Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochem Biophys Res Commun
2022 Jun 18;609:156-162. doi: 10.1016/j.bbrc.2022.04.015.
Show Gene links
Show Anatomy links
Odorant inhibition in mosquito olfaction mediated by inverse agonists.
Xu P
,
Choo YM
,
Leal WS
.
???displayArticle.abstract???
The insect repellent methyl salicylate elicits excitatory responses upon interaction with CquiOR32, an odorant receptor (OR) from the southern house mosquito, Culex quinquefasciatus. By contrast, eucalyptol binds to CquiOR32 to generate electrophysiological and behavioral inhibitory responses. In an attempt to identify CquiOR32 variants displaying more robust inhibitory responses for more accurate current-voltage analysis, we sequenced 31 CquiOR32 clones. In the Xenopus oocyte recording system, CquiOR32V2/CquiOrco-expressing oocytes yielded eucalyptol-elicited outward (inhibitory) currents relatively larger than methyl salicylate-generated inward (excitatory) currents. Rescuing experiments showed that two of the amino acid substitutions in CquiOR32V2 located in a predicted transmembrane helix of the receptor are determinants of the outward/inward ratios. These findings, along with co-stimulus assays, suggest that odorant and inhibitor may bind to the same binding pocket. Current-voltage relationships obtained with standard perfusion buffer and those devoid of Na+ or Cl- indicated that both excitatory and inhibitory currents are mediated, at least in part, by cation. We then concluded that eucalyptol is an inverse agonist, which shifts the open ⇔ closed equilibrium of the receptor toward the closed conformation, thus reducing the spontaneous activity. By contrast, the binding of methyl salicylate shifts the equilibrium towards the open conformation and, consequently, leads to an increase in cation influx.
Baker,
Behavioral antagonism in the moth Helicoverpa zea in response to pheromone blends of three sympatric heliothine moth species is explained by one type of antennal neuron.
1998, Pubmed
Baker,
Behavioral antagonism in the moth Helicoverpa zea in response to pheromone blends of three sympatric heliothine moth species is explained by one type of antennal neuron.
1998,
Pubmed
Cao,
Odor-evoked inhibition of olfactory sensory neurons drives olfactory perception in Drosophila.
2017,
Pubmed
Chang,
A Pheromone Antagonist Regulates Optimal Mating Time in the Moth Helicoverpa armigera.
2017,
Pubmed
Chen,
Identification of new agonists and antagonists of the insect odorant receptor co-receptor subunit.
2012,
Pubmed
,
Xenbase
Choo,
Reverse chemical ecology approach for the identification of an oviposition attractant for Culex quinquefasciatus.
2018,
Pubmed
,
Xenbase
Del Mármol,
The structural basis of odorant recognition in insect olfactory receptors.
2021,
Pubmed
Ghaninia,
Functional classification and central nervous projections of olfactory receptor neurons housed in antennal trichoid sensilla of female yellow fever mosquitoes, Aedes aegypti.
2007,
Pubmed
Hartzell,
Calcium-activated chloride channels.
2005,
Pubmed
Hildebrand,
Olfactory control of behavior in moths: central processing of odor information and the functional significance of olfactory glomeruli.
1996,
Pubmed
Jones,
Functional agonism of insect odorant receptor ion channels.
2011,
Pubmed
Leal,
Reverse and conventional chemical ecology approaches for the development of oviposition attractants for Culex mosquitoes.
2008,
Pubmed
Leary,
Single mutation to a sex pheromone receptor provides adaptive specificity between closely related moth species.
2012,
Pubmed
,
Xenbase
Lee,
High degree of single nucleotide polymorphisms in California Culex pipiens (Diptera: Culicidae) sensu lato.
2012,
Pubmed
Lotan,
Adenosine-induced slow ionic currents in the Xenopus oocyte.
1982,
Pubmed
,
Xenbase
Menini,
Olfactory Information Processing in Moths
2010,
Pubmed
Menini,
Odor Coding in Insects
2010,
Pubmed
Mucignat-Caretta,
Pheromone Reception in Insects: The Example of Silk Moths
2014,
Pubmed
Nichols,
Transmembrane segment 3 of Drosophila melanogaster odorant receptor subunit 85b contributes to ligand-receptor interactions.
2010,
Pubmed
,
Xenbase
Nikonov,
Peripheral coding of sex pheromone and a behavioral antagonist in the Japanese beetle, Popillia japonica.
2002,
Pubmed
Paluch,
Mosquito repellents: a review of chemical structure diversity and olfaction.
2010,
Pubmed
Pask,
Blockade of insect odorant receptor currents by amiloride derivatives.
2013,
Pubmed
Pask,
Heteromeric Anopheline odorant receptors exhibit distinct channel properties.
2011,
Pubmed
Reynolds,
Transmembrane topology and signal peptide prediction using dynamic bayesian networks.
2008,
Pubmed
Sato,
Insect olfactory receptors are heteromeric ligand-gated ion channels.
2008,
Pubmed
,
Xenbase
Schroeder,
Expression cloning of TMEM16A as a calcium-activated chloride channel subunit.
2008,
Pubmed
,
Xenbase
Smart,
Drosophila odorant receptors are novel seven transmembrane domain proteins that can signal independently of heterotrimeric G proteins.
2008,
Pubmed
Stensmyr,
A conserved dedicated olfactory circuit for detecting harmful microbes in Drosophila.
2012,
Pubmed
Su,
Non-synaptic inhibition between grouped neurons in an olfactory circuit.
2012,
Pubmed
Syed,
Acute olfactory response of Culex mosquitoes to a human- and bird-derived attractant.
2009,
Pubmed
Tauxe,
Targeting a dual detector of skin and CO2 to modify mosquito host seeking.
2013,
Pubmed
Taylor,
Structure-activity relationship of a broad-spectrum insect odorant receptor agonist.
2012,
Pubmed
Wicher,
Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels.
2008,
Pubmed
Wu,
Valence opponency in peripheral olfactory processing.
2022,
Pubmed
Xu,
Odorant Inhibition in Mosquito Olfaction.
2019,
Pubmed
Xu,
DEET and other repellents are inhibitors of mosquito odorant receptors for oviposition attractants.
2019,
Pubmed
,
Xenbase
Xu,
Mosquito odorant receptor sensitive to natural spatial repellents and inhibitory compounds.
2022,
Pubmed
Xu,
CO2 per se activates carbon dioxide receptors.
2020,
Pubmed
,
Xenbase
Ye,
Olfactory Responses of Southern House Mosquito, Culex quinquefasciatus, to Human Odorants.
2016,
Pubmed
Zhu,
Identification of odors from overripe mango that attract vinegar flies, Drosophila melanogaster.
2003,
Pubmed