Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Marginal zone explants from Xenopus embryos can be used to expose cell behaviors and tissue movements that normally operate in dorsal tissues. Dorsal explants comprise the diverse set of progenitor cells found in dorsal tissues including mesendoderm, headmesoderm, prechordal mesoderm, endoderm with bottle cells, axial mesoderm of the prospective notochord, paraxial mesoderm of the somites, lateral plate mesoderm, neural ectoderm, and ectoderm. Unlike an organoid, the dorsal marginal zone (DMZ) explant is "organotypic" in that microsurgery does not disrupt native tissue organization beyond manipulations needed to dissect the tissue from the embryo. An organotypic early gastrulaDMZ explant preserves boundaries and close tissue associations in the native marginal zone. Depending on the stage, patterning and cell identities can be maintained in explants and tissue isolates. Local cell movements and behaviors may also be preserved; however, the large-scale biomechanical impact of their collective movements may be altered from those in the native marginal zone. For instance, involution is typically inhibited in the DMZ explant, precluding the two-layer association of mesoderm and prospective neural ectoderm normally achieved during gastrulation. DMZ explants may be mounted and imaged in a variety of ways, exposing interesting cell behaviors or collective movements such as mediolateral cell intercalation in the axial and paraxial mesoderm, apical constriction of bottle cells, and directional migration of mesendoderm. The flattened DMZ explant can also be used to study emergence of new tissue-defining boundaries such as the notochord-somite boundary, the ectoderm-mesoderm boundary, and the mesendoderm-mesoderm boundary.
Chu,
Chambers for Culturing and Immobilizing Xenopus Embryos and Organotypic Explants for Live Imaging.
2022, Pubmed,
Xenbase
Chu,
Chambers for Culturing and Immobilizing Xenopus Embryos and Organotypic Explants for Live Imaging.
2022,
Pubmed
,
Xenbase
Davidson,
Mesendoderm extension and mantle closure in Xenopus laevis gastrulation: combined roles for integrin alpha(5)beta(1), fibronectin, and tissue geometry.
2002,
Pubmed
,
Xenbase
Davidson,
Patterning and tissue movements in a novel explant preparation of the marginal zone of Xenopus laevis.
2004,
Pubmed
,
Xenbase
Davidson,
Assembly and remodeling of the fibrillar fibronectin extracellular matrix during gastrulation and neurulation in Xenopus laevis.
2004,
Pubmed
,
Xenbase
Davidson,
Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension.
2006,
Pubmed
,
Xenbase
DeSimone,
The Xenopus embryo as a model system for studies of cell migration.
2005,
Pubmed
,
Xenbase
Domingo,
Induction of notochord cell intercalation behavior and differentiation by progressive signals in the gastrula of Xenopus laevis.
1995,
Pubmed
,
Xenbase
Domingo,
Cells remain competent to respond to mesoderm-inducing signals present during gastrulation in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Gillespie,
The distribution of small ions during the early development of Xenopus laevis and Ambystoma mexicanum embryos.
1983,
Pubmed
,
Xenbase
Goto,
Planar cell polarity genes regulate polarized extracellular matrix deposition during frog gastrulation.
2005,
Pubmed
,
Xenbase
Hellsten,
The genome of the Western clawed frog Xenopus tropicalis.
2010,
Pubmed
,
Xenbase
Huebner,
Mechanical heterogeneity along single cell-cell junctions is driven by lateral clustering of cadherins during vertebrate axis elongation.
2021,
Pubmed
,
Xenbase
Kakebeen,
A temporally resolved transcriptome for developing "Keller" explants of the Xenopus laevis dorsal marginal zone.
2021,
Pubmed
,
Xenbase
Keller,
Mechanisms of convergence and extension by cell intercalation.
2000,
Pubmed
Keller,
The function and mechanism of convergent extension during gastrulation of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Kim,
Punctuated actin contractions during convergent extension and their permissive regulation by the non-canonical Wnt-signaling pathway.
2011,
Pubmed
,
Xenbase
Kim,
Assembly of chambers for stable long-term imaging of live Xenopus tissue.
2013,
Pubmed
,
Xenbase
Marsden,
Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus.
2003,
Pubmed
,
Xenbase
Pfister,
Molecular model for force production and transmission during vertebrate gastrulation.
2016,
Pubmed
,
Xenbase
Poznanski,
The role of planar and early vertical signaling in patterning the expression of Hoxb-1 in Xenopus.
1997,
Pubmed
,
Xenbase
Poznanski,
Epithelial cell wedging and neural trough formation are induced planarly in Xenopus, without persistent vertical interactions with mesoderm.
1997,
Pubmed
,
Xenbase
Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase
Shih,
Cell motility driving mediolateral intercalation in explants of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Shih,
Patterns of cell motility in the organizer and dorsal mesoderm of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Shindo,
PCP-dependent transcellular regulation of actomyosin oscillation facilitates convergent extension of vertebrate tissue.
2019,
Pubmed
,
Xenbase
Shindo,
PCP and septins compartmentalize cortical actomyosin to direct collective cell movement.
2014,
Pubmed
,
Xenbase
Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase