Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Heliyon
2020 Oct 01;610:e05140. doi: 10.1016/j.heliyon.2020.e05140.
Show Gene links
Show Anatomy links
A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids.
Steinberg X
,
Galpin J
,
Nasir G
,
Sepúlveda RV
,
Ladron de Guevara E
,
Gonzalez-Nilo F
,
Islas LD
,
Ahern CA
,
Brauchi SE
.
???displayArticle.abstract???
The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of a novel orthogonal aminoacyl-tRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells.
Bae,
Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins.
2003, Pubmed
Bae,
Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins.
2003,
Pubmed
Baumann,
Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater.
2019,
Pubmed
Baumann,
Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine.
2019,
Pubmed
Bonnefond,
Evolution of the tRNA(Tyr)/TyrRS aminoacylation systems.
2005,
Pubmed
Chatterjee,
A genetically encoded fluorescent probe in mammalian cells.
2013,
Pubmed
Chin,
An expanded eukaryotic genetic code.
2003,
Pubmed
Drabkin,
Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.
1996,
Pubmed
Giepmans,
The fluorescent toolbox for assessing protein location and function.
2006,
Pubmed
Hino,
Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code.
2006,
Pubmed
Kalstrup,
Dynamics of internal pore opening in K(V) channels probed by a fluorescent unnatural amino acid.
2013,
Pubmed
,
Xenbase
Kiga,
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system.
2002,
Pubmed
Kobayashi,
Structural snapshots of the KMSKS loop rearrangement for amino acid activation by bacterial tyrosyl-tRNA synthetase.
2005,
Pubmed
Kobayashi,
Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion.
2005,
Pubmed
Lepthien,
Azatryptophans endow proteins with intrinsic blue fluorescence.
2008,
Pubmed
Merkel,
Blue fluorescent amino acids as in vivo building blocks for proteins.
2010,
Pubmed
Pantoja,
Single-molecule imaging of a fluorescent unnatural amino acid incorporated into nicotinic receptors.
2009,
Pubmed
,
Xenbase
Rauch,
Improved Incorporation of Noncanonical Amino Acids by an Engineered tRNA(Tyr) Suppressor.
2016,
Pubmed
Sakamoto,
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.
2002,
Pubmed
Sakata,
Voltage-dependent motion of the catalytic region of voltage-sensing phosphatase monitored by a fluorescent amino acid.
2016,
Pubmed
,
Xenbase
Shen,
Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons.
2011,
Pubmed
Steinberg,
Conformational dynamics in TRPV1 channels reported by an encoded coumarin amino acid.
2017,
Pubmed
Stokes,
Enhancing the utility of unnatural amino acid synthetases by manipulating broad substrate specificity.
2009,
Pubmed
Summerer,
A genetically encoded fluorescent amino acid.
2006,
Pubmed
Trott,
AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading.
2010,
Pubmed
Tsien,
The green fluorescent protein.
1998,
Pubmed
Wagner,
The use of coumarins as environmentally-sensitive fluorescent probes of heterogeneous inclusion systems.
2009,
Pubmed
Wang,
A genetically encoded fluorescent amino acid.
2006,
Pubmed
Ye,
Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis.
2008,
Pubmed
Zhang,
Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine.
2005,
Pubmed
