Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The Xenopus embryo is a classical vertebrate model for molecular, cellular, and developmental biology. Despite many advantages of this organism, such as large egg size and external development, imaging of early embryonic stages is challenging because of nontransparent cytoplasm. Staining and imaging of thin tissue sections is one way to overcome this limitation. Here we describe a step-by-step protocol that combines cryosectioning of gelatin-embedded embryos with immunostaining and imaging. The purpose of this protocol is to examine various cellular and tissue markers after the manipulation of protein function. This protocol can be performed within a 2-d period and allows detection of many antigens by immunofluorescence.
Chalmers,
Oriented cell divisions asymmetrically segregate aPKC and generate cell fate diversity in the early Xenopus embryo.
2003, Pubmed,
Xenbase
Chalmers,
Oriented cell divisions asymmetrically segregate aPKC and generate cell fate diversity in the early Xenopus embryo.
2003,
Pubmed
,
Xenbase
Chu,
Prickle3 synergizes with Wtip to regulate basal body organization and cilia growth.
2016,
Pubmed
,
Xenbase
Dent,
A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus.
1989,
Pubmed
,
Xenbase
Dollar,
Regulation of Lethal giant larvae by Dishevelled.
2005,
Pubmed
,
Xenbase
Fagotto,
Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries.
1994,
Pubmed
,
Xenbase
Fischer,
Paraffin embedding tissue samples for sectioning.
2008,
Pubmed
Khudyakov,
Comprehensive spatiotemporal analysis of early chick neural crest network genes.
2009,
Pubmed
Kim,
Rab11 regulates planar polarity and migratory behavior of multiciliated cells in Xenopus embryonic epidermis.
2012,
Pubmed
,
Xenbase
Nandadasa,
N- and E-cadherins in Xenopus are specifically required in the neural and non-neural ectoderm, respectively, for F-actin assembly and morphogenetic movements.
2009,
Pubmed
,
Xenbase
Neil,
Fluorescence In Situ Hybridization of Cryosectioned Xenopus Oocytes.
2018,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Ossipova,
Role of Rab11 in planar cell polarity and apical constriction during vertebrate neural tube closure.
2014,
Pubmed
,
Xenbase
Ossipova,
PAR1 specifies ciliated cells in vertebrate ectoderm downstream of aPKC.
2007,
Pubmed
,
Xenbase