nervous system of Xenopus laevis.
2004,
Pubmed
,
Xenbase
Yao,
Structural and functional conservation of vertebrate corticotropin-releasing factor genes: evidence for a critical role for a conserved cyclic AMP response element.
2007,
Pubmed
,
Xenbase
Zeng,
DNA Methylation Reprogramming during Mammalian Development.
2019,
Pubmed
