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Sci Rep
2019 Oct 02;91:14212. doi: 10.1038/s41598-019-50572-8.
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Generation of Remosomes by the SWI/SNF Chromatin Remodeler Family.
Shukla MS
,
Syed SH
,
Boopathi R
,
Simon EB
,
Nahata S
,
Ramos L
,
Dalkara D
,
Moskalenko C
,
Travers A
,
Angelov D
,
Dimitrov S
,
Hamiche A
,
Bednar J
.
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Chromatin remodelers are complexes able to both alter histone-DNA interactions and to mobilize nucleosomes. The mechanism of their action and the conformation of remodeled nucleosomes remain a matter of debates. In this work we compared the type and structure of the products of nucleosome remodeling by SWI/SNF and ACF complexes using high-resolution microscopy combined with novel biochemical approaches. We find that SWI/SNF generates a multitude of nucleosome-like metastable particles termed "remosomes". Restriction enzyme accessibility assay, DNase I footprinting and AFM experiments reveal perturbed histone-DNA interactions within these particles. Electron cryo-microscopy shows that remosomes adopt a variety of different structures with variable irregular DNA path, similar to those described upon RSC remodeling. Remosome DNA accessibility to restriction enzymes is also markedly increased. We suggest that the generation of remosomes is a common feature of the SWI/SNF family remodelers. In contrast, the ACF remodeler, belonging to ISWI family, only produces repositioned nucleosomes and no evidence for particles associated with extra DNA, or perturbed DNA paths was found. The remosome generation by the SWI/SNF type of remodelers may represent a novel mechanism involved in processes where nucleosomal DNA accessibility is required, such as DNA repair or transcription regulation.
Angelov,
SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays.
2004, Pubmed,
Xenbase
Angelov,
SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays.
2004,
Pubmed
,
Xenbase
Angelov,
Nucleolin is a histone chaperone with FACT-like activity and assists remodeling of nucleosomes.
2006,
Pubmed
,
Xenbase
Angelov,
The histone octamer is invisible when NF-kappaB binds to the nucleosome.
2004,
Pubmed
,
Xenbase
Arents,
The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix.
1991,
Pubmed
Batsché,
The human SWI/SNF subunit Brm is a regulator of alternative splicing.
2006,
Pubmed
Beato,
Transcription factor access to chromatin.
1997,
Pubmed
Becker,
Nucleosome sliding: facts and fiction.
2002,
Pubmed
Boulard,
Histone variant nucleosomes: structure, function and implication in disease.
2007,
Pubmed
Chaban,
Structure of a RSC-nucleosome complex and insights into chromatin remodeling.
2008,
Pubmed
Chai,
Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair.
2005,
Pubmed
Clapier,
The biology of chromatin remodeling complexes.
2009,
Pubmed
Côté,
Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding.
1998,
Pubmed
Dimitrov,
The globular domain of histone H5 is internally located in the 30 nm chromatin fiber: an immunochemical study.
1987,
Pubmed
Doyen,
Dissection of the unusual structural and functional properties of the variant H2A.Bbd nucleosome.
2006,
Pubmed
,
Xenbase
Dror,
The Swi/Snf chromatin remodeling complex is required for ribosomal DNA and telomeric silencing in Saccharomyces cerevisiae.
2004,
Pubmed
Duband-Goulet,
Methods for chromatin assembly and remodeling.
2004,
Pubmed
Dubochet,
Cryo-electron microscopy of vitrified specimens.
1988,
Pubmed
Fazzio,
Widespread collaboration of Isw2 and Sin3-Rpd3 chromatin remodeling complexes in transcriptional repression.
2001,
Pubmed
Fyodorov,
Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivo.
2004,
Pubmed
Gangaraju,
Mechanisms of ATP dependent chromatin remodeling.
2007,
Pubmed
Havas,
ATP-dependent chromatin remodeling activities.
2001,
Pubmed
Hayes,
Nucleosomes and the chromatin fiber.
2001,
Pubmed
Ito,
Drosophila NAP-1 is a core histone chaperone that functions in ATP-facilitated assembly of regularly spaced nucleosomal arrays.
1996,
Pubmed
Ito,
ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor.
1997,
Pubmed
Lorch,
Histone octamer transfer by a chromatin-remodeling complex.
1999,
Pubmed
,
Xenbase
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Lusser,
Distinct activities of CHD1 and ACF in ATP-dependent chromatin assembly.
2005,
Pubmed
Längst,
Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.
1999,
Pubmed
Längst,
Nucleosome mobilization and positioning by ISWI-containing chromatin-remodeling factors.
2001,
Pubmed
Makarov,
The role of histone H1 and non-structured domains of core histones in maintaining the orientation of nucleosomes within the chromatin fiber.
1984,
Pubmed
Mizuguchi,
ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
2004,
Pubmed
Montel,
Atomic force microscopy imaging of SWI/SNF action: mapping the nucleosome remodeling and sliding.
2007,
Pubmed
,
Xenbase
Montel,
RSC remodeling of oligo-nucleosomes: an atomic force microscopy study.
2011,
Pubmed
Mutskov,
Persistent interactions of core histone tails with nucleosomal DNA following acetylation and transcription factor binding.
1998,
Pubmed
Peterson,
Promoter targeting and chromatin remodeling by the SWI/SNF complex.
2000,
Pubmed
Peterson,
Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription.
1992,
Pubmed
Scrittori,
pEg2 aurora-A kinase, histone H3 phosphorylation, and chromosome assembly in Xenopus egg extract.
2001,
Pubmed
,
Xenbase
Shukla,
Remosomes: RSC generated non-mobilized particles with approximately 180 bp DNA loosely associated with the histone octamer.
2010,
Pubmed
,
Xenbase
Shukla,
The docking domain of histone H2A is required for H1 binding and RSC-mediated nucleosome remodeling.
2011,
Pubmed
Smith,
Structural analysis of the yeast SWI/SNF chromatin remodeling complex.
2003,
Pubmed
Stefanovsky VYu,
Laser-induced crosslinking of histones to DNA in chromatin and core particles: implications in studying histone-DNA interactions.
1989,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Syed,
The incorporation of the novel histone variant H2AL2 confers unusual structural and functional properties of the nucleosome.
2009,
Pubmed
,
Xenbase
Syed,
Single-base resolution mapping of H1-nucleosome interactions and 3D organization of the nucleosome.
2010,
Pubmed
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Wu,
A 'one-pot' assay for the accessibility of DNA in a nucleosome core particle.
2004,
Pubmed
Yang,
The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing.
2006,
Pubmed
Zhang,
DNA translocation and loop formation mechanism of chromatin remodeling by SWI/SNF and RSC.
2006,
Pubmed
Zhou,
The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding.
2018,
Pubmed
de la Barre,
The N-terminus of histone H2B, but not that of histone H3 or its phosphorylation, is essential for chromosome condensation.
2001,
Pubmed
,
Xenbase
