Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Genes Environ
2021 Nov 06;431:49. doi: 10.1186/s41021-021-00223-0.
Show Gene links
Show Anatomy links
Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis.
Qi X
,
Geng X
,
Zhang J
,
Qu B
,
Zhang X
,
Jia Q
,
Yin W
,
Bo C
,
Liu Y
,
Li H
,
Sai L
,
Han M
,
Peng C
.
???displayArticle.abstract???
BACKGROUND: Increasing evidence suggested N6-methyladenosine (m6A) modification is crucial for male germline development. However, m6A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m6A is a highly conserved modification across the species.
RESULTS: The results of m6A sequences showed that m6A-methylated lncRNAs enriched in intergenic region in testes of X. laevis. We further examined the differential expression of lncRNAs m6A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m6A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m6A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m6A-methylated mRNAs.
CONCLUSIONS: These findings suggested that differentially m6A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provides insights into the profile of lncRNAs m6A modifications in amphibian species.
???displayArticle.pubmedLink???
34742356
???displayArticle.link???Genes Environ ???displayArticle.grants???[+]
202012010629 health commission of shandong province, 202019162 ji'nan science and technology bureau, 81573198 national natural science foundation of china, 30901214 national natural science foundation of china, 2019QL001 the innovation project of shandong academy of medical sciences, academic promotion programme of shandong first medical university, 2018ZX09711001-011 ministry of science and technology of prc
Auer,
Statistical design and analysis of RNA sequencing data.
2010, Pubmed
Auer,
Statistical design and analysis of RNA sequencing data.
2010,
Pubmed
Ban,
LNCAROD is stabilized by m6A methylation and promotes cancer progression via forming a ternary complex with HSPA1A and YBX1 in head and neck squamous cell carcinoma.
2020,
Pubmed
Bassham,
SNAREs: cogs and coordinators in signaling and development.
2008,
Pubmed
Bedford,
Ubiquitin-like protein conjugation and the ubiquitin-proteasome system as drug targets.
2011,
Pubmed
Bhan,
Long noncoding RNAs: emerging stars in gene regulation, epigenetics and human disease.
2014,
Pubmed
Brahmaraju,
Spatio-temporal organization of Vam6P and SNAP on mouse spermatozoa and their involvement in sperm-zona pellucida interactions.
2004,
Pubmed
Cai,
RNA N6-methyladenosine modification, spermatogenesis, and human male infertility.
2021,
Pubmed
Campanoni,
Membrane trafficking and polar growth in root hairs and pollen tubes.
2007,
Pubmed
Coker,
m6A modification of non-coding RNA and the control of mammalian gene expression.
2019,
Pubmed
Daste,
Structure and function of longin SNAREs.
2015,
Pubmed
Deng,
Widespread occurrence of N6-methyladenosine in bacterial mRNA.
2015,
Pubmed
Dominissini,
Transcriptome-Wide Mapping of N⁶-Methyladenosine by m⁶A-Seq.
2015,
Pubmed
Engel,
The Role of m6A/m-RNA Methylation in Stress Response Regulation.
2018,
Pubmed
Esteller,
Non-coding RNAs in human disease.
2011,
Pubmed
Fagerstone,
Review of issues concerning the use of reproductive inhibitors, with particular emphasis on resolving human-wildlife conflicts in North America.
2010,
Pubmed
Freiman,
Regulating the regulators: lysine modifications make their mark.
2003,
Pubmed
Halvorson,
PACAP modulates GnRH signaling in gonadotropes.
2014,
Pubmed
Hanson,
Effects of atrazine on fish, amphibians, and reptiles: update of the analysis based on quantitative weight of evidence.
2019,
Pubmed
Hayes,
Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis).
2010,
Pubmed
,
Xenbase
He,
ALKBH5 Inhibits Pancreatic Cancer Motility by Decreasing Long Non-Coding RNA KCNK15-AS1 Methylation.
2018,
Pubmed
Huang,
m6A RNA methylation regulators could contribute to the occurrence of chronic obstructive pulmonary disease.
2020,
Pubmed
Joshi,
Long non-coding RNAs (lncRNAs) in spermatogenesis and male infertility.
2020,
Pubmed
Kasowitz,
Nuclear m6A reader YTHDC1 regulates alternative polyadenylation and splicing during mouse oocyte development.
2018,
Pubmed
Kerscher,
Modification of proteins by ubiquitin and ubiquitin-like proteins.
2006,
Pubmed
Kim,
HISAT: a fast spliced aligner with low memory requirements.
2015,
Pubmed
Kong,
Degradation of atrazine by UV/chlorine: Efficiency, influencing factors, and products.
2016,
Pubmed
Kornienko,
Gene regulation by the act of long non-coding RNA transcription.
2013,
Pubmed
Lan,
KIAA1429 contributes to liver cancer progression through N6-methyladenosine-dependent post-transcriptional modification of GATA3.
2019,
Pubmed
Langmead,
Fast gapped-read alignment with Bowtie 2.
2012,
Pubmed
Li,
m6A mRNA methylation controls T cell homeostasis by targeting the IL-7/STAT5/SOCS pathways.
2017,
Pubmed
Lin,
Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis.
2017,
Pubmed
Linder,
Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.
2015,
Pubmed
Liu,
RETRACTED: Long non-coding RNA TTN-AS1 promotes tumorigenesis of ovarian cancer through modulating the miR-139-5p/ROCK2 axis.
2020,
Pubmed
Liu,
LITHOPHONE: Improving lncRNA Methylation Site Prediction Using an Ensemble Predictor.
2020,
Pubmed
Liu,
m6A-induced lncRNA MALAT1 aggravates renal fibrogenesis in obstructive nephropathy through the miR-145/FAK pathway.
2020,
Pubmed
McDowell,
Ubiquitin-mediated proteolysis in Xenopus extract.
2016,
Pubmed
,
Xenbase
Meyer,
Rethinking m6A Readers, Writers, and Erasers.
2017,
Pubmed
Meyer,
5' UTR m(6)A Promotes Cap-Independent Translation.
2015,
Pubmed
Meyer,
Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons.
2012,
Pubmed
Moran,
Emerging functional and mechanistic paradigms of mammalian long non-coding RNAs.
2012,
Pubmed
Moreno,
Vesicular traffic and golgi apparatus dynamics during mammalian spermatogenesis: implications for acrosome architecture.
2000,
Pubmed
Nagarajan,
Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA.
2019,
Pubmed
Niu,
N6-methyl-adenosine (m6A) in RNA: an old modification with a novel epigenetic function.
2013,
Pubmed
Ojeda,
Puberty in 2013: Unravelling the mystery of puberty.
2014,
Pubmed
Orton,
Effects of nitrate and atrazine on larval development and sexual differentiation in the northern leopard frog Rana pipiens.
2006,
Pubmed
Ramalho-Santos,
Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis.
2001,
Pubmed
Rohr,
A qualitative meta-analysis reveals consistent effects of atrazine on freshwater fish and amphibians.
2010,
Pubmed
Sai,
Gene expression profiles in testis of developing male Xenopus laevis damaged by chronic exposure of atrazine.
2016,
Pubmed
,
Xenbase
Sai,
Distinct m6A methylome profiles in poly(A) RNA from Xenopus laevis testis and that treated with atrazine.
2020,
Pubmed
,
Xenbase
Sai,
Analysis of long non-coding RNA involved in atrazine-induced testicular degeneration of Xenopus laevis.
2019,
Pubmed
,
Xenbase
Solomon,
Ecological risk assessment of atrazine in North American surface waters.
2013,
Pubmed
Storrs,
Survivorship patterns of larval amphibians exposed to low concentrations of atrazine.
2004,
Pubmed
Sutovsky,
Ubiquitin-dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: killing three birds with one stone.
2003,
Pubmed
Suzuki,
Differential distribution of orexin-A-like and orexin receptor 1 (OX1R)-like immunoreactivities in the Xenopus pituitary.
2007,
Pubmed
,
Xenbase
Swatek,
Ubiquitin modifications.
2016,
Pubmed
Tavera-Mendoza,
Response of the amphibian tadpole (Xenopus laevis) to atrazine during sexual differentiation of the testis.
2002,
Pubmed
,
Xenbase
Tu,
N6-Methylandenosine-Related lncRNAs Are Potential Biomarkers for Predicting the Overall Survival of Lower-Grade Glioma Patients.
2020,
Pubmed
Wang,
Bioinformatics analysis integrating metabolomics of m6A RNA microarray in intervertebral disc degeneration.
2020,
Pubmed
Wang,
Molecular mechanisms of long noncoding RNAs.
2011,
Pubmed
Wu,
m6A-induced lncRNA RP11 triggers the dissemination of colorectal cancer cells via upregulation of Zeb1.
2019,
Pubmed
Xiao,
Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing.
2016,
Pubmed
Xiao,
The RNA N6-methyladenosine modification landscape of human fetal tissues.
2019,
Pubmed
Yang,
N6-Methyladenosine modification of lincRNA 1281 is critically required for mESC differentiation potential.
2018,
Pubmed
Yu,
Dynamic m6A modification regulates local translation of mRNA in axons.
2018,
Pubmed
Zhang,
m6A modulates haematopoietic stem and progenitor cell specification.
2017,
Pubmed
Zhao,
Increased m6A RNA modification is related to the inhibition of the Nrf2-mediated antioxidant response in di-(2-ethylhexyl) phthalate-induced prepubertal testicular injury.
2020,
Pubmed
Zhu,
Function and evolution of RNA N6-methyladenosine modification.
2020,
Pubmed
de la Casa-Resino,
Endocrine disruption caused by oral administration of atrazine in European quail (Coturnix coturnix coturnix).
2012,
Pubmed
