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Development January 1, 2022; 149 (13):
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Distinct spatiotemporal contribution of morphogenetic events and mechanical tissue coupling during Xenopus neural tube closure.

Christodoulou N , Skourides PA .

Neural tube closure (NTC) is a fundamental process during vertebrate development and is indispensable for the formation of the central nervous system. Here, using Xenopus laevis embryos, live imaging, single-cell tracking, optogenetics and loss-of-function experiments, we examine the roles of convergent extension and apical constriction, and define the role of the surface ectoderm during NTC. We show that NTC is a two-stage process with distinct spatiotemporal contributions of convergent extension and apical constriction at each stage. Convergent extension takes place during the first stage and is spatially restricted at the posterior tissue, whereas apical constriction occurs during the second stage throughout the neural plate. We also show that the surface ectoderm is mechanically coupled with the neural plate and its movement during NTC is driven by neural plate morphogenesis. Finally, we show that an increase in surface ectoderm resistive forces is detrimental for neural plate morphogenesis.

PubMed ID: 35662330
Article link: Development
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: h2bc21 itgb1 prickle2 prok2 shroom3 sox3 vangl2
GO keywords: neural tube closure [+]
Antibodies: Itgb1 Ab1 Sox3 Ab3
Morpholinos: itgb1 MO1 shroom3 MO1 vangl2 MO2

Article Images: [+] show captions
References [+] :
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