Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Methods Mol Biol
2022 Jan 01;2502:51-66. doi: 10.1007/978-1-0716-2337-4_3.
Show Gene links
Show Anatomy links
Nuclear Pore Complex Assembly Using Xenopus Egg Extract.
Holzer G
,
Antonin W
.
???displayArticle.abstract???
Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study nuclear pore complex assembly and how to analyze the process using Western blot or immunofluorescence based assays. The function of proteins can be conveniently assayed by high-efficient antibody mediated depletion.
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986, Pubmed,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Brown,
Chromatin decondensation and DNA synthesis in human sperm activated in vitro by using Xenopus laevis egg extracts.
1987,
Pubmed
,
Xenbase
Dabauvalle,
Assembly of nuclear pore complexes in Xenopus egg extract.
1991,
Pubmed
,
Xenbase
Gordon,
In vitro activation of human sperm induced by amphibian egg extract.
1985,
Pubmed
,
Xenbase
Guadagno,
Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase
Magalska,
RuvB-like ATPases function in chromatin decondensation at the end of mitosis.
2014,
Pubmed
,
Xenbase
Rasala,
Capture of AT-rich chromatin by ELYS recruits POM121 and NDC1 to initiate nuclear pore assembly.
2008,
Pubmed
,
Xenbase
Theerthagiri,
The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex.
2010,
Pubmed
,
Xenbase
Vollmer,
Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly.
2012,
Pubmed
,
Xenbase
Vollmer,
Nup153 Recruits the Nup107-160 Complex to the Inner Nuclear Membrane for Interphasic Nuclear Pore Complex Assembly.
2015,
Pubmed
,
Xenbase
Yokoyama,
The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation.
2014,
Pubmed
,
Xenbase
Zanni,
Biallelic Variants in the Nuclear Pore Complex Protein NUP93 Are Associated with Non-progressive Congenital Ataxia.
2019,
Pubmed
